LOCUS       sequence-449844-        7043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor ID2 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7043)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-ID2-Brd 57
FEATURES             Location/Qualifiers
     source          1..7043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    307..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(1391..1797)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    1831..1948
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2215..2259)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2677
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3170..3295
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3342..3522
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3523..3749)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(3777..3797)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3777..3795)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3816..3832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3816..3832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3829..3851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3840..3856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3864..3870,3871..3888,3889..3894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3909..3930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4047..4064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4218..4806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4298..4317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4977..5768,5769..5837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5600..5619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5838..5942)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5968..6423)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6105..6126)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6317..6336
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6550..6572
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6564..6581
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6565..6581
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6591..6610
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6591..6609
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6630..6765
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6805..6926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6816..6835)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6870..6889
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             join(6998..7043,1..188)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
       61 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
      121 taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgaattag
      181 cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt
      241 cattggtcta gaagcgctag acttgttgta catctagaag gtacagcttg atatcgaatt
      301 gcaaaggacg tcagcttccc cgacaacacc acggaattat cagtgcccag caacctagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttctg ccgtggcgat cgggaggggg
      421 aaagcgaaag tcccagaaag gagttgacag gtggtggcaa tgccccagcc agtgggggtt
      481 gcgtcagcaa acacagagca caccacgcca cgttgacgga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa cgaaagccgt acgggaagca
      601 atagcatgat acagaggcat taaagcagca tatccacaca gcgtaaaagg agcaacatag
      661 ttaagaatat cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt aacaggaaca
      721 gagcgtaaat aacgggaaag tttcttaaca tgtttgtctt gtggcaatac acctgaacta
      781 gtaattacat atccctaaaa atgtaaatga ttgccccacc attttgtttt attaacattt
      841 aaatgtatac ccaaatcaag aaaaacagaa caaatatggg aataaatggc ggtaagatgc
      901 tcgaattcct gcagcccggg ggatccgacc ggtctagcca cacagtgctt tgctgtcatt
      961 tgacattaac tcagaaggga attcagaagc ctgcaaggac aggatgctga tatccgtgtt
     1021 gagggtggtc agcggcgtcc tggacgcctg gttctgcccg ggtctctggt gatgcaggct
     1081 gacaatagtg ggatgcgagt ccagggcgat ctgcaggtcc aagatgtagt cgatgacgtg
     1141 ctgcaggatt tccatcttgc tcaccttctt gttctggggg atgctgggca ccagctcctt
     1201 gagcttggag tagcagtcgt tcatgttgta tagcaggctc atcgggtcgt ccacaggggt
     1261 tttgctccgg gagatgccca ggctgtggtc cgacaggctg tttttcctaa cggacctcac
     1321 gggactgaag gctttcattt cgaaccgcgg gctagcggat ccgtcgacgg tatcgataag
     1381 cttgattcga ccggccgact cagtctgtcg gaggactggc gcgccgagtg aggggttgtg
     1441 agctctttta tagagctcgg gaagcagaag cgcgcgaaca gaagcgagaa gcaggctgat
     1501 tggttaattc aaataaggcg cagggtcatt tcaggtcctt gggggagcct ggaaacatct
     1561 gatgggtctt aagaaactgc tgagggttgg gccatatctg gggaccatct gttcttggcc
     1621 tcgggccggg gccgaaactg cggtgaccat ctgttcttgg ccccgggccg gggccgaaac
     1681 tgctcaccgc agatatcctg tttggcccaa cgttagctat tttcatgtac ccgcccttga
     1741 tctgaacttc tctattcttg gtttggtatt tttccatgcc ttgcaaaatg gcgttactgc
     1801 agccagctcg aggctagtct cgtgatcgat aaaattttga atttttgtaa tttgtttttg
     1861 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     1921 ccccccaatc cccccttttc ttttaaaagt taaccgatac cgtcgagatc cgttcactaa
     1981 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc
     2041 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
     2101 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
     2161 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
     2221 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
     2281 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
     2341 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
     2401 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
     2461 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
     2521 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
     2581 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
     2641 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
     2701 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
     2761 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa
     2821 gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
     2881 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
     2941 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
     3001 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
     3061 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     3121 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     3181 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     3241 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     3301 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     3361 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     3421 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     3481 agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag
     3541 gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag
     3601 acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca
     3661 tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca
     3721 agactacaag agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt
     3781 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3841 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3901 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3961 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4021 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4081 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4621 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4981 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5041 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5101 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5161 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5221 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5281 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5341 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5401 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5461 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5521 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5581 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5641 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5701 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5761 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5821 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5881 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5941 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     6001 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     6061 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     6121 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     6181 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     6241 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6301 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6361 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6421 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6481 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     6541 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     6601 cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg
     6661 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     6721 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     6781 cgggatagct agagccagac atgataagat acattgatga gtttggacaa accacaacta
     6841 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     6901 ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt
     6961 tataaatact gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac
     7021 taaaagggtc tgagggatct cta
//
