LOCUS       sequence-449898-        8138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor RUNX2 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8138)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-RUNX2-Brd 73
FEATURES             Location/Qualifiers
     source          1..8138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    61..186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(414..640)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(668..688)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(707..723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(707..723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(720..742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    731..747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(755..761,762..779,780..785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    800..821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(938..955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1109..1697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1189..1208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1868..2659,2660..2728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2491..2510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2729..2833)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2859..3314)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2996..3017)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3208..3227
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3441..3463
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3455..3472
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3456..3472
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3482..3501
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3482..3500
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3521..3656
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3696..3817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3707..3726)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3761..3780
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3889..4122
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4241..4644
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(6420..6826)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6860..6977
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7244..7288)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7473..7706
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
       61 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
      121 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
      181 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
      241 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
      301 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
      361 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg
      421 tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca
      481 atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc
      541 accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt
      601 accatgttgc aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct
      661 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
      721 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      901 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      961 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1021 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1141 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1621 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1741 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1801 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1861 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1921 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1981 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2041 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2101 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2161 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2221 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2281 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2341 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2401 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2461 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2521 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2581 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2641 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2701 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2821 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     2881 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     2941 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3001 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3061 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3121 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3181 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3241 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3301 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     3361 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     3421 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
     3481 gtaatacgac tcactatagg gcgaattggg tacgtcccta ggcctccaaa aaagcctcct
     3541 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     3601 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     3661 ggagttaggg gcgggatagc tagagccaga catgataaga tacattgatg agtttggaca
     3721 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     3781 tttatttgta accattataa gctgcaataa acaagttcct ctcactctct gatattcatt
     3841 tctttgcaag ttataaatac tgaataataa gatgacatga actactactg ctagagattt
     3901 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     3961 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     4021 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa
     4081 aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta
     4141 aaaagtggct aagatctaca gctgccttgt aagtcattgg tctagaagcg ctcaaaatgg
     4201 tgtacatcta gaaggtacag cttgatatcg aattgcaaag gacgtcagct tccccgacaa
     4261 caccacggaa ttatcagtgc ccagcaacct agcccctgtc cagcagcggg caaggcaggc
     4321 ggcgatgagt tctgccgtgg cgatcgggag ggggaaagcg aaagtcccag aaaggagttg
     4381 acaggtggtg gcaatgcccc agccagtggg ggttgcgtca gcaaacacag agcacaccac
     4441 gccacgttga cggacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata
     4501 caaggaggag aaaacgaaag ccgtacggga agcaatagca tgatacagag gcattaaagc
     4561 agcatatcca cacagcgtaa aaggagcaac atagttaaga atatcagtca atctttcaca
     4621 aattttgtaa tccagaggtt gattaacagg aacagagcgt aaataacggg aaagtttctt
     4681 aacatgtttg tcttgtggca atacacctga actagtaatt acatatccct aaaaatgtaa
     4741 atgattgccc caccattttg ttttattaac atttaaatgt atacccaaat caagaaaaac
     4801 agaacaaata tgggaataaa tggcggtaag atgctcgaat tcctgcagcc cgggggatcc
     4861 gaccggtcta atatggtcgc caaacagatt catccattct gccactagaa ttcaaaacag
     4921 ttggggaact gctgtggctt ccatcagcgt caacaccatc attctggtta ggcaaatttg
     4981 gatttaatag cgtgctgcca ttcgaggtgg tggtgcatgg cggaagcatt ctggaaggag
     5041 accggtctcc ccccggcacc atgggaaact gataggatcc tgacgaagtg ccatagtaga
     5101 gatatggagt gctgctggtc tggaagggtc cactctggct ttgggaagag ccggggtagg
     5161 gtggtggcag gtaggtgtgg tagtgagtgg tggcggacat accgagggac atgcctgagg
     5221 tgactggcgg ggtgtaagta aaggtggctg gatagtgcat tcgtgggttg gagaagcggc
     5281 tctcagtgag ggatgaaatg cttgggaact gcctggggtc tgaaaaaggg cccagttctg
     5341 aagcacctga aatgcgccta ggcacatcgg tgatggcagg aagcccagtg ccccgtgtgg
     5401 aagacagcgg ggtggtagag tggatggacg gggacgtcat ctggctcagg taggaggggt
     5461 aagactggtc ataggaccac ggcggggaag actgtgcctg cctggggtct gtaatctgac
     5521 tctgtccttg tggattaaaa ggacttggtg cagagttcag ggagggccgt gggttctgag
     5581 gcgggacacc tactctcata ctgggatgag gaatgcgccc taaatcactg aggcggtcag
     5641 agaacaaact aggtttagag tcatcaagct tctgtctgtg ccttctgggt tcccgaggtc
     5701 catctactgt aactttaatt gctctgtgat aggtagctac ttggggagga tttatgaaga
     5761 cggttatggt caaggtgaaa ctcttgcctc gtccactccg gcccacaaat ctcagatcgt
     5821 tgaaccttgc tacttggttt ttcataacag cagaggcatt ccggagctca gcagaataat
     5881 tttcatcgtt acccgccatg acagtaacca cagtcccatc tggtacctct ccgagggcta
     5941 ccaccttgaa ggccacgggc agggtcttgt tgcagcgcca gtgcgagggc agcaccgagc
     6001 acaggaagtt ggggctgtcg gtgcggacga gttcggccgg gtggtcggcg atgatctcca
     6061 ccatggtgcg gttgtcgtgg ggcggccgca accggggcac tgcagctgcc gccgccgcag
     6121 ccgccgccgc cgccgcagcc gccgccgccg cctcctgctg ctgctgctgc tgttgctgct
     6181 gctgctgctg ctgttgctgc tgctgctgtt gctgctgctg ttgcgcagcc accaccgggc
     6241 tcacgtcgct cattttgccg ggctgcaggc tgctggaggg ggggctgaag cgccggctgg
     6301 tgctcggatc ccaaaagaag ttttgctgac atggtgtcac tgtgctgaag aggctgtttg
     6361 atgccatttc gaaccgcggg ctagcggatc cgtcgacggt atcgataagc ttgattcgac
     6421 cggccgactc agtctgtcgg aggactggcg cgccgagtga ggggttgtga gctcttttat
     6481 agagctcggg aagcagaagc gcgcgaacag aagcgagaag caggctgatt ggttaattca
     6541 aataaggcgc agggtcattt caggtccttg ggggagcctg gaaacatctg atgggtctta
     6601 agaaactgct gagggttggg ccatatctgg ggaccatctg ttcttggcct cgggccgggg
     6661 ccgaaactgc ggtgaccatc tgttcttggc cccgggccgg ggccgaaact gctcaccgca
     6721 gatatcctgt ttggcccaac gttagctatt ttcatgtacc cgcccttgat ctgaacttct
     6781 ctattcttgg tttggtattt ttccatgcct tgcaaaatgg cgttactgca gccagctcga
     6841 ggctagtctc gtgatcgata aaattttgaa tttttgtaat ttgtttttgt aattctttag
     6901 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
     6961 ccccttttct tttaaaagtt aaccgatacc gtcgagatcc gttcactaat cgaatggatc
     7021 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
     7081 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
     7141 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
     7201 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
     7261 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
     7321 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
     7381 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
     7441 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
     7501 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
     7561 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
     7621 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
     7681 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
     7741 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
     7801 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
     7861 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     7921 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     7981 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     8041 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
     8101 cttgcccata ctatatgttt taatttatat tttttctt
//
