LOCUS       sequence-449899-        7391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor RUNX1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7391)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-RUNX1-Brd 75
FEATURES             Location/Qualifiers
     source          1..7391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    110..231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(147..166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     201..220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      271..406
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(426..445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(427..445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(455..472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(455..471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(464..486)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      613..1068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(700..719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     910..931
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1094..1198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1199..1267,1268..2059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1417..1436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2230..2818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2719..2738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2972..2989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3106..3127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3142..3147,3148..3165,3166..3172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3180..3196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3185..3207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3204..3220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3204..3220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3239..3259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3241..3259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3287..3513
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3514..3694
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3741..3866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4359..4592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4777..4821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5088..5205
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5239..5645
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6674..7077
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             join(7196..7391,1..38)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
       61 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
      121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
      241 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
      301 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
      361 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
      421 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
      481 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      541 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      601 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      661 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      721 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      781 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      841 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      901 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
      961 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1021 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     1081 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1141 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1201 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1261 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1321 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1381 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1441 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1501 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1561 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1621 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1681 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1741 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1801 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1861 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1921 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1981 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2041 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2101 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2161 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2221 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2281 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2341 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2401 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2461 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2521 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2581 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2641 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2701 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2761 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2821 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2881 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2941 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3001 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3061 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     3121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     3241 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     3301 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     3361 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     3421 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     3481 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     3541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     3721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     3781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     3841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     3901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     3961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     4021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     4081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     4141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     4201 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     4261 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     4321 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     4381 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     4441 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     4501 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     4561 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     4621 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     4681 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     4741 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     4801 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     4861 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     4921 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     4981 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     5041 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     5101 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     5161 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
     5221 agcctcgagc tggctgcagt aacgccattt tgcaaggcat ggaaaaatac caaaccaaga
     5281 atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg ccaaacagga
     5341 tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt
     5401 tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac cctcagcagt
     5461 ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct
     5521 tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag
     5581 ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca gactgagtcg
     5641 gccggtcgaa tcaagcttat cgataccgtc gacggatccg ctagcccgcg gttcgaaatg
     5701 cgtatccccg tagatgccag cacgagccgc cgcttcacgc cgccttccac cgcgctgagc
     5761 ccaggcaaga tgagcgaggc gttgccgctg ggcgccccgg acgccggcgc tgccctggcc
     5821 ggcaagctga ggagcggcga ccgcagcatg gtggaggtgc tggccgacca cccgggcgag
     5881 ctggtgcgca ccgacagccc caacttcctc tgctccgtgc tgcctacgca ctggcgctgc
     5941 aacaagaccc tgcccatcgc tttcaaggtg gtggccctag gggatgttcc agatggcact
     6001 ctggtcactg tgatggctgg caatgatgaa aactactcgg ctgagctgag aaatgctacc
     6061 gcagccatga agaaccaggt tgcaagattt aatgacctca ggtttgtcgg tcgaagtgga
     6121 agagggaaaa gcttcactct gaccatcact gtcttcacaa acccaccgca agtcgccacc
     6181 taccacagag ccatcaaaat cacagtggat gggccccgag aacctcgaag acatcggcag
     6241 aaactagatg atcagaccaa gcccgggagc ttgtcctttt ccgagcggct cagtgaactg
     6301 gagcagctgc ggcgcacagc catgagggtc agcccacacc acccagcccc cacgcccaac
     6361 cctcgtgcct ccctgaacca ctccactgcc tttaaccctc agcctcagag tcagatgcag
     6421 gaggaagaca cagcaccctg gagatgttag accggtcgga tcccccgggc tgcaggaatt
     6481 cgagcatctt accgccattt attcccatat ttgttctgtt tttcttgatt tgggtataca
     6541 tttaaatgtt aataaaacaa aatggtgggg caatcattta catttttagg gatatgtaat
     6601 tactagttca ggtgtattgc cacaagacaa acatgttaag aaactttccc gttatttacg
     6661 ctctgttcct gttaatcaac ctctggatta caaaatttgt gaaagattga ctgatattct
     6721 taactatgtt gctcctttta cgctgtgtgg atatgctgct ttaatgcctc tgtatcatgc
     6781 tattgcttcc cgtacggctt tcgttttctc ctccttgtat aaatcctggt tgctgtctct
     6841 ttatgaggag ttgtggcccg ttgtccgtca acgtggcgtg gtgtgctctg tgtttgctga
     6901 cgcaaccccc actggctggg gcattgccac cacctgtcaa ctcctttctg ggactttcgc
     6961 tttccccctc ccgatcgcca cggcagaact catcgccgcc tgccttgccc gctgctggac
     7021 aggggctagg ttgctgggca ctgataattc cgtggtgttg tcggggaagc tgacgtcctt
     7081 tgcaattcga tatcaagctg taccttctag atgtacaatc taacaagcgc ttctagacca
     7141 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7201 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     7261 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7321 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7381 aactagagat c
//
