LOCUS       sequence-44993-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt1
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..51)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    77..101
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg
       61 cgggccctct agatcaacca ctttgtacaa gaaagctggg tcggcgcgcc cacccttcag
      121 aattcacaga cactcgtgca gtacgcgcgt gtgcgtgcag ttgcggcagc tgacgtggca
      181 gcaccagtgg aaggtgcagt tgcagcgctc ggtgacgcgc tgcgtgcgcg tgcggtggcc
      241 cctgccgcag cagagcagct cgcagccgtc cagcgcgggc gacgagctgt tacaggcgcg
      301 ccctgccgtg cctgctgtgc ccaggcgtcc gctgtacgtg cagaagttgg gcgatttctc
      361 gaagtagacg aggtcgtggg gggagggcgg tttgtgggcc gggtcttccg gctccaggcg
      421 cagcagctcc gcccgcgaag cgcggttgct gccgcggttg ccgtacagga cgcgcgaggc
      481 gccgtcgaag cggtcgcgca gcacatcgcc cacggcgcgc agcgtgggca gccgcatcca
      541 gcacgtgcgc accgtgcatg agccggacat cccgtggcac ttgcactcct ggcgcatctc
      601 ggagaatacg gtcgtacggc ctgcctcgtt gttgtgaagg ttcatgagga agcgcaggtc
      661 ccgccccttc tccccggagt ccacgaactc ccggccgaag aggcggccga agtcaatgtt
      721 gtcgctgcag cccccccagt gccagtcggg gcccccgggg ccgcgccgcc ggtagtcaca
      781 cgtgcaggat tcgatggaac cttctgagca ggagcgcgcc accgaatggg tgaccccggc
      841 ggaggtgata gcgaagataa acgccgtt
//
