LOCUS       sequence-449799-        8341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1) Ectopic expression of MAAP6 protein in mammalian cells. 2) 
            Packaging MAAP6 sequence into lentivirus for stable expression in 
            mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Schieferecke AJ, Lee H, Chen A, Kilaru V, Krish Williams J, Schaffer
            DV
  TITLE     Evolving membrane-associated accessory protein variants for improved
            adeno-associated virus production.
  JOURNAL   Mol Ther. 2024 Feb 7;32(2):340-351. doi: 
            10.1016/j.ymthe.2023.12.015. Epub 2023 Dec 18.
  PUBMED    38115579
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymthe.2023.12.015"; journalName: "Mol Ther"; date: 
            "2024-02-7- 7"; volume: "32"; issue: "2"; pages: "340-351"
COMMENT     Sequence Label: pCW57-CMV-MAAP6
FEATURES             Location/Qualifiers
     source          1..8341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    158..391
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    884..1009
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1056..1236
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1237..1463)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1491..1511)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1491..1509)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1530..1546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1530..1546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1543..1565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1554..1570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1578..1584,1585..1602,1603..1608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1623..1644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1761..1778)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1932..2520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2012..2031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2691..3482,3483..3551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3314..3333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3552..3623)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3623..3758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3798..3919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3809..3828)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3863..3882
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3991..4224
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             4429..4440
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4838..4858
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4898..4906,4907..4942,4943..4945,4946..4981,
                     4982..4984,4985..5020,5021..5026,5027..5644))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(5645..5698)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5699..6295)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5779..5799)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6276..6295
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6305..6815)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6368..6386)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      7205..7214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7239..7258)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7240..7258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7303..7506)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7332..7352)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7507..7886
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7940..8057
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8270..8314)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
       61 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
      121 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
      181 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
      241 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
      301 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
      361 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
      421 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
      481 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
      541 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
      601 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
      661 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
      721 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
      781 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
      841 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
      901 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
      961 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     1021 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     1081 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     1141 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     1201 ctcccaggct cagatctggt ctaaccagag agacccgttt atgtatcgag ctaggcactt
     1261 aaatacaata tctctgcaat gcggcaattc agtggttcgt ccaatccatg tcagacccgt
     1321 ctgttgcctt cctaataagg cacgatcgta ccaccttact tccaccaatc ggcatgcacg
     1381 gtgctttttc tctccttgta aggcatgttg ctaactcatc gttaccatgt tgcaagacta
     1441 caagagtatt gcataagact acattaagct tgcagctcca gcttttgttc cctttagtga
     1501 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1561 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1621 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1681 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1741 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1801 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1861 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2101 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2341 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2521 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2581 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2641 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2701 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2761 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2821 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2881 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2941 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3001 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3061 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3121 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3181 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3241 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3301 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3361 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3421 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3481 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3541 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3601 atgagcggat acatatttga aaggcctcca aaaaagcctc ctcactactt ctggaatagc
     3661 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     3721 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata
     3781 gctagagcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3841 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3901 aagctgcaat aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat
     3961 actgaataat aagatgacat gaactactac tgctagagat tttccacact gactaaaagg
     4021 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     4081 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     4141 aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc
     4201 gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta
     4261 cagctgcctt gtaagtcatt ggtcttaaag gtacctctag tccggacgcc gaggcgaaac
     4321 aggcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
     4381 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
     4441 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
     4501 tggaaaggac gtcagcttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
     4561 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
     4621 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
     4681 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     4741 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     4801 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     4861 gttaagaata cgatatcttg gatcgatcct tacttagtta cccggggagc atgtcaaggt
     4921 caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc aaagtcgtca agggcatcgg
     4981 ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggt cggcccgccg ctttcgcact
     5041 ttagctgttt ctccaggcca catatgatta gttccaggcc gaaaaggaag gcaggttcgg
     5101 ctccctgccg gtcgaacagc tcaattgctt gtctcagaag tgggggcata gaatcggtgg
     5161 taggtgtctc tctttcctct tttgctactt gatgctcctg ttcctccaat acgcagccca
     5221 gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt ctccagggag aagccttgct
     5281 gacacaggaa cgcgagctga ttttccaggg tttcgtactg tttctctgtt gggcgggtgc
     5341 cgagatgcac tttagccccg tcgcgatgtg agaggagagc acagcggtat gacttggcgt
     5401 tgttccgcag aaagtcttgc catgactcgc cttccagggg gcagaagtgg gtatgatgcc
     5461 tgtccagcat ctcgattggc agggcatcga gcagggcccg cttgttcttc acgtgccagt
     5521 acagggtagg ctgctcaact cccagctttt gagcgagttt ccttgtcgtc aggccttcga
     5581 taccgacacc attgagtaat tccagagctc cgtttatgac tttgctcttg tccagtctag
     5641 acattggacc agggttttct tcaacatcac cacaagtgag gagagaacct ctaccttcgg
     5701 caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg
     5761 tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga
     5821 aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca
     5881 gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct
     5941 tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc
     6001 tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg
     6061 gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga
     6121 gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg
     6181 gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg
     6241 ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtga
     6301 attgctgggg agagaggtcg gtgattcggt caacgaggga gccgactgcc gacgtgcgct
     6361 ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa cagggcccac actaccgccc
     6421 cacaccccgc ctcccgcacc gccccttccc ggccgctgct ctcggcgcgc cctgctgagc
     6481 agccgctatt ggccacagcc catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc
     6541 cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga
     6601 accgcaagga accttcccga cttaggggcg gagcaggaag cgtcgccggg gggcccacaa
     6661 gggtagcggc gaagatccgg gtgacgctgc gaacggacgt gaagaatgtg cgagacccag
     6721 ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc
     6781 cagggctgcc ttggaaaagg cgcaacccca accccggatc ctgtacaacg cgtctgcagc
     6841 ctaggttact accggacgtt tctttccagg agccgtctta gcaccttcct caaccagacc
     6901 aaaaggttcg agaaccctct tcttggcctg gaagactgct cgcccgaggt tgcccccaaa
     6961 agacgtatct tcttgcagac gctcctgaaa ctcggcgtcg gcgtggttat accgcaggta
     7021 cggattgtca cccgctttga gctgctggtc gtaggccttg tcgtgctcga gggccgctgc
     7081 atccgccgcg ttgacgggct cccccttgtc gagtccgttg aagggtccga ggtacttgta
     7141 gccaggaagc accagacccc ggccgtcgtc ctgcttttgc tggttggctt tgggtttcgg
     7201 ggctccatgg tggcaccggt ggtaccaagc ttgggtctcc ctatagtgag tcgtattaat
     7261 ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac
     7321 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     7381 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     7441 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     7501 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     7561 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     7621 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     7681 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     7741 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     7801 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     7861 ttaataacta gtcaataatc aatgtcaacg catcgattcg cggccaaagt ggatctctgc
     7921 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
     7981 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
     8041 ccccttttct tttaaaattg tggatgaata ctgccatttg tctcgaggtc gagaattgtc
     8101 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
     8161 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
     8221 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
     8281 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
     8341 t
//
