LOCUS       sequence-449845-        9548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor NFKB1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9548)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 9548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-NFKB1-Brd 61
FEATURES             Location/Qualifiers
     source          1..9548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    361..764
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             883..1116
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1188..1309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1225..1244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1279..1298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1349..1484
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1504..1523)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1505..1523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1533..1550)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1533..1549)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1542..1564)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1691..2146
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1778..1797)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1988..2009
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2172..2276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2277..2345,2346..3137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2495..2514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3308..3896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3797..3816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4050..4067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4184..4205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4220..4225,4226..4243,4244..4250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4258..4274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4263..4285
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4282..4298
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4282..4298
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4317..4337
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4319..4337
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4365..4591
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4592..4772
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4819..4944
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5437..5670
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5855..5899
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6166..6283
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6317..6723
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 tctgcgacag cggcgtggag acatccttcc gcaaactcag ctttaccgag tctctgacca
       61 gtggtgcctc actgctaact ctcaacaaaa tgccccatga ttatgggcag gaaggacctc
      121 tagaaggcaa aatttagacc ggtcggatcc cccgggctgc aggaattcga gcatcttacc
      181 gccatttatt cccatatttg ttctgttttt cttgatttgg gtatacattt aaatgttaat
      241 aaaacaaaat ggtggggcaa tcatttacat ttttagggat atgtaattac tagttcaggt
      301 gtattgccac aagacaaaca tgttaagaaa ctttcccgtt atttacgctc tgttcctgtt
      361 aatcaacctc tggattacaa aatttgtgaa agattgactg atattcttaa ctatgttgct
      421 ccttttacgc tgtgtggata tgctgcttta atgcctctgt atcatgctat tgcttcccgt
      481 acggctttcg ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
      541 tggcccgttg tccgtcaacg tggcgtggtg tgctctgtgt ttgctgacgc aacccccact
      601 ggctggggca ttgccaccac ctgtcaactc ctttctggga ctttcgcttt ccccctcccg
      661 atcgccacgg cagaactcat cgccgcctgc cttgcccgct gctggacagg ggctaggttg
      721 ctgggcactg ataattccgt ggtgttgtcg gggaagctga cgtcctttgc aattcgatat
      781 caagctgtac cttctagatg tacacgtctt atagcgcttc tagaccaatg acttacaagg
      841 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
      901 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
      961 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1021 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1081 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     1141 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     1201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     1321 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1381 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     1441 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     1501 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     1561 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     1621 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1681 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1741 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     1801 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     1861 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     1921 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     1981 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2041 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2101 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     2161 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2221 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2281 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2341 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2401 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2461 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2521 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2581 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2641 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2701 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2761 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2821 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2881 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2941 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3001 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3061 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3121 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3241 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3301 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3901 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3961 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4021 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4141 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     4201 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     4261 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     4321 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     4381 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     4441 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     4501 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     4561 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     4621 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4681 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4741 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     4801 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     4861 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     4921 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     4981 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     5041 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     5101 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     5161 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     5221 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     5281 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     5341 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     5401 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     5461 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     5521 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     5581 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     5641 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     5701 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     5761 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     5821 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     5881 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     5941 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     6001 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     6061 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     6121 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
     6181 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     6241 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc acgagactag
     6301 cctcgagctg gctgcagtaa cgccattttg caaggcatgg aaaaatacca aaccaagaat
     6361 agagaagttc agatcaaggg cgggtacatg aaaatagcta acgttgggcc aaacaggata
     6421 tctgcggtga gcagtttcgg ccccggcccg gggccaagaa cagatggtca ccgcagtttc
     6481 ggccccggcc cgaggccaag aacagatggt ccccagatat ggcccaaccc tcagcagttt
     6541 cttaagaccc atcagatgtt tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta
     6601 tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct
     6661 ctataaaaga gctcacaacc cctcactcgg cgcgccagtc ctccgacaga ctgagtcggc
     6721 cggtcgaatc aagcttatcg ataccgtcga cggatccgct agcccgcggt tcgaaatggc
     6781 agaagatgat ccatatttgg gaaggcctga acaaatgttt catttggatc cttctttgac
     6841 tcatacaata tttaatccag aagtatttca accacagatg gcactgccaa cagcagatgg
     6901 cccatacctt caaatattag agcaacctaa acagagagga tttcgtttcc gttatgtatg
     6961 tgaaggccca tcccatggtg gactacctgg tgcctctagt gaaaagaaca agaagtctta
     7021 ccctcaggtc aaaatctgca actatgtggg accagcaaag gttattgttc agttggtcac
     7081 aaatggaaaa aatatccacc tgcatgccca cagcctggtg ggaaaacact gtgaggatgg
     7141 gatctgcact gtaactgctg gacccaagga catggtggtc ggcttcgcaa acctgggtat
     7201 acttcatgtg acaaagaaaa aagtatttga aacactggaa gcacgaatga cagaggcgtg
     7261 tataaggggc tataatcctg gactcttggt gcaccctgac cttgcctatt tgcaagcaga
     7321 aggtggaggg gaccggcagc tgggagatcg ggaaaaagag ctaatccgcc aagcagctct
     7381 gcagcagacc aaggagatgg acctcagcgt ggtgcggctc atgtttacag cttttcttcc
     7441 ggatagcact ggcagcttca caaggcgcct ggaacccgtg gtatcagacg ccatctatga
     7501 cagtaaagcc cccaatgcat ccaacttgaa aattgtaaga atggacagga cagctggatg
     7561 tgtgactgga ggggaggaaa tttatcttct ttgtgacaaa gttcagaaag atgacatcca
     7621 gattcgattt tatgaagagg aagaaaatgg tggagtctgg gaaggatttg gagatttttc
     7681 ccccacagat gttcatagac aatttgccat tgtcttcaaa actccaaagt ataaagatat
     7741 taatattaca aaaccagcct ctgtgtttgt ccagcttcgg aggaaatctg acttggaaac
     7801 tagtgaacca aaacctttcc tctactatcc tgaaatcaaa gataaagaag aagtgcagag
     7861 gaaacgtcag aagctcatgc ccaatttttc ggatagtttc ggcggtggta gtggtgccgg
     7921 agctggaggc ggaggcatgt ttggtagtgg cggtggagga gggggcactg gaagtacagg
     7981 tccagggtat agcttcccac actatggatt tcctacttat ggtgggatta ctttccatcc
     8041 tggaactact aaatctaatg ctgggatgaa gcatggaacc atggacactg aatctaaaaa
     8101 ggaccctgaa ggttgtgaca aaagtgatga caaaaacact gtaaacctct ttgggaaagt
     8161 tattgaaacc acagagcaag atcaggagcc cagcgaggcc accgttggga atggtgaggt
     8221 cactctaacg tatgcaacag gaacaaaaga agagagtgct ggagttcagg ataacctctt
     8281 tctagagaag gctatgcagc ttgcaaagag gcatgccaat gcccttttcg actacgcggt
     8341 gacaggagac gtgaagatgc tgctggccgt ccagcgccat ctcactgctg tgcaggatga
     8401 gaatggggac agtgtcttac acttagcaat catccacctt cattctcaac ttgtgaggga
     8461 tctactagaa gtcacatctg gtttgatttc tgatgacatt atcaacatga gaaatgatct
     8521 gtaccagacg cccttgcact tggcagtgat cactaagcag gaagatgtgg tggaggattt
     8581 gctgagggct ggggccgacc tgagccttct ggaccgcttg ggtaactctg ttttgcacct
     8641 agctgccaaa gaaggacatg ataaagttct cagtatctta ctcaagcaca aaaaggcagc
     8701 actacttctt gaccacccca acggggacgg tctgaatgcc attcatctag ccatgatgag
     8761 caatagcctg ccatgtttgc tgctgctggt ggccgctggg gctgacgtca atgctcagga
     8821 gcagaagtcc gggcgcacag cactgcacct ggctgtggag cacgacaaca tctcattggc
     8881 aggctgcctg ctcctggagg gtgatgccca tgtggacagt actacctacg atggaaccac
     8941 acccctgcat atagcagctg ggagagggtc caccaggctg gcagctcttc tcaaagcagc
     9001 aggagcagat cccctggtgg agaactttga gcctctctat gacctggatg actcttggga
     9061 aaatgcagga gaggatgaag gagttgtgcc tggaaccacg cctctagata tggccaccag
     9121 ctggcaggta tttgacatat taaatgggaa accatatgag ccagagttta catctgatga
     9181 tttactagca caaggagaca tgaaacagct ggctgaagat gtgaagctgc agctgtataa
     9241 gttactagaa attcctgatc cagacaaaaa ctgggctact ctggcgcaga aattaggtct
     9301 ggggatactt aataatgcct tccggctgag tcctgctcct tccaaaacac ttatggacaa
     9361 ctatgaggtc tctgggggta cagtcagaga gctggtggag gccctgagac aaatgggcta
     9421 caccgaagca attgaagtga tccaggcagc ctccagccca gtgaagacca cctctcaggc
     9481 ccactcgctg cctctctcgc ctgcctccac aaggcagcaa atagacgagc tccgagacag
     9541 tgacagtg
//
