LOCUS       sequence-44999-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(1113-1316)
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(779..797)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      793..840
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattccctc tgaatatttt gtttacttta agaaggagat ataccatggg cagcagccat
       61 catcatcatc atcacagcag cggcctggtg ccgcgcggca gccatatgta tgaagaagta
      121 gttcagactg ttaatacaga tttctctcca tatctgattt cagataactt agaacagcct
      181 atgggaagta gtcatgcatc tcaggtttgt tctgagacac ctgatgacct gttagatgat
      241 ggtgaaataa aggaagatac tagttttgct gaaaatgaca ttaaggaaag ttctgctgtt
      301 tttagcaaaa gcgtccagaa aggagagctt agcaggagtc ctagcccttt cacccataca
      361 catttggctc agggttaccg aagaggggcc aagaaattag agtcctcaga agagaactta
      421 tctagtgagg atgaagagct tccctgcttc caacacttgt tatttggtaa agtaaacaat
      481 ataccttctc agtctactag gcatagcacc gttgctaccg agtgtctgtc taagaacaca
      541 gaggagaatt tattatcatt gaagaatagc ttaaatgact gcagtaacca ggtaatattg
      601 gcaaaggcat ctcaggaaca tcaccttagt gaggaaacaa aatgttctgc tagcttgttt
      661 tcttcacagt gcagtgaatt ggaagacttg actgcaaata caaacaccca ggatcctttc
      721 taactcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctngcacc
      781 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      841 ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca
      901 agcctatgcc tacagcatcc acggtgacgg tgccgaggat gacaac
//
