LOCUS       sequence-449836-        2756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for cloning gene of interest for in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Bataclan M, Leoni C, Moro SG, Pecoraro M, Wong EH, Heissmeyer V, 
            Monticelli S
  TITLE     Crosstalk between Regnase-1 and -3 shapes mast cell survival and 
            cytokine expression.
  JOURNAL   Life Sci Alliance. 2024 Jun 3;7(8):e202402784. doi: 
            10.26508/lsa.202402784. Print 2024 Aug.
  PUBMED    38830770
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2024-06-3"; volume: "7(8):e202402784. doi"; 
            pages: " 10.26508/lsa.202402784. Print 2024 Aug"
COMMENT     Sequence Label: pUC57 mini-ZsGreen
FEATURES             Location/Qualifiers
     source          1..2756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(371..390)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(372..390)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        430..534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(535..603,604..1395)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(753..772)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1566..2154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2055..2074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2378..2756,1..314))
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
ORIGIN
        1 cacacggcgc cgtcctcgaa caggaaggag cggtcccagg tgtagccggc ggggcaggag
       61 ttcttgaagt agtcgacgat gtcctggggg tactcggtga acacgcggtt gccgtacatg
      121 aaggcggcgg acaagatgtc ctcggcgaag ggcaaggggc cgccctccac cacgcacagg
      181 ttgatggcct gcttgccctt gaaggggtag ccgatgccct cgccggtgat cacgaacttg
      241 tggccgtcca cgcagccctc catgcggtac ttcatggtca tctccttggt caggccgtgc
      301 ttggactggg ccatggtgtc tgtttgaggt tgctagtgaa cacagttgtg tcagaagcaa
      361 atgtatcgat ccctatagtg agtcgtatta atcattggga cgtcaggtgg cacttttcgg
      421 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      481 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      541 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      601 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      661 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      721 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      781 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      841 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      901 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      961 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1021 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1081 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1141 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1201 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1261 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1321 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1381 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1441 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1501 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1561 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1621 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1681 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1741 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1801 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1861 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1921 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1981 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2041 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2101 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2161 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2221 ccgatactag tttgcaatga aaataaatgt tttttattag gcagaatcca gatgctcaag
     2281 gcccttcata atatccccca gtttagtagt tggacttagg gaacaaagga acctttaata
     2341 gaaattggac agcaagaaag cgagcctcga gttcgaaggg caaggcggag ccggaggcga
     2401 tggcgtgctc ggtcaggtgc cacttctggt tcttggcgtc gctgcggtcc tcgcgggtca
     2461 gcttgtgctg gatgaagtgc cagtcgggca tcttgcgggg cacggacttg gccttgtaca
     2521 cggtgtcgaa ctggcagcgc aagcggccac cgtccttcag cagcaggtac atgctcacgt
     2581 cgcccttcaa gatgccctgc ttgggcacgg ggatgatctt ctcgcaggag ggctcccagt
     2641 tgtcggtcat cttcttcatc acggggccgt cggcggggaa gttcacgccg tagaacttgg
     2701 actcgtggta catgcagttc tcctccacgc tcacggtgat gtcggcgttg cagatg
//
