LOCUS       sequence-449908-       10101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry 
            fused BlasticidinR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, 
            Wan J, Fang D, Bintu L
  TITLE     The H3.3 K36M oncohistone disrupts the establishment of epigenetic 
            memory through loss of DNA methylation.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147.
  PUBMED    37873347
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147."
COMMENT     Sequence Label: pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD
FEATURES             Location/Qualifiers
     source          1..10101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2057..2455)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(2474..3181)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2749..2768)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3018..3036
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(3182..3235)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3245..3271)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3284..3310)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3311..3514)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3521..3724)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3731..3934)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3959..3985)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3986..4189)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4196..4399)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4406..4609)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4634..4660)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          5104..6042
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5127..5147)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(6043..6254)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    6377..6494
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6792..6836)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7021..7254
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7751..7876
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7923..8103
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8121..8319)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8146..8166)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8321..8700
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8872..8891
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8966..9070
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9071..9139,9140..9931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9289..9308)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc
     2101 caactgccgt cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat
     2161 cgagaagcac ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga
     2221 tcgcgacgat acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga
     2281 gttctgcaca aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt
     2341 cgctagagag agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg
     2401 tagccgttgc tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatgctac
     2461 cgctgccgct acccttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
     2521 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
     2581 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
     2641 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
     2701 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
     2761 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
     2821 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
     2881 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
     2941 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
     3001 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
     3061 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
     3121 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
     3181 ttgggccagg attctcctcg acatctccgc aagttaggag gcttcccctt ccctctccag
     3241 atccagcata atcaggcaca tcataagggt atccggcgct gccagcgtag tcaggcacat
     3301 cataggggta tttatacatt cgcagatcaa ttttccgcaa gtagggggct ccgtccatac
     3361 ttacttttac aaatgctgcc gcctcggggc ctccagaact cttttggcaa ctgaccatta
     3421 cattcttgcg gtatgatctg acggggggcc aaccgactac ttgagctttt gccgggggtt
     3481 tagctgggtc tttcggacat gcactcttct ctttggaaga tttatacatg cggagatcta
     3541 ttttcctaag gtacggggcg ccgtccatgc taactttaac gaatgctgca gcctctggtc
     3601 cgccactaga cttttgacaa gataccataa cattcttgcg ataggatctg acaggcggcc
     3661 aaccaactac ttgagctttg gcgggtggct tcgcagggtc cttcgggcag gcgctctttt
     3721 ctttgctgct cttatacatc ctaaggtcga ttttcctcaa gtacggcgca ccatccatgg
     3781 aaacctttac aaacgctgcg gcctctggac ctccgcttga tttttgacat gataccataa
     3841 catttttcct gtatgagcgc acaggaggcc aaccgacgac ctgagccttc gccggcggct
     3901 ttgcagggtc ttttggacag gcacttttct ccttactcaa ttggccggaa atcgatggtg
     3961 cataatcagg cacatcatag gggtatttat acattcgcag atcaattttc cgcaagtagg
     4021 gggctccgtc catacttact tttacaaatg ctgccgcctc ggggcctcca gaactctttt
     4081 ggcaactgac cattacattc ttgcggtatg atctgacggg gggccaaccg actacttgag
     4141 cttttgccgg gggtttagct gggtctttcg gacatgcact cttctctttg gaagatttat
     4201 acatgcggag atctattttc ctaaggtacg gggcgccgtc catgctaact ttaacgaatg
     4261 ctgcagcctc tggtccgcca ctagactttt gacaagatac cataacattc ttgcgatagg
     4321 atctgacagg cggccaacca actacttgag ctttggcggg tggcttcgca gggtccttcg
     4381 ggcaggcgct cttttctttg ctgctcttat acatcctaag gtcgattttc ctcaagtacg
     4441 gcgcaccatc catggaaacc tttacaaacg ctgcggcctc tggacctccg cttgattttt
     4501 gacatgatac cataacattt ttcctgtatg agcgcacagg aggccaaccg acgacctgag
     4561 ccttcgccgg cggctttgca gggtcttttg gacaggcact tttctcctta ctcaattggc
     4621 cggaaatcga tggtgcataa tcaggaacat cataaggata gccaccgtta acagctctct
     4681 ctccccgtat ccggcgagcc aactggatgt ctttgggcat gatggtgact ctcttagcgt
     4741 ggatggcaca caggttggta tcttcgaaca gacccaccag gtacgcttcg ctagcctcct
     4801 gcagcgcacc gatggctgcg ctctgaaacc tcaggtcggt tttgaaatcc tgcgcgatct
     4861 ccctcaccaa cctctggaag ggcagcttcc ggatgagcag ctcggtcgac ttctgataac
     4921 gacgaatctc tcgaagcgcc acggtcccgg gcctgtagcg atgaggcttc ttcaccccgc
     4981 cggtagaggg agcgctcatc ctggcggctt tcgtggccag ctgtttgcgg ggggctttcc
     5041 caccggtgga cttacgagca gtctgtttgg ttcgggccat atttaaatgc tagctcacga
     5101 cacctgaaat ggaagaaaaa aactttgaac cactgtctga ggcttgagaa tgaaccaaga
     5161 tccaaactca aaaagggcaa attccaagga gaattacatc aagtgccaag ctggcctaac
     5221 ttcagtctcc acccactcag tgtggggaaa ctccatcgca taaaacccct ccccccaacc
     5281 taaagacgac gtactccaaa agcgcgagaa ctaatcgagg tgcctggacg gcgcccggta
     5341 ctccgtggag tcacatgaag cgacggctga ggacggaaag gcccttttcc tttgtgtggg
     5401 tgactcaccc gcccgctctc ccgagcgccg cgtcctccat tttgagctcc ctgcagcagg
     5461 gccgggaagc ggccatcttt ccgctcacgc aactggtgcc gaccgggcca gccttgccgc
     5521 ccagggcggg gcgatacacg gcggcgcgag gccaggcacc agagcaggcc ggccagcttg
     5581 agactacccc cgtccgattc tcggtggccg cgctcgcagg ccccgcctcg ccgaacatgt
     5641 gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca aaaaccgaaa taccagtgtg
     5701 cagatcttgg cccgcattta caagactatc ttgccagaaa aaaagcgtcg cagcaggtca
     5761 tcaaaaattt taaatggcta gagacttatc gaaagcagcg agacaggcgc gaaggtgcca
     5821 ccagattcgc acgcggcggc cccagcgccc aggccaggcc tcaactcaag cacgaggcga
     5881 aggggctcct taagcgcaag gcctcgaact ctcccaccca cttccaaccc gaagctcggg
     5941 atcaagaatc acgtactgca gccaggtgga agtaattcaa ggcacgcaag ggccataacc
     6001 cgtaaagagg ccaggcccgc gggaaccaca cacggcactt acctgtgttc tggcggcaaa
     6061 cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc
     6121 ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct
     6181 tctctaggca ccggttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga
     6241 tgtgcgctct gcccactgac gggcacatgc atggcggtaa tacggttatc cacgcggccg
     6301 cctgagtcaa atgggatctc tagagcgggc ccggtactaa ccaaactgga tctctgctgt
     6361 ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt
     6421 gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc
     6481 cttttctttt aaaattgtgg atgaatactg ccatttgtct gcagaattgg cgcacgcagt
     6541 gccgatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct
     6601 attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
     6661 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
     6721 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta
     6781 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
     6841 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct
     6901 ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa
     6961 ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc
     7021 aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg
     7081 atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg
     7141 ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac
     7201 gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc
     7261 actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat
     7321 ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct
     7381 cctcctccag gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt
     7441 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
     7501 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
     7561 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
     7621 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
     7681 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
     7741 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
     7801 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
     7861 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
     7921 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     7981 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     8041 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     8101 cccagtacag gcaaaacgcg ctgcttatat agacctccca ccgtacacgc ctaccgccca
     8161 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     8221 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     8281 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     8341 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     8401 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     8461 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     8521 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     8581 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     8641 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
     8701 aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat
     8761 tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact
     8821 actcagcgac ctccaacaca caagcaggga gcagatactg gcttaactat gcggcatcag
     8881 agcagattgt actgagagtg caccataggg gatcgggaga tctcccgatc cgtcgacgtc
     8941 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9001 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9061 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9121 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9181 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9241 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9301 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9361 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9421 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9481 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9541 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9601 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9661 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9721 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9781 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9841 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9901 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9961 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10021 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10081 agaaaagatc aaaggatctt c
//
