LOCUS       sequence-449963-        8865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Nuclease active S. gallolyticus Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Butterfield GL, Rohm D, Roberts A, Nethery MA, Rizzo AJ, Morone DJ, 
            Garnier L, Iglesias N, Barrangou R, Gersbach CA
  TITLE     Characterization of diverse Cas9 orthologs for genome and epigenome 
            editing.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Mar 18;122(11):e2417674122. doi: 
            10.1073/pnas.2417674122. Epub 2025 Mar 12.
  PUBMED    40073054
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-03-18"; volume: "122(11):e2417674122. 
            doi"; pages: " 10.1073/pnas.2417674122. Epub 2025 Mar 12"
COMMENT     Sequence Label: pcDNA-Sga-Cas9
FEATURES             Location/Qualifiers
     source          1..8865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..972
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             982..1002
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4414..4434
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4456..4473)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4462..4686
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4732..5160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4819..4838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5029..5050
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5169..5189)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5174..5503
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5354..5489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5416..5435
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5570..6364
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5624..5643)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6234..6253
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6538..6659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6575..6594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6629..6648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6708..6724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6708..6724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6721..6743)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6732..6748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6756..6762,6763..6780,6781..6786))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6801..6822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6939..6956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7110..7698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7190..7209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7869..8660,8661..8729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8492..8511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8730..8834)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
      961 gacgatgaca agcacgttaa ccccaagaag aagaggaagg tgggccgcgg aaccggtatg
     1021 acaaacggca aaattctggg tctggatatc ggaatcgcta gcgttggcgt gggaatcatt
     1081 gaagcgaaga caggtaaagt cgtccatgca aattctcgat tgttctccgc agctaacgct
     1141 gaaaacaatg cggagagaag gggtttcaga ggctctaggc ggctcaaccg gcgcaagaag
     1201 cacagggtaa aaagagtgcg agatctcttt gagaaatatg agatcgtgac tgattttaga
     1261 aacctgaatc tgaacccata tgagctgaga gtgaaaggac ttacggaaca gctcactaat
     1321 gaagagttgt tcgccgccct gcggaccatc agcaaacgcc gaggaatttc ctaccttgat
     1381 gacgccgaag atgacagtac cggtagcaca gattatgcca agagcattga tgagaacagg
     1441 agactgctga agactaagac acctggacag atacaattgg aacggctcga gaagtacggc
     1501 cagctgaggg gtaacttcac cgtttatgac gaaaatgggg aggcccatag actgataaat
     1561 gtgttctcaa cttctgacta tgaaaaggag gcccggaaaa tcctcgagac tcaagccgac
     1621 tacaacaaga agattacagc cgagtttatt gacgattacg tggaaatttt aacccagaaa
     1681 aggaagtatt accacgggcc aggaaatgaa aagagccgca ccgactatgg gagattcaga
     1741 acggatggaa caaccttaga gaatatcttt ggaatcctta ttggtaaatg ctctttctat
     1801 cctgacgagt atcgcgccag caaagcctcc tataccgctc aggagtacaa cttcttgaat
     1861 gatttgaaca atttgaaggt tccgacggag actggcaagc tgagtaccga gcaaaaggag
     1921 gcccttgtgg aattcgccaa gtctactgca acattaggtc ctgctaaact tctgaaggag
     1981 attgccaaaa ttttggactg caaagtcgat gaaatcaagg ggtaccgtga ggatgataaa
     2041 gggaaaccag acctgcacac ctttgagccc tatagaaagt tgaaattcaa tctggacagc
     2101 gtcaacattg acgatttgag tcgcgaagtg ctggacaagc tggcagacat tttgacactt
     2161 aacactgaaa gggagggcat tgaggatgcc atcaggcata acctgcccaa ccaatttact
     2221 gagggccaga tctccgaaat catcaaggtg cgcaaaagcc agagcactgc tttcaacaag
     2281 gggtggcaca gcttctctgc caagctcatg aacgaattga ttcccgagct ctatgccaca
     2341 agcgacgaac agatgactat acttactcgg ctggagaaat ttaaggtcaa taaaaaatcc
     2401 tccaaaaaca ccaagacgat tgacgagaaa gaggtcactg atgaaatcta caatccagtt
     2461 gtagccaagt ctgtccggca aacgatcaag atcattaacg ctgctgtgaa gaaatatgga
     2521 gactttgata agattgtgat tgaaatgcct cgcgacaaga atgcggacga tgagaagaag
     2581 tttatcgata agagaaacaa agaaaataag aaagaaaagg atgatgccct gaagcgggca
     2641 gcttaccttt ataatggaac cgataagctg ccagatgagg tgtttcacgg aaacaagcaa
     2701 cttgaaacca agattcgcct gtggtaccag cagggagaac ggtgtttgta ctcaggcaag
     2761 cctatcccaa tccaggagtt ggtccacaac tccaataact tcgaaatcga tcacattctg
     2821 cccctgtccc tgagttttga cgactccctg gccaacaagg tgcttgtgta tgcttggacc
     2881 aaccaagaga agggccagaa gacgccctac caggtgattg attctatgga tgcggcgtgg
     2941 tcctttcgcg agatgaagga ctatgtgctc aagcaaaaag gcctcggcaa aaagaaacgg
     3001 gattatcttt tgaccaccga gaacattgac aagattgaag tgaagaaaaa attcatcgag
     3061 cgcaacttgg tcgataccag atatgcctct agggttgtgc tgaactcact gcagtctgct
     3121 ttgagagagc tgggtaaaga cactaaaatt agtgtaatca ggggccagtt cacaagtcag
     3181 cttaggcgga aatggaagat cgacaagtca cgcgagacat atcatcatca cgcagtcgac
     3241 gcactgataa ttgcagcttc aagtcagctc aagttgtggg agaaacagga taaccctatg
     3301 tttgtcgact atggaaacaa tcaggtcgtc gataagcaga ccggggaaat tttaagtgtg
     3361 tccgatgacg agtataagga gcttgtcttt cagccaccgt accagggctt tgtcaacatg
     3421 attagtagca agggttttga ggacgaaatt ttgttcagct accaggtcga ttccaaatac
     3481 aatagaaaag tatccgacgc aaccatatat tctactcgca aggccaagat tggcaaagat
     3541 aagaaggaag agacctatgt attggggaag atcaaagaca tttactcaca aaatggattc
     3601 gacaccttca ttaagaagta caacaaagat aagacacagt ttttgatgta ccagaaagat
     3661 ccactgacat gggaaaacgt gatcgaagtt atactgcgtg actaccccac gactaaaaag
     3721 agtgaggacg gaaaaaacga cgtgaagtgc aacccgtttg aagaataccg gagagaaaac
     3781 ggtctggtgt gtaagtactc taagaaagga aaggggaccc ctattaaatc cctcaaatac
     3841 tacgacaaaa aactcgggaa ctgcatcgat atcaccccgg aaggttccaa aaatgaagtc
     3901 gtgcttcaat ccttgaatcc gtggagggca gatgtgtact ttaacccaga aaccttgaag
     3961 tatgaattac tgggacttaa atacagtgat ctctcatttg aaaagggcac tggaaaatac
     4021 catatctctc aggagaagta cgacgtcatt aaggaaaaag aagggatcgg gaaaaaatcc
     4081 gagttcaagt tcacattgta taggaacgac ctgatcctta ttaaagacac agccagcggt
     4141 gagcaggaga tttaccgatt tctgtctaga accatgccta acgtcaagca ctatgcggag
     4201 ctgaagccct atgacaaaga aaaatttgat aacgtccagg aactcgtcga ggcgctgggc
     4261 gaagccgaca aggtaggccg ctgtataaag gggctgaaca aaagcaacct cagcatctat
     4321 aaagttagga cagatgtgct cgggaacaaa tacttcgtta agaaggaagg ggacaagccc
     4381 aagctggatt ttaagaacaa taaaaagacc ggtcctaaga aaaagcggaa agtggctgtt
     4441 taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4501 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4561 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4621 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4681 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     4741 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4801 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4861 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4921 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4981 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5041 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5101 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5161 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5221 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5281 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5341 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     5461 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     5521 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     5581 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5641 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5701 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5761 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5821 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5881 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5941 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6001 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6061 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     6121 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6181 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6241 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6301 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     6361 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     6421 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     6481 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     6541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6661 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6721 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6781 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6841 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6901 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7801 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7861 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7921 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7981 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8041 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8101 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8161 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8221 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8281 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8341 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8401 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8461 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8521 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8581 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8641 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8701 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8761 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8821 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
