LOCUS       sequence-449969-        9579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Nuclease active S. iniae Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Butterfield GL, Rohm D, Roberts A, Nethery MA, Rizzo AJ, Morone DJ, 
            Garnier L, Iglesias N, Barrangou R, Gersbach CA
  TITLE     Characterization of diverse Cas9 orthologs for genome and epigenome 
            editing.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Mar 18;122(11):e2417674122. doi: 
            10.1073/pnas.2417674122. Epub 2025 Mar 12.
  PUBMED    40073054
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-03-18"; volume: "122(11):e2417674122. 
            doi"; pages: " 10.1073/pnas.2417674122. Epub 2025 Mar 12"
COMMENT     Sequence Label: pcDNA-Sin-Cas9
FEATURES             Location/Qualifiers
     source          1..9579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..972
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             982..1002
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5128..5148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5170..5187)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5176..5400
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5446..5874
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5533..5552)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5743..5764
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5883..5903)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5888..6217
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6068..6203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6130..6149
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6284..7078
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6338..6357)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6948..6967
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7252..7373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7289..7308)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7343..7362
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7422..7438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7422..7438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7435..7457)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7446..7462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7470..7476,7477..7494,7495..7500))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7515..7536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7653..7670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7824..8412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7904..7923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8583..9374,9375..9443))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9206..9225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9444..9548)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
      961 gacgatgaca agcacgttaa ccccaagaag aagaggaagg tgggccgcgg aaccggtatg
     1021 cgcaaacctt actcaattgg cctggatatc gggactaatt ctgttggctg ggctgtgatt
     1081 actgatgatt acaaggtgcc aagtaagaaa atgaggattc agggcacgac tgatcggacc
     1141 agcattaaga agaatctcat tggggccctc ctgttcgata atggcgagac tgccgaggcc
     1201 actcgattaa agagaacaac aaggaggagg tacaccagac ggaagtaccg aataaaggaa
     1261 ctgcaaaaga tcttcagcag cgaaatgaat gagctcgaca ttgctttttt ccctagactg
     1321 tctgagagtt ttcttgtgag tgacgacaaa gaattcgaga atcatccgat ttttggaaac
     1381 cttaaagatg agataactta tcataacgat taccctacta tttatcactt gcgacagaca
     1441 cttgcagacc gtgaccagaa ggccgatctt aggctcattt atctcgctct ggcccacatt
     1501 attaaatttc gggggcactt tttgatcgaa ggcaatctgg acagtgagaa cacggacgta
     1561 cacgtgctgt ttctgaacct ggtgaacata tataataacc tgttcgagga agatatagtt
     1621 gaaaccgcat ccatagacgc tgagaagatt cttacctcaa aaacttccaa atccaggcgg
     1681 ctcgagaatc ttatagctga gattcctaac cagaagcgga acatgttgtt tggcaacctc
     1741 gtgtctctgg ctctcggcct gacaccaaat tttaaaacca attttgagct gctggaggat
     1801 gcaaagttac agatctccaa ggattcatat gaagaagacc tcgacaactt gttggcacag
     1861 attggggatc agtacgcaga tctctttatc gccgctaaaa agctttctga cgcaatatta
     1921 ctgtctgaca tcatcaccgt gaagggcgcc tccactaaag cgcctctttc agcatccatg
     1981 gtgcagagat atgaagagca tcaacaggac ctcgctctcc tgaagaatct cgtgaaaaaa
     2041 cagattcctg agaagtataa ggaaatcttc gataacaagg agaagaatgg ctatgcaggt
     2101 tatatcgatg gcaagacctc ccaggaggaa ttttacaagt acatcaagcc catacttctt
     2161 aagctcaacg gcacagagaa gttgatcagc aaacttgagc gggaggactt cctgagaaag
     2221 caacgaacat tcgacaacgg atctattcct caccagattc acctgaatga gctcaaggca
     2281 atcatccgga ggcaggagaa gttttatccc tttctgaagg aaaatcagaa gaaaatcgaa
     2341 aagcttttca catttaaaat tccctattac gtcgggccac tcgccaatgg ccagagtagc
     2401 ttcgcctggc tgaagagaca gtccaacgag tctatcaccc cctggaactt cgaggaagtg
     2461 gtggatcaag aggcctcagc gcgcgccttc atagagagga tgactaactt cgatacctat
     2521 ttacccgagg agaaggttct gccaaagcac agcccactct acgaaatgtt tatggtctat
     2581 aatgagctca ccaaggttaa gtatcagacc gaggggatga agaggcccgt ctttctctct
     2641 tccgaagaca aagaagaaat agtgaatctc ctgttcaaaa aagaccggaa ggtcactgtc
     2701 aagcagctga aggaggaata tttctccaaa atgaaatgct tccacaccgt gacaatcttg
     2761 ggcgtggagg atcggtttaa tgcttctctg ggcacgtacc atgacctgct caaaattttt
     2821 aaagataaag ccttcttaga cgatgaggcc aatcaagata tcttggaaga gatcgtatgg
     2881 actttaacgc tttttgagga tcaagccatg attgaaagaa ggctggtgaa gtacgcggac
     2941 gtgttcgaaa aatccgtcct taaaaagtta aagaaacgcc attacacggg ctggggacgt
     3001 ctttcccaga agcttattaa tgggatcaaa gacaaacaaa ctgggaagac aattctcggc
     3061 tttctgaaag acgacggtgt agccaaccga aattttatgc agttaattaa cgacagctcc
     3121 ctggacttcg caaagattat caagcatgaa caggaaaaaa ccatcaagaa cgagtcattg
     3181 gaggaaacga ttgcgaacct ggcaggcagc cccgccatta agaaaggcat tcttcagtct
     3241 attaaaattg tcgatgaaat cgttaagatt atgggacaga acccagacaa tattgttatt
     3301 gagatggcac gcgagaacca atccacgatg caaggaatca aaaactcccg acagcgtctg
     3361 cgcaagctcg aggaggtgca taagaacacc gggtccaaga ttttgaaaga atacaacgtg
     3421 agtaatacgc agcttcagag cgataggctc tatttatacc tgctgcagga cggaaaggat
     3481 atgtacaccg gcaaggagtt ggactacgac aatcttagtc aatatgatat tgatcacatc
     3541 atccctcagt ctttcataaa agataactct atcgacaaca tagtgctgac tacacaagct
     3601 agtaataggg gcaagtcaga caacgtgccc aacatagaga ttgtgaacaa aatgaagtct
     3661 ttttggtata aacagctcaa aaatggggca attagccagc gcaaattcga ccatttaacc
     3721 aaggccgagc gtggcgcact gagcgatttc gataaggcag gctttatcaa gcgccagctc
     3781 gtcgagacac ggcagataac caaacatgtg gctcaaatcc tggacagtcg gttcaattcc
     3841 aatcttacgg aggactctaa atctaacaga aacgttaaga taataactct caagtcaaaa
     3901 atggtgagtg acttccgaaa ggactttggc ttttacaagc tgagagaagt aaatgattat
     3961 caccacgccc aggacgcata tctcaatgcc gtcgtcggta ctgccttact taagaagtac
     4021 cctaaactgg aagcagagtt cgtgtatggg gattacaagc actacgatct cgctaagtta
     4081 atgattcaac cggacagtag ccttggaaaa gccacaacca gaatgttctt ctattctaac
     4141 ctcatgaatt tcttcaaaaa agaaatcaaa ctggccgatg atactatatt tacgaggccc
     4201 cagattgaag tgaacaccga aactggggag attgtctggg ataaggtaaa ggacatgcag
     4261 accatcagga aagtgatgtc ctatccacaa gtcaacatag tgatgaaaac cgaagtccag
     4321 actggggggt tttctaagga gagtatcctg cctaagggaa actcagacaa actgatcgcc
     4381 cgcaagaaat cctgggaccc taagaaatac ggtggtttcg atagccctat cattgcatat
     4441 tcagtcctgg tcgtcgctaa gatagccaaa ggcaaaaccc agaaactcaa gactattaaa
     4501 gagttggtcg gtatcaaaat catggagcag gacgaattcg aaaaggatcc aattgcgttt
     4561 ctcgaaaaga agggctatca ggacatacag acctcttcca tcatcaagct gccgaagtac
     4621 tctctctttg agcttgagaa tggacgcaag agactgctgg ctagcgccaa agaactgcag
     4681 aagggcaacg aactggccct ccctaacaaa tacgtaaagt tcttgtattt agcatctcat
     4741 tacacaaaat tcacaggtaa ggaggaagat cgagaaaaaa agcgctccta tgtagagtca
     4801 cacctgtatt actttgacga gattatgcag attatcgttg agtattctaa ccggtacatt
     4861 ctcgccgaca gcaatctgat taaaattcag aacttgtaca aagagaagga taactttagt
     4921 atcgaggagc aagccattaa tatgctcaat ctcttcactt ttacagatct cggcgcgcca
     4981 gccgctttca agttctttaa cggagatata gatcggaagc ggtacagctc taccaacgag
     5041 atcattaatt ctactctgat ttaccagagt cccacagggt tatacgagac caggatcgac
     5101 ctcagtaagc tggggggcaa aaccggtcct aagaaaaagc ggaaagtggc tgtttaaacc
     5161 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5221 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5281 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5341 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     5401 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     5461 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5521 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5581 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5641 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5701 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5761 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5821 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     5881 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5941 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6001 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6061 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6121 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6181 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6241 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     6301 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6361 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     6421 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     6481 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6541 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6601 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6661 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6721 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6781 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     6841 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6901 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6961 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7021 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     7081 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     7141 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     7201 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     7261 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7321 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7381 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7441 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7501 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7561 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7621 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7681 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7741 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8221 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8461 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8521 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8581 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8641 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8701 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8761 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8821 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8881 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8941 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9001 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9061 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9121 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9181 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9241 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9301 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9361 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9421 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9481 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9541 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
