LOCUS       sequence-449905-       10028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of rTetR(SE) fused to the catalytic domain of 
            DNMT3B and mTurquoise-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, 
            Wan J, Fang D, Bintu L
  TITLE     The H3.3 K36M oncohistone disrupts the establishment of epigenetic 
            memory through loss of DNA methylation.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147.
  PUBMED    37873347
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147."
COMMENT     Sequence Label: pLenti-rTetR(SE)-DNMT3Bcd-2A-mTurquoise
FEATURES             Location/Qualifiers
     source          1..10028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2060..2080)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(2099..2809,2810..2812,2813..2815))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2141..2162)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2490..2509
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2749..2770
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2816..2869)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4325..4390)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          5031..5969
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5054..5074)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(5970..6181)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    6304..6421
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6719..6763)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6948..7181
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7678..7803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7850..8030
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8048..8246)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8073..8093)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8248..8627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8799..8818
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8893..8997
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8998..9066,9067..9858)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9216..9235)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcttat acctttctct tcttttttgg gctaccgctg ccgctaccct
     2101 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     2161 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt
     2221 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     2281 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
     2341 tctgcttgtc ggcggtgata tagacgttgt cgctgatgta gttgtactcc agcttgtgcc
     2401 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     2461 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     2521 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     2581 tgtggtcggg gtagcgggcg aagcactgca cgccccagga cagggtggtc acgagggtgg
     2641 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     2701 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     2761 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accattgggc
     2821 caggattctc ctcgacatct ccgcaagtta ggaggcttcc ccttccctct ccttcacagg
     2881 caaagtagtc cttcaagggg gcaaacaggt gtctgatgac cggtacactc caggacctgc
     2941 ccagcagctt ctgacgggcg ccgcggccca tgttggacac gtccgtgtag tgagcaggga
     3001 agccgaagat cctttcgagc tcagtgcacc acaaaacgtc gtccttgcca ttcatgacta
     3061 cagggaaaag ctggtttttg ccctgtctga tggagttcga cttggtggtt attgtctgca
     3121 ctttctttaa ctttgctgtc ctactgaact ccaggcagtc ctgcagctcg agcttatcat
     3181 tctttgaagc catcacgggc ctgttcattc cgggtaggtt accccagaag taccgggccc
     3241 tgtgagcagc agacaccttg atggcatcga tcatcactgg gttacatgcc aggaatcttg
     3301 agatgtcttt cttgtcattc actttcatgg ccacaacatt ctcgaacatc cagaagaatg
     3361 gacggttgtc gccctccttg gggcgggtat aattcagcaa gtggtaaaac tcgaagaaga
     3421 gccttcctgt gccctcatat aaacctttgc gggcaggatt gacgttagag agatcattgc
     3481 atgggcttcc accaatcacc aagtcgaacg ggccccactc ttcaatattt ttcttggtga
     3541 ttttccggac gtcattgaca tatttgatct ggccttcatg cttaacagtt cccacagcga
     3601 tggactctgc acagacttcg gaggcaatgt acttttccac tttaataccc aactccttga
     3661 gcaccaagta ccccgttgca attccatcaa acagagacag gactctaatg ggcctccttt
     3721 tggctgcagg aattgctggg tacaacttgg gtggctcctg aaattcttcc aggtcaggat
     3781 cagtagtgaa gaagtcttgc aggcgcatgt tccaatcttt cctgcgtcgg aggaccccat
     3841 ggcagcgctg agggaggcac atatagcagc tccagggttc ctgcagcttg gcatcctcag
     3901 ctgtgcctgc gcccaccagc acctccagac actccacaca gaagcatctg cagcagcttg
     3961 tgttactgca cagcagcagt tcacggccct cacagcacac ggtgcagtag gactgatagc
     4021 cgtcctcatc atacatgtag aagagctcta ggaagcgatc ccggcaactc tgacagagcc
     4081 caccctcaaa gagggggtgg aaggacacag ggttcttctt tccacaggac aaacagcggt
     4141 cctccagatt gcccttgttg ttggtgactt cagaagccat ccgttctcgg ctctcctcat
     4201 cctcccctcg gtccttcccg ccatagctat ttgtcttgag gcgcttgggt gggggggact
     4261 cagaagcagc agagtcattg gttgtgcatg cgctaccgct gccgctacct gatccggatc
     4321 cacccttgtc atcgtcatcc ttgtagtcga tgtcatgatc tttataatca ccgtcatggt
     4381 ctttgtagtc accgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt
     4441 ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtt
     4501 tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat
     4561 gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca
     4621 gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt
     4681 cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg cgatgtgaga
     4741 ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat gactcgggtt
     4801 ccagggggca ggagtgggta tgatgcctgt ccagcatctc gattggcagg gcatcgagca
     4861 gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc agcttttgag
     4921 cgagtttcct tgtcgtcagg ccttcgatac cgacaccatt gagtaattcc agagctccgt
     4981 ttattatttt gctcttgtcc agtctagaca tggtggctag ctcacgacac ctgaaatgga
     5041 agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa
     5101 agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc
     5161 cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa agacgacgta
     5221 ctccaaaagc gcgagaacta atcgaggtgc ctggacggcg cccggtactc cgtggagtca
     5281 catgaagcga cggctgagga cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc
     5341 cgctctcccg agcgccgcgt cctccatttt gagctccctg cagcagggcc gggaagcggc
     5401 catctttccg ctcacgcaac tggtgccgac cgggccagcc ttgccgccca gggcggggcg
     5461 atacacggcg gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga ctacccccgt
     5521 ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca
     5581 cgggccccgt cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag atcttggccc
     5641 gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa
     5701 atggctagag acttatcgaa agcagcgaga caggcgcgaa ggtgccacca gattcgcacg
     5761 cggcggcccc agcgcccagg ccaggcctca actcaagcac gaggcgaagg ggctccttaa
     5821 gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa gctcgggatc aagaatcacg
     5881 tactgcagcc aggtggaagt aattcaaggc acgcaagggc cataacccgt aaagaggcca
     5941 ggcccgcggg aaccacacac ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa
     6001 agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg
     6061 cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg
     6121 gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc
     6181 cactgacggg cacatgcatg gcggtaatac ggttatccac gcggccgcct gagtcaaatg
     6241 ggatctctag agcgggcccg gtactaacca aactggatct ctgctgtccc tgtaataaac
     6301 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     6361 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     6421 attgtggatg aatactgcca tttgtctgca gaattggcgc acgcagtgcc gatccgttca
     6481 ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc
     6541 tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc
     6601 taactctatt cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta
     6661 ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca
     6721 aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg
     6781 attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca
     6841 tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag
     6901 gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat
     6961 cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg
     7021 tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca
     7081 ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag
     7141 tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt
     7201 ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta
     7261 ctactttata tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc
     7321 tgaagatcag cggccggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc
     7381 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag
     7441 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg
     7501 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc
     7561 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc
     7621 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac
     7681 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac
     7741 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga
     7801 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt
     7861 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     7921 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     7981 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca
     8041 aaacgcgctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
     8101 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     8161 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     8221 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
     8281 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     8341 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     8401 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     8461 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     8521 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     8581 tgaccccgta attgattact attaataact agtcaataat caatgtcaac gcgtatatct
     8641 ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt
     8701 gtcggtcaag ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc
     8761 caacacacaa gcagggagca gatactggct taactatgcg gcatcagagc agattgtact
     8821 gagagtgcac cataggggat cgggagatct cccgatccgt cgacgtcagg tggcactttt
     8881 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8941 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9001 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9061 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9121 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9181 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9241 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9301 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9361 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9421 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9481 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9541 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9601 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9661 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9721 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9781 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9841 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9901 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9961 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10021 ggatcttc
//
