LOCUS       sequence-449906-        9119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused 
            BlasticidinR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, 
            Wan J, Fang D, Bintu L
  TITLE     The H3.3 K36M oncohistone disrupts the establishment of epigenetic 
            memory through loss of DNA methylation.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147.
  PUBMED    37873347
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147."
COMMENT     Sequence Label: pLenti-rTetR(SE)-VP64-2A-mCherryBSD
FEATURES             Location/Qualifiers
     source          1..9119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2057..2455)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(2474..3181)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2749..2768)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3018..3036
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(3182..3235)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3245..3394)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(3416..3481)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4122..5060
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4145..4165)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(5061..5272)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    5395..5512
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5810..5854)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6039..6272
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6769..6894
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6941..7121
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7139..7337)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7164..7184)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7339..7718
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7890..7909
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7984..8088
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8089..8157,8158..8949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8307..8326)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc
     2101 caactgccgt cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat
     2161 cgagaagcac ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga
     2221 tcgcgacgat acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga
     2281 gttctgcaca aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt
     2341 cgctagagag agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg
     2401 tagccgttgc tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatgctac
     2461 cgctgccgct acccttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
     2521 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
     2581 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
     2641 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
     2701 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
     2761 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
     2821 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
     2881 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
     2941 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
     3001 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
     3061 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
     3121 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
     3181 ttgggccagg attctcctcg acatctccgc aagttaggag gcttcccctt ccctctccag
     3241 atcccagcat gtccaggtcg aaatcatcaa gggcgtcact gccgagcatg tcgaggtcaa
     3301 agtcatcaag ggcatccgaa ccaagcatgt caaggtcaaa atcatcgagg gcgtcacttc
     3361 ccagcatatc cagatcaaaa tcgtccaatg cgtcggagcc tgatccggat ccacccttgt
     3421 catcgtcatc cttgtagtcg atgtcatgat ctttataatc accgtcatgg tctttgtagt
     3481 caccgccgct ttcgcacttt agctgtttct ccaggccaca tatgattagt tccaggccga
     3541 aaaggaaggc aggttcggct ccctgccggt cgaacagctc aattgcttgt ttcagaagtg
     3601 ggggcataga atcggtggta ggtgtctctc tttcctcttt tgctacttga tgctcctgtt
     3661 cctccaatac gcagcccagt gtaaagtggc ccacggcgga cagagcgtac agtgcgttct
     3721 ccagggagaa gccttgctga cacaggaacg cgagctgatt ttccagggtt tcgtactgtt
     3781 tctctgttgg gcgggtgccg agatgcactt tagccccgtc gcgatgtgag aggagagcac
     3841 agcggtatga cttggcgttg ttccgcagaa agtcttgcca tgactcgggt tccagggggc
     3901 aggagtgggt atgatgcctg tccagcatct cgattggcag ggcatcgagc agggcccgct
     3961 tgttcttcac gtgccagtac agggtaggct gctcaactcc cagcttttga gcgagtttcc
     4021 ttgtcgtcag gccttcgata ccgacaccat tgagtaattc cagagctccg tttattattt
     4081 tgctcttgtc cagtctagac atggtggcta gctcacgaca cctgaaatgg aagaaaaaaa
     4141 ctttgaacca ctgtctgagg cttgagaatg aaccaagatc caaactcaaa aagggcaaat
     4201 tccaaggaga attacatcaa gtgccaagct ggcctaactt cagtctccac ccactcagtg
     4261 tggggaaact ccatcgcata aaacccctcc ccccaaccta aagacgacgt actccaaaag
     4321 cgcgagaact aatcgaggtg cctggacggc gcccggtact ccgtggagtc acatgaagcg
     4381 acggctgagg acggaaaggc ccttttcctt tgtgtgggtg actcacccgc ccgctctccc
     4441 gagcgccgcg tcctccattt tgagctccct gcagcagggc cgggaagcgg ccatctttcc
     4501 gctcacgcaa ctggtgccga ccgggccagc cttgccgccc agggcggggc gatacacggc
     4561 ggcgcgaggc caggcaccag agcaggccgg ccagcttgag actacccccg tccgattctc
     4621 ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg
     4681 tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca gatcttggcc cgcatttaca
     4741 agactatctt gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta aatggctaga
     4801 gacttatcga aagcagcgag acaggcgcga aggtgccacc agattcgcac gcggcggccc
     4861 cagcgcccag gccaggcctc aactcaagca cgaggcgaag gggctcctta agcgcaaggc
     4921 ctcgaactct cccacccact tccaacccga agctcgggat caagaatcac gtactgcagc
     4981 caggtggaag taattcaagg cacgcaaggg ccataacccg taaagaggcc aggcccgcgg
     5041 gaaccacaca cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc
     5101 acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag
     5161 tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc ggttcaattg
     5221 ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg
     5281 gcacatgcat ggcggtaata cggttatcca cgcggccgcc tgagtcaaat gggatctcta
     5341 gagcgggccc ggtactaacc aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt
     5401 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     5461 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     5521 gaatactgcc atttgtctgc agaattggcg cacgcagtgc cgatccgttc actaatcgaa
     5581 tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc
     5641 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
     5701 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
     5761 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
     5821 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
     5881 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
     5941 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
     6001 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
     6061 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
     6121 acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag
     6181 acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg
     6241 ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca
     6301 ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat
     6361 atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca
     6421 gcggccggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc
     6481 taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac
     6541 tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc
     6601 cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc
     6661 tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct
     6721 taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca
     6781 cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg
     6841 cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc
     6901 tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact
     6961 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     7021 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     7081 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct
     7141 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     7201 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     7261 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     7321 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     7381 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     7441 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     7501 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     7561 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     7621 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     7681 aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac
     7741 atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa
     7801 gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca
     7861 agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     7921 ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat
     7981 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8041 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8101 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8161 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8221 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8281 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8341 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8401 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8461 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8521 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8581 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8641 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8701 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8761 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8821 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8881 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8941 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9001 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9061 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
