LOCUS       sequence-449980-        8669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing human TUBA1B protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8669)
  TITLE     Lentiviral plasmids expressing popular genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti puro HA-TUBA1B
FEATURES             Location/Qualifiers
     source          1..8669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    424..549
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             596..776
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(777..1003)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1031..1051)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1031..1049)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1100..1116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1100..1116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1113..1135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1124..1140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1148..1154,1155..1172,1173..1178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1193..1214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1331..1348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1502..2090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1582..1601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2261..3052,3053..3121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2884..2903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3122..3226)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3294..3312
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3350..3372)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3472..3491
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3685..3707
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3699..3716
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3700..3716
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3748..3767
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3748..3766
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3787..3922
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3962..4083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3973..3992)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4027..4046
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4155..4388
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4516..5115)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4599..4619)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5096..5115
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5137..5647)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5200..5218)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    5696..5813
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7254..7280)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(7328..7531)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7357..7377)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(7820..7840)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(8138..8182)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8367..8600
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tcaattttta ctactttata tttatataat tcacttctcc aattgtccct catatctcct
       61 cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc
      121 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
      181 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
      241 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt
      301 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
      361 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
      421 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
      481 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
      541 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
      601 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
      661 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
      721 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccgttt
      781 atgtatcgag ctaggcactt aaatacaata tctctgcaat gcggcaattc agtggttcgt
      841 ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta ccaccttact
      901 tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg ctaactcatc
      961 gttaccatgt tgcaagacta caagagtatt gcataagact acattaagct tgcagctcca
     1021 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgc tgcaggcatg
     1081 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1141 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1261 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1441 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1621 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1681 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1741 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1801 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1861 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1921 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1981 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2221 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2281 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2341 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2401 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2461 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2521 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2581 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2641 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2701 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2761 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2821 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2881 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2941 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3001 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3061 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3241 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3301 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     3361 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     3421 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     3481 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     3541 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     3601 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     3661 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     3721 tcgagctcgg tacccgggga gcgcgcgtaa tacgactcac tatagggcga attgggtacg
     3781 tccctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     3841 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     3901 tgggcggagt taggggcggg atgggcggag ttaggggcgg gatagctaga gccagacatg
     3961 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     4021 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4081 gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
     4141 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
     4201 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
     4261 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
     4321 taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgaattag
     4381 cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt
     4441 cattggtctt aaaggtaccg atgcatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg
     4501 gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc
     4561 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg
     4621 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg
     4681 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc
     4741 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg
     4801 cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc
     4861 ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac
     4921 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac
     4981 ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc
     5041 ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg
     5101 cttgtactcg gtcatggtaa gctccggtgg atccccctgg ggagagaggt cggtgattcg
     5161 gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc ttgcagaatg cggaacaccg
     5221 cgcgggcagg aacagggccc acactaccgc cccacacccc gcctcccgca ccgccccttc
     5281 ccggccgctg ctctcggcgc gccctgctga gcagccgcta ttggccacag cccatcgcgg
     5341 tcggcgcgct gccattgctc cctggcgctg tccgtctgcg agggtactag tgagacgtgc
     5401 ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag gaaccttccc gacttagggg
     5461 cggagcagga agcgtcgccg gggggcccac aagggtagcg gcgaagatcc gggtgacgct
     5521 gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg ccgctgcgtt tcccggaacc
     5581 acgcccagag cagccgcgtc cctgcgcaaa cccagggctg ccttggaaaa ggcgcaaccc
     5641 caaccccctc gagccgcggc caaagtggat ctctgctgtc cctgtaataa acccgaaaat
     5701 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
     5761 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga
     5821 tgaatactgc catttgtctc gaggtcgaga attctctaga gaagcttgag ctcgagatct
     5881 gagtccggct agtactcttc accctcctcc tccccctcac cctccacaga atccacccca
     5941 acttcttcgt aatctttttc aagagctgcc atgtcttctc gtgcctcgct aaactcaccc
     6001 tcttccatcc cctcccctac ataccagtgc acaaacgccc ttttggcata catcaaatca
     6061 aatttatggt caaggcgggc ccaagcttca gcaatagccg tcgtgttact aagcatgcag
     6121 actgcgcgct gaacctttgc caagtcgcca cctggcacta ccgtcggagg ttggtaattt
     6181 attccgacct taaaccccgt tggacaccag tctacaaatt ggattgagcg cttagtctta
     6241 atggttgcta tggcggcgtt gacgtctttt ggtacaacgt cgccgcggta caacaagcaa
     6301 caggccatat atttaccatg cctggggtca cacttgacca tctggttcgc cggttcgaaa
     6361 cacgcattag ttatttccgc gacgctgagt tgctcgtggt atgctttctc tgcagagatg
     6421 acgggggcgt atgtagcaag tggaaagtgg atcctcgggt aggggaccaa atttgtctgg
     6481 aattctgtga ggtctacgtt gagtgcaccg tcgaatctaa gggaggcggt gatagaggag
     6541 actatctggg atatcagcct attgaggttt gtgtaagtcg gtcgttcgat gtcgagattg
     6601 cgcctgcata tatcataaat ggcttcatta tctaccatga aggcacagtc agaatgttca
     6661 agcgtagtgt gagtcgtaag tatactgtta tatggctcta cgactgcagt agatacctgc
     6721 ggggccggat agatgctgaa ttccaattta gatttcttcc cataatcaac gctgagccgc
     6781 tccatgagca aagacgtaaa acctgaccca gtacctcccc cgaaactatg gaacaccagg
     6841 aaaccctgga gccctgtaca ctgatcggcc agcttccgga ttcgatccag aaccaggtcg
     6901 attatttctt tgccgatggt ataatgacct ctcgcgtagt tattcgcagc atcctctttt
     6961 ccggttatca gttgttcggg atggaaaagt tgtctgtaag tgccggttcg aacttcatcg
     7021 atcactgttg gctccaagtc aacaaacacc gctcgcggga catgcttacc agcccccgtc
     7081 tcggaaaaga atgtgttaaa agaatcgtcg cctcctccga tggtcttatc agatggcatt
     7141 tgtccatccg gttggattcc atgctcaagg cagtaaagct cccagcaagc attgcctatt
     7201 tgtacgcctg cctgacctac gtggatactg atgcactccc gcatggatcc gctagcgtaa
     7261 tccggaacat cgtatgggta catggtggcg accggtagcg ctagcggatc tgacggttca
     7321 ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     7381 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     7441 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     7501 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     7561 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     7621 atttaccgtc attgacgtca atagggggcg tacttggcat atgatagtcc attttaaaac
     7681 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
     7741 ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca gccttgtatc
     7801 gtatatgcaa atatgaagga atcatgggaa ataggccctc ggtgaagggg gcggccgctc
     7861 gaggctagtc tcgtgatcga taccgtcgag atccgttcac taatcgaatg gatctgtctc
     7921 tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg
     7981 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa
     8041 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta
     8101 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt
     8161 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta
     8221 ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa
     8281 tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga
     8341 gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag
     8401 ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt
     8461 gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc
     8521 tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga
     8581 acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct
     8641 ttgccttggt gggtgctact cctaatggt
//
