LOCUS       sequence-449793-        8758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor TCF12 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8758)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-TCF12-Brd 50
FEATURES             Location/Qualifiers
     source          1..8758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    371..496
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             543..723
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(724..950)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(978..998)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(978..996)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1017..1033)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1017..1033)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1030..1052)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1041..1057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1065..1071,1072..1089,1090..1095))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1110..1131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1248..1265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1419..2007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1499..1518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2178..2969,2970..3038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2801..2820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3039..3143)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3169..3624)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3306..3327)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3518..3537
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3751..3773
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3765..3782
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3766..3782
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3792..3811
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3792..3810
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3831..3966
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4006..4127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4017..4036)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4071..4090
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4199..4432
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4551..4954
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(7350..7756)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    7790..7907
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8174..8218)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8403..8636
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt
       61 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
      121 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
      181 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
      241 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
      301 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
      361 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
      421 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
      481 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
      541 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
      601 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
      661 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
      721 cccgtttatg tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt
      781 ggttcgtcca atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca
      841 ccttacttcc accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta
      901 actcatcgtt accatgttgc aagactacaa gagtattgca taagactaca ttaagcttgc
      961 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     1021 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1081 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1141 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1201 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2161 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2221 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2281 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2341 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2401 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2461 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2521 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2581 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2641 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2701 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2761 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2821 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2881 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2941 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3001 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3061 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     3181 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3241 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3301 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3361 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3421 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3481 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3541 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3601 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     3661 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3721 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3781 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacgtcccta ggcctccaaa
     3841 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     3901 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     3961 cgggatgggc ggagttaggg gcgggatagc tagagccaga catgataaga tacattgatg
     4021 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4081 atgctattgc tttatttgta accattataa gctgcaataa acaagttcct ctcactctct
     4141 gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga actactactg
     4201 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     4261 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     4321 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     4381 gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     4441 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tctagaagcg
     4501 ctattacgca tgtacatcta gaaggtacag cttgatatcg aattgcaaag gacgtcagct
     4561 tccccgacaa caccacggaa ttatcagtgc ccagcaacct agcccctgtc cagcagcggg
     4621 caaggcaggc ggcgatgagt tctgccgtgg cgatcgggag ggggaaagcg aaagtcccag
     4681 aaaggagttg acaggtggtg gcaatgcccc agccagtggg ggttgcgtca gcaaacacag
     4741 agcacaccac gccacgttga cggacaacgg gccacaactc ctcataaaga gacagcaacc
     4801 aggatttata caaggaggag aaaacgaaag ccgtacggga agcaatagca tgatacagag
     4861 gcattaaagc agcatatcca cacagcgtaa aaggagcaac atagttaaga atatcagtca
     4921 atctttcaca aattttgtaa tccagaggtt gattaacagg aacagagcgt aaataacggg
     4981 aaagtttctt aacatgtttg tcttgtggca atacacctga actagtaatt acatatccct
     5041 aaaaatgtaa atgattgccc caccattttg ttttattaac atttaaatgt atacccaaat
     5101 caagaaaaac agaacaaata tgggaataaa tggcggtaag atgctcgaat tcctgcagcc
     5161 cgggggatcc gaccggttac atatgaccca tagggttggt agtttcacta agcccaggat
     5221 gggttcctgg cagtgtggtt ggcggctctg ccgatacggc agaaactttt tcttcttccc
     5281 ttctcttaag gcaggctgct ttggggttaa ggttcctctc tctgacttgc tgttctagac
     5341 taaggatgac tgccacggct tgatgaagaa taaggagttt tgtttggggt ttttcactct
     5401 tcaagtgaag ctgacacatt cggccaagct ctttgaatgc ttcattaata tcccgcacgc
     5461 gtaagcgttc tctggcattg ttagccatcc gcctctcctt ctccctttct atcttctgtt
     5521 cagggttcaa atcctcatct tcattagtac tgcttgttct gcctctagat gaaaccttga
     5581 tatctttttg ggaggattca tcatctgact tcatgtcatc tgatgaagga ggttcatgaa
     5641 ggttttcatc cttttctttg ttttcagtct tgatttctgt tgtaacaaca gttccagact
     5701 gactttgcaa gccacctcta taattttctt gtgttttatg gttcaggtct gtgcttgaag
     5761 tagtgactgt actagacagg actgaatgat tgccattgag actgacagag tcttcccgat
     5821 gagttccaac cattgaagct gatcgactgc ttgcaacaag gcttgatcct ccatagtttg
     5881 aattgaggct tccaattggt gcattatggg atggtcccaa taaactatgt atatcactgt
     5941 gaccagcagg caaactggtg gaaggtccca cagcatggtt ccgcagcaca tggattgcat
     6001 catccagtct gtctaaacga tcctccattc gagactgctc acagacatcc tttaagaagg
     6061 acattgcatc ttgcaaatgc tcgtgaagtt gctgctcaac tcgatttttc agggagtgga
     6121 gtgagttttc atagcttggg gatgaaggtg cttgccctcc aggtcttggc cactgactgg
     6181 tacctgtgag aggtgaaggt gatccaactg gtgttgatgg atttgacgga aaactactgc
     6241 tggtatggtc aggagaataa atagatgcca aagcctttcc aagtgcatca cctgtctgtg
     6301 agcttccagc agcattccct ctggttccta gaatgctgtc tgatccattg atgggaggag
     6361 tgtgtgaggc agcaacgtaa ggtgaactgc tggtactgcc gcgatgaaag ctggacattg
     6421 gtggaagact cgtgtttatg tctgttggtg aaactgagtg tggaggataa ctcaagcggt
     6481 catgtgaatg aaggttgcca taactactgg attgagacat gtgggaagtg gaggtcccca
     6541 gaattccacc aaaaccaggc tggctcatcc catttgatga actccaaagg tcagaagaat
     6601 tgtgggtccc atcttgcata aagaaagtgc tagcgaacat actggttggt ggcttaggag
     6661 atggataact aggagattca cggttgaaat catctgaatt tggggatggt gcatatacag
     6721 aagaaggcaa accaggaggc acctttctga ctttttttgc ttgcaaggga tcaagcgctg
     6781 cagagtcatg gagtggtctc ctcctggaac ttgtagcaga gaatgaatag tatgctgtcc
     6841 caggttttcc tgaagaagat agctgtgctg ggctcccaag ccccagatct tgtctcagga
     6901 gactagattg acagcctggt aatccagtat ctctgctgta cagggaaaat gagcctctct
     6961 ctgatgtttt tcccatcaga tttgagttca tgaaaggtgt tggggacaag ccttcatggg
     7021 ctcctaatcg actgtcattc aagtgatcac tgtaatgagg gctgtctgta aaacctctag
     7081 atgaatcata ggaaggactt ggttgaccac ttgttcccca agatgttgta cctcctcttt
     7141 catcaatacc tgatccactg aactgactgc ttcccagtgt agttggtcta gttttcccac
     7201 tattaacagg tggggaaaac atcgcactga agtccagtag gtcgctcagc tccttgtcgg
     7261 tccctatagc ggccatgcgt tgttgctggg gattcatttc gaaccgcggg ctagcggatc
     7321 cgtcgacggt atcgataagc ttgattcgac cggccgactc agtctgtcgg aggactggcg
     7381 cgccgagtga ggggttgtga gctcttttat agagctcggg aagcagaagc gcgcgaacag
     7441 aagcgagaag caggctgatt ggttaattca aataaggcgc agggtcattt caggtccttg
     7501 ggggagcctg gaaacatctg atgggtctta agaaactgct gagggttggg ccatatctgg
     7561 ggaccatctg ttcttggcct cgggccgggg ccgaaactgc ggtgaccatc tgttcttggc
     7621 cccgggccgg ggccgaaact gctcaccgca gatatcctgt ttggcccaac gttagctatt
     7681 ttcatgtacc cgcccttgat ctgaacttct ctattcttgg tttggtattt ttccatgcct
     7741 tgcaaaatgg cgttactgca gccagctcga ggctagtctc gtgatcgata aaattttgaa
     7801 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     7861 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaagtt aaccgatacc
     7921 gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
     7981 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
     8041 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
     8101 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
     8161 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
     8221 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     8281 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
     8341 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
     8401 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
     8461 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
     8521 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
     8581 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
     8641 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
     8701 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcat
//
