LOCUS       sequence-449977-        9074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing human LMNB1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9074)
  TITLE     Lentiviral plasmids expressing popular genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti puro HA-LMNB1
FEATURES             Location/Qualifiers
     source          1..9074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    626..751
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             798..978
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(979..1205)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1233..1253)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1233..1251)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1302..1318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1302..1318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1315..1337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1326..1342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1350..1356,1357..1374,1375..1380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1395..1416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1533..1550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1704..2292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1784..1803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2463..3254,3255..3323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3086..3105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3324..3428)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3496..3514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3552..3574)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3674..3693
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3887..3909
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3901..3918
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3902..3918
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3950..3969
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3950..3968
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3989..4124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4164..4285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4175..4194)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4229..4248
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4357..4590
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4718..5317)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4801..4821)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5298..5317
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5339..5849)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5402..5420)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    5898..6015
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7861..7887)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(7935..8138)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7964..7984)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(8427..8447)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(8745..8789)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(8974..9074,1..133)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
       61 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
      121 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
      181 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
      241 ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct tgctgtgcgg
      301 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
      361 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
      421 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg
      481 atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta
      541 tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg
      601 atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc
      661 cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg
      721 cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt
      781 ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct
      841 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
      901 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
      961 gtctaaccag agagacccgt ttatgtatcg agctaggcac ttaaatacaa tatctctgca
     1021 atgcggcaat tcagtggttc gtccaatcca tgtcagaccc gtctgttgcc ttcctaataa
     1081 ggcacgatcg taccacctta cttccaccaa tcggcatgca cggtgctttt tctctccttg
     1141 taaggcatgt tgctaactca tcgttaccat gttgcaagac tacaagagta ttgcataaga
     1201 ctacattaag cttgcagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     1261 gcgtaatcat gctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1321 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1381 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1441 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1501 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1861 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2101 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2341 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2401 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2461 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2521 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2581 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2761 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2821 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2881 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2941 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3001 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3061 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3121 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3181 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3241 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3301 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3421 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3481 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     3541 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     3601 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     3661 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     3721 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     3781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     3841 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     3901 tgtaaaacga cggccagtga attcgagctc ggtacccggg gagcgcgcgt aatacgactc
     3961 actatagggc gaattgggta cgtccctagg cctccaaaaa agcctcctca ctacttctgg
     4021 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     4081 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     4141 gggatagcta gagccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     4201 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     4261 cattataagc tgcaataaac aagttcctct cactctctga tattcatttc tttgcaagtt
     4321 ataaatactg aataataaga tgacatgaac tactactgct agagattttc cacactgact
     4381 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     4441 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     4501 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt
     4561 gtcttcgttg ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa
     4621 gatctacagc tgccttgtaa gtcattggtc ttaaaggtac cgatgcatgg ggtcgtgcgc
     4681 tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca
     4741 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
     4801 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
     4861 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
     4921 cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
     4981 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
     5041 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
     5101 ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
     5161 agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg
     5221 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc
     5281 gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt aagctccggt ggatccccct
     5341 ggggagagag gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag
     5401 gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc
     5461 ccgcctcccg caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc
     5521 tattggccac agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg
     5581 cgagggtact agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca
     5641 aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag
     5701 cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg
     5761 cgccgctgcg tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc
     5821 tgccttggaa aaggcgcaac cccaaccccc tcgagccgcg gccaaagtgg atctctgctg
     5881 tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt
     5941 tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc
     6001 ccttttcttt taaaattgtg gatgaatact gccatttgtc tcgaggtcga gaattctcta
     6061 gagaagcttg agctcgagat ctgagtccgg ctacatgatg gcacaggatc gattgctggc
     6121 gcgaggagta ccctgttggt gaaagagttc ctcttcaacg acgacaccgg ctgcctcttc
     6181 ctcctcttct tcctcctctg gtattgtcgt cttaaacacg gtacttcgct gagcgacctc
     6241 ctctccttgt gaattcttca gtatcacctt cacgtcctct ccggtccccc atgagttctg
     6301 gttcttccag atcaaatcag ttgggggaga ggctgttacc ccagcatttg cagcccagat
     6361 agtaactgtc tgaccggcct taagaacgta ccgggaagtg tatttatagc tgacgctcgt
     6421 gtccccaatc ttccggatca tttcccaacc acccatgggt tgatcttgct cacttgtatt
     6481 tttaagcctt ataaattttc cgtcgacatc aatttcctcg atgcagacat ttccggtagc
     6541 tgaggctgaa tgactgattg acacgctgga ggaagcttcg gactcctcta cgtcaacgcg
     6601 ctttcgttta ccgcgcgtgg tgcgtactga cctggaggag gacgctcgag agacggtgac
     6661 ccttgaactg ggagacgggg agagcttcag tctttcttct tccccttcaa gcagcttcct
     6721 atacgctgag atctccatgt ccagtgcgag cttcacgtcg aggagttgtt catagtcatt
     6781 aagttgttgt tgcatttggt cgcgaatttc agccatctcc cgttccttat ctgtcaacat
     6841 gcgcctgcta ttatcttttt ctttggccag gaggtcctcg agttcctgaa tcctttccag
     6901 acaggcccga ctctccttct gaaggttaga cagttgactg gacagtgact cgattcgcat
     6961 ccgtgattcc atgagctctt ccctagctga atttactgtt gacgtattca tctcgctaga
     7021 gagtctagca ttttcaagtt tggcatggta ggtctgctca agttcctctt tgtacagcct
     7081 aacctgggcg tcgtgctgtt cgcgcatctc atggagtgcc tgagccagct tatactcata
     7141 ttcaatttgc cggccggaat ctacctccac aaggcgagtt tcatgtttcc tccgtgtctc
     7201 gttaatttct tcttcgtaca tgctttttcg aaattccaga tcctctgtca gggattgaca
     7261 cctgttctca agatcgactt tgagcagggt ctcatcagcg agttgttttt tggccgcggc
     7321 caggctggcc tcaagttggg cgatctgatc tttgaggtct tccaagtctc cctccaagga
     7381 ctttttgtca ccgagtgctg tggccagggc ggcgtctttg ctattaaggg ctgcctcgta
     7441 ttctcgaagc ttgatctggg caccattcaa gtcactctct ttcttcgcgt agttaaggag
     7501 aagttggtcg tgctcggctt tgcatttgcc caactcaatt tgcagtttag ccctctctct
     7561 cgctgtatcg tccagggccc tgcgtgcatc ggccaattca gtctcataca aagctttcag
     7621 cccggtgagt tcgcggcctc taacctcttc ccgctccgtt acctggagct gcaaagctga
     7681 gttctctgtc tccaaggagc gaaccttgtc aatatagacc gccagtcgat cgttaagttc
     7741 tcgcaactct tccttttctt gcaacctact cagtcttgtc gggctcaacg gtgtcgtagg
     7801 accgccggcc cgtgacccca ttcgaggagg cacaggcgtc gcagtagcca tggatccgct
     7861 agcgtaatcc ggaacatcgt atgggtacat ggtggcgacc ggtagcgcta gcggatctga
     7921 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     7981 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     8041 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     8101 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     8161 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     8221 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atagtccatt
     8281 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
     8341 ttttgtacta atatctttgt gtttacagtc aaattaattc caattatctc tctaacagcc
     8401 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcggt gaagggggcg
     8461 gccgctcgag gctagtctcg tgatcgatac cgtcgagatc cgttcactaa tcgaatggat
     8521 ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc
     8581 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
     8641 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
     8701 aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta
     8761 tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag
     8821 gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga
     8881 ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg
     8941 caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt
     9001 tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga
     9061 tgctgttgcg cctc
//
