LOCUS       sequence-44988-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(1057-1234)
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..105
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(699..717)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      713..760
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgngaattcc ctctgaatat tttgtttact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat gagtattaat
      121 gaaataggtt ccagtgatga aaacattcaa gcagaactag gtagaaacag agggccaaaa
      181 ttgaatgcta tgcttagatt aggggttttg caacctgagg tctataaaca aagtcttcct
      241 ggaagtaatt gtaagcatcc tgaaataaaa aagcaagaat atgaagaagt agttcagact
      301 gttaatacag atttctctcc atatctgatt tcagataact tagaacagcc tatgggaagt
      361 agtcatgcat ctcaggtttg ttctgagaca cctgatgacc tgttagatga tggtgaaata
      421 aaggaagata ctagttttgc tgaaaatgac attaaggaaa gttctgctgt ttttagcaaa
      481 agcgtccaga aaggagagct tagcaggagt cctagccctt tcacccatac acatttggct
      541 cagggttacc gaagaggggc caagaaatta gagtcctcag aagagaactt atctagtgag
      601 gatgaagagc ttccctgctt ccaacacttg ttatttggta aagtataagg atccggctgc
      661 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      721 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaac
//
