LOCUS       sequence-449829-       10495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express mouse Regnase-3 D252N mutant tagged 
            with FLAG-HA at N-term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Bataclan M, Leoni C, Moro SG, Pecoraro M, Wong EH, Heissmeyer V, 
            Monticelli S
  TITLE     Crosstalk between Regnase-1 and -3 shapes mast cell survival and 
            cytokine expression.
  JOURNAL   Life Sci Alliance. 2024 Jun 3;7(8):e202402784. doi: 
            10.26508/lsa.202402784. Print 2024 Aug.
  PUBMED    38830770
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2024-06-3"; volume: "7(8):e202402784. doi"; 
            pages: " 10.26508/lsa.202402784. Print 2024 Aug"
COMMENT     Sequence Label: pScalps-Puro-FLAG-HA-Regnase-3 D252N
FEATURES             Location/Qualifiers
     source          1..10495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..84)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        69..398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      249..384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     311..330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        410..789
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        791..989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     944..964
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1007..1187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1234..1359
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1856..2089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2273..2317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2615..2732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2869..3276
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3298..3307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3307..3330
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3343..3369
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3343..3369
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6601..7200
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6601..6620)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7097..7117
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7210..7798
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7263..7283)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7681..7692)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8316..8496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8567..9155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8647..8666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9326..10117,10118..10186))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9949..9968
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10187..10291)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10366..10385)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
       61 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      301 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
      361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgcgttg acattgatta
      421 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      481 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      541 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      601 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      661 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      721 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      781 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      841 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      901 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      961 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     1021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1201 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1261 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1321 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1381 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1441 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1501 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1561 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1621 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1681 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     1741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaattaacaa
     2221 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2281 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2341 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2401 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2461 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2521 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2581 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2641 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2701 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2761 cagtttggtt aattaactgc agccccgata aaataaaaga ttttatttag tctccagaaa
     2821 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt
     2881 tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca
     2941 tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc
     3001 cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg
     3061 gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct
     3121 cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc
     3181 gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc
     3241 ggcgcgccag tcctccgaca gactgagtcg cccgggggtc tagaagcgct aacgcgtgcc
     3301 accatggact acaaggacga cgatgacaag ctcgatggag gataccccta cgacgtgccc
     3361 gactacgccg gctcctccgg ctgtacaatg gctgcggaga agacattgca ggaatacggg
     3421 gtgctttgca ttcaagaata caggaaaagc agcaaagtgg agtcaagtgc acggaacagc
     3481 ttcatgggcc tgaaggatca cctggggcat gatcttggcc atctttatat ggagagcact
     3541 gacccacaga tgagtgcagc tgtgccttgg cctatggtag agaaaccaac aatggataca
     3601 gtcaattcgg ggaaggaagg aaaaggggtg tcagaagaga atgtgagctc cggcgactct
     3661 gagggaagca cgagttctga tcatgagtca gaacaactga gtagcctttc ggtagagcca
     3721 tgctcattaa ccaagactca cagacagcta tgtcgctctc cctgcttaga gcctcgccta
     3781 ctgaaacaca gtgacatttt gcaagacttt aaaccagaag agtcccagac tccatctaag
     3841 gaagtgaaga aaccccctga tgtggttcga gaataccaaa caaaactgga gtttgcactt
     3901 aagttaggtt attctgaaga acaggtccag cttgtgctaa acaaacttgg tactgatgct
     3961 ttaatcaatg atatattggg agaacttgtc aaacttggaa ataaaagtga agccgatcaa
     4021 acagttagta ctattaacag cgtaatgcga gaaacctcat ccctggagtc tcagaggtct
     4081 gaatctccaa tgcaagaggt tgtagtagat gacggtgaga atctgagacc agtagttatt
     4141 aatggcagca atgtggcaat gagccatgga aacaaagaag tattctcgtg ccgaggaata
     4201 aaattggcgg tggattggtt tttggaaaga ggccataaag atatcacggt ctttgttcct
     4261 gcttggagaa aagaacagtc ccgccctgac gccctcatca cagatcagga aattttacgt
     4321 aaactagaga aggaaaaaat cctggtattc acaccatcac ggcgtgtcca gggcaggcga
     4381 gtggtgtgct atgatgacag attcatcgtg aagctggctt ttgagtccga tggtataatt
     4441 gtgtccaatg ataactacag agatttggca aatgagaaac cagaatggaa gaagttcata
     4501 gatgagcggt tattgatgta ttcctttgtc aatgacaagt tcatgccccc agatgaccct
     4561 cttggcagac atggcccaag tctggataat tttctgagga agaaacccat tgttcctgag
     4621 cacaaaaagc agccttgtcc ctatggaaag aagtgtacgt atggacacaa gtgcaaatac
     4681 taccacccag aaaggggcag tcagcctcag cggtcagtgg ctgatgaact tcgtgccatg
     4741 tctagaaaca cagcagccaa aactacaaat gaagggggac tggtgaaaag caacagtgtt
     4801 ccttgcagca ccaaggcaga cagcacttcg gatgttaaac gcggtgctcc aaagaggcag
     4861 tcagacccaa gcataaggac tcacgtctac caagacatag aggagaagct tcccaccaaa
     4921 aacaagttgg aaaccaggtc tgtaccttcc ttagttagta tcccggccac ttctactgca
     4981 aaaccccaaa gcactacacc tttaagcaat ggccttccat ctggagttca tttcccacct
     5041 caggatcaaa gaccacaggg acaataccct ccaatgatga tggcaaccaa aaatcatgga
     5101 acgccaatgc cttatgaaca gtatccaaaa tgtgactccc ctgttgacgt tggatattat
     5161 tccatgttga atgcatattc taatctgagt atctcaggcc cccgaagtcc tgaaaggcgt
     5221 ttctcactag acactgatta cagagtaaac tctgtggctt cagactgcag cagtgaaggg
     5281 agcatgagtt gtgggagcag cgactcctac gtggggtaca atgaccgctc ctatgtcagc
     5341 tctcctgacc cacagctgga agagagtctc aagtgtcagc acatgcaccc ccacagccgc
     5401 cttaattccc agcccttcct gcagaatttc cacgatccct taaccagagt ccagagttac
     5461 agtcacgaag aaccaaagtt ccatcccaag cgtcctcttc cacacttggc tatgcatctg
     5521 cagcaccctg ccgtgggtgc ccgctccagc tgtcctggcg attacccctc cccaccaagt
     5581 tcagcacatt cgaaggcacc acacctaggg aggtccttag tggccacgag aatagacagc
     5641 atttctgatt cccgactcta tgatagttct ccttcaagac aaagaaagcc ttattctcgc
     5701 caggaaggcc taggaagctg gggtaggcca agctatgggc tggaagcata tggctatcgg
     5761 cagacgtact ccttacccga taattccacg cccccatgct atgagtcgat caccttccag
     5821 agcctgcctg agcagcagga acccacctgg agaatcccct actgtggaat gccacacgat
     5881 cctccaaggt accaggacaa ccgagagaag atttttatca atttgtgtaa catcttcccc
     5941 cctgaccttg tgaggctcgt catgaaaagg aatcctcata tgacggatgc ccagcagctc
     6001 gccgcagcca tcttagtgga gaaatcgcag ctgggttact gaggatccgt ttaaacgcgg
     6061 ccgcccagca cagtggctcg agccgcgggt taactggcca gaattctcga ccttgactgg
     6121 cggcgcgacc ttgaggcctg cgttcgcctc agttgccccc tctgtgcaat ggggagacgc
     6181 gcctcatcgc ttgacaacgg ccgaagagcc gccgcgcttc cgtctcccgc gtgcgcgcgc
     6241 catgctgccc acccccgttc cgcactgacc ctcccccgtg ccccgcgtcc cgtactgccg
     6301 ccccgccccg agtcccatgc cgcagccacc gcgacggagc ccgcaggcgg gaacctgcct
     6361 ccgcgcgtta gcgcgcacgc gcgcctcatg tgtcgtcccc atcagcgccg gcttccgtct
     6421 ataggccaga tgcactgtca ctctggcgaa gtcgcagacc cgattggccg ggacggaggc
     6481 gcgagaccgg gttgcgggcg gggccgaacg tggtataaaa ggggcgggag gccaggctcg
     6541 tgccgttttg cagacgccac cgccgaggaa aaccgtgtac tattagccac gcgtgccacc
     6601 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     6661 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     6721 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6781 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6841 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6901 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6961 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     7021 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     7081 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     7141 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     7201 gcgatcgcta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7261 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7321 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7381 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7441 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7501 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7561 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7621 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7681 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7741 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgctt
     7801 aatcgcgtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     7861 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     7921 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7981 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     8041 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     8101 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     8161 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     8221 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     8281 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     8341 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8401 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8461 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttcatg tgagcaaaag
     8521 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8581 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8641 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8761 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8881 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8941 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9001 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10321 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
    10381 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
    10441 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaag
//
