LOCUS       sequence-449903-        9161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, 
            Wan J, Fang D, Bintu L
  TITLE     The H3.3 K36M oncohistone disrupts the establishment of epigenetic 
            memory through loss of DNA methylation.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147.
  PUBMED    37873347
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147."
COMMENT     Sequence Label: pLenti-rTetR(SE)-HP1a-2A-mTurquoise
FEATURES             Location/Qualifiers
     source          1..9161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2060..2080)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(2099..2809,2810..2812,2813..2815))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2141..2162)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2490..2509
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2749..2770
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2816..2869)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3458..3523)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4164..5102
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4187..4207)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(5103..5314)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    5437..5554
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5852..5896)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6081..6314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6811..6936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6983..7163
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7181..7379)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7206..7226)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7381..7760
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7932..7951
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8026..8130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8131..8199,8200..8991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8349..8368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcttat acctttctct tcttttttgg gctaccgctg ccgctaccct
     2101 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     2161 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt
     2221 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     2281 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
     2341 tctgcttgtc ggcggtgata tagacgttgt cgctgatgta gttgtactcc agcttgtgcc
     2401 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     2461 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     2521 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     2581 tgtggtcggg gtagcgggcg aagcactgca cgccccagga cagggtggtc acgagggtgg
     2641 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     2701 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     2761 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accattgggc
     2821 caggattctc ctcgacatct ccgcaagtta ggaggcttcc ccttccctct ccgctctttg
     2881 ctgtttcttt ctctttgttt tccgcatcct caggatatgc atgccatgtc agtctctctt
     2941 cataaaatgc tatcacaatt tgtggacatt tcacattagc ttcttttgca agaaccaggt
     3001 cagcttcatc tgtgtctttc catttcatta ggaacattaa atcaccacag gaatctgttg
     3061 ccccaatgat cttttctggt tccagtcctc tctcaaagcc ccgagcgata tcattgctct
     3121 gctctctctt ttttttagat ttgatgtcat cggcactgtt tgagaaattg gatttcctct
     3181 tgttactttc tgacttctcc ctgggtttat tattttcacc ctccttcatc ttcttatact
     3241 ttttcataaa ttcagaaatt agctcagggc aatccaagtt tttctcaggt tcccaagtat
     3301 tgtgctcctc agaaaagcct ttccacttca gtagatattc cacttgtccc ttaaccacgc
     3361 gcctgtctag caccttctcc acgacatact cctcctcatc ctctgaagaa gaactgtcag
     3421 ctgtccgctt ggttttcttt cctgatccgg atccaccctt gtcatcgtca tccttgtagt
     3481 cgatgtcatg atctttataa tcaccgtcat ggtctttgta gtcaccgccg ctttcgcact
     3541 ttagctgttt ctccaggcca catatgatta gttccaggcc gaaaaggaag gcaggttcgg
     3601 ctccctgccg gtcgaacagc tcaattgctt gtttcagaag tgggggcata gaatcggtgg
     3661 taggtgtctc tctttcctct tttgctactt gatgctcctg ttcctccaat acgcagccca
     3721 gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt ctccagggag aagccttgct
     3781 gacacaggaa cgcgagctga ttttccaggg tttcgtactg tttctctgtt gggcgggtgc
     3841 cgagatgcac tttagccccg tcgcgatgtg agaggagagc acagcggtat gacttggcgt
     3901 tgttccgcag aaagtcttgc catgactcgg gttccagggg gcaggagtgg gtatgatgcc
     3961 tgtccagcat ctcgattggc agggcatcga gcagggcccg cttgttcttc acgtgccagt
     4021 acagggtagg ctgctcaact cccagctttt gagcgagttt ccttgtcgtc aggccttcga
     4081 taccgacacc attgagtaat tccagagctc cgtttattat tttgctcttg tccagtctag
     4141 acatggtggc tagctcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga
     4201 ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc
     4261 aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca
     4321 taaaacccct ccccccaacc taaagacgac gtactccaaa agcgcgagaa ctaatcgagg
     4381 tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag
     4441 gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat
     4501 tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc
     4561 gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc
     4621 agagcaggcc ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg
     4681 ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca
     4741 aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa
     4801 aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg
     4861 agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc
     4921 tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca
     4981 cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa
     5041 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
     5101 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
     5161 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     5221 cagtttaccc cgcgccacct tctctaggca ccggttcaat tgccgacccc tccccccaac
     5281 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcacatgc atggcggtaa
     5341 tacggttatc cacgcggccg cctgagtcaa atgggatctc tagagcgggc ccggtactaa
     5401 ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
     5461 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
     5521 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct
     5581 gcagaattgg cgcacgcagt gccgatccgt tcactaatcg aatggatctg tctctgtctc
     5641 tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt
     5701 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag
     5761 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc
     5821 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata
     5881 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc
     5941 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt
     6001 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc
     6061 cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga
     6121 ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct
     6181 caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct
     6241 gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa
     6301 ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct
     6361 tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc
     6421 tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgg ccgcttgctg
     6481 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     6541 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     6601 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     6661 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     6721 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     6781 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     6841 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     6901 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     6961 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     7021 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     7081 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     7141 atctggtcta accagagaga cccagtacag gcaaaacgcg ctgcttatat agacctccca
     7201 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     7261 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     7321 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     7381 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     7441 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     7501 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     7561 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     7621 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     7681 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     7741 actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa
     7801 acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc
     7861 ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg
     7921 gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga
     7981 tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8041 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8101 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8341 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8641 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
