LOCUS       sequence-44994-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Srinivas H, Juroske DM, Kalyankrishna S, Cody DD, Price RE, Xu XC, 
            Narayanan R, Weigel NL, Kurie JM
  TITLE     c-Jun N-terminal kinase contributes to aberrant retinoid signaling 
            in lung cancer cells by phosphorylating and inducing proteasomal 
            degradation of retinoic acid receptor alpha.
  JOURNAL   Mol Cell Biol. 2005 Feb;25(3):1054-69.
  PUBMED    15657432
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-02"; volume: "25(3)"; pages: "1054-69"
COMMENT     Sequence Label: pcDNA Flag-RARalpha (T181A,S445A,S461A)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             114..131
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             135..167
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(171..179,180..194)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             207..272
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gcgtttaaac ttaagcttac catggggggt tctcatcatc
      121 atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg
      181 acgatgacga taaggtacca ggatccgact acaaagacca tgacggtgat tataaagatc
      241 atgacatcga ttacaaggat gacgatgaca agcttgcggc cgcgaattca gccagcaaca
      301 gcagctcctg cccgacacct gggggcgggc acctcaatgg gtacccggtg cctccctacg
      361 ccttcttctt cccccctatg ctgggtggac tctccccgcc aggcgctctg accactctcc
      421 agcaccagct tccagttagt ggatatagca caccatcccc agccaccatt gagacccaga
      481 gcagcagttc tgaagagata gtgcccagcc ctccctcgcc accccctcta ccccgcatct
      541 acaagccttg ctttgtctgt caggacaagt cctcaggcta ccactatggg gtcagcgcct
      601 gtgagggctg caagggcttc ttccgccgca gcatccagaa gaacatggtg tacacgtgtc
      661 accgggacaa gaactgcatc atcaacaagg tgacccggaa ccgctgccag tactgccgac
      721 tgcagaagtg ctttgaagtg ggcatgtcca aggagtctgt gagaaacgac cgaaacaaga
      781 agaagaagga ggtgcccaag cccgagtgct ctgagagcta cacgctggcg ccgga
//
