LOCUS       sequence-449998-       11903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for PEMAX** protein in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11903)
  AUTHORS   Liu P, Ponnienselvan K, Nyalile T, Oikemus S, Joynt AT, Iyer S, 
            Kelly K, Guo D, Kyawe PP, Vanderleeden E, Redick SD, Huang L, Chen 
            Z, Lee JM, Schiffer CA, Harlan DM, Wang JP, Emerson CP Jr, Lawson 
            ND, Watts JK, Sontheimer EJ, Luban J, Wolfe SA
  TITLE     Increasing intracellular dNTP levels improves prime editing 
            efficiency.
  JOURNAL   Nat Biotechnol. 2024 Sep 25. doi: 10.1038/s41587-024-02405-x.
  PUBMED    39322763
REFERENCE   2  (bases 1 to 11903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Sep 25. doi: 10.1038/s41587-024-02405-x."
COMMENT     Sequence Label: PEmax_2star_pet21A
FEATURES             Location/Qualifiers
     source          1..11903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     660..679
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(699..776)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     982..1001
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1085..1104
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1104..1128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1143..1165
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1173..1205
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             1215..1241
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             1287..1307
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1308..5408
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5451..5477
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5499..5519
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7632..7652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7665..7712
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7719..7736
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7789..7807)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7803..7850
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7887..8342
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7974..7993)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8184..8205
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8368..8472
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8473..8541,8542..9333)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8691..8710)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9504..10092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9993..10012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10246..10263
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10278..10417
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10343..10362)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10503..10525
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10519..10710)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(11519..11903,1..698))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
       61 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
      121 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
      181 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
      241 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
      301 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
      361 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
      421 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
      481 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
      541 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
      601 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
      661 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
      721 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
      781 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
      841 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
      901 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
      961 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     1021 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     1081 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     1141 attttgttta actttaagaa ggagatatac atatggctag catgactggt ggacagcaaa
     1201 tgggtcgcgc cgctccggca gctaagaaaa agaaactgga tggcagcgtc gacaaacgga
     1261 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca
     1321 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     1381 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     1441 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     1501 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1561 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1621 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1681 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1741 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1801 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1861 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1921 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagaaagctg gaaaatctga
     1981 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     2041 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     2101 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     2161 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     2221 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     2281 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     2341 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     2401 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     2461 ccgaggaact gctcgtgaag ctgaagagag aggacctgct gcggaagcag cggaccttcg
     2521 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2581 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2641 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2701 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2761 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2821 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2881 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2941 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     3001 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     3061 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     3121 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     3181 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     3241 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     3301 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     3361 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     3421 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     3481 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     3541 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     3601 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     3661 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3721 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3781 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     3841 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3901 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3961 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     4021 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     4081 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     4141 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     4201 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     4261 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     4321 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     4381 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     4441 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     4501 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     4561 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     4621 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     4681 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4741 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4801 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4861 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4921 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4981 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     5041 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     5101 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     5161 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     5221 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     5281 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     5341 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     5401 gaggtgactc cggcggaagc tctggtggca gcaagcggac cgccggctct tacccatacg
     5461 atgttccaga ttacgctgac ggctctgaat tcgagagccc taagaagaaa agaaaggtga
     5521 gcggaggctc tagcggcgga agcaccctga acattgaaga cgagtataga ctgcatgaaa
     5581 caagcaagga acccgacgtg tccctgggct ccacctggct gtccgacttt ccccaggcct
     5641 gggccgagac aggaggaatg ggcctggccg tgcggcaggc acccctgatc atccctctga
     5701 aggccacctc tacacccgtg agcatcaagc agtaccctat gtctcaggag gccagactgg
     5761 gcatcaagcc tcacatccag aggctgctgg accagggcat cctggtgcca tgccagagcc
     5821 cctggaacac accactgctg cccgtgaaga agccaggcac caatgactat agacccgtgc
     5881 aggatctgag agaggtgaac aagagggtgg aggatatcca ccccaccgtg cccaaccctt
     5941 acaatctgct gtccggcctg cccccttctc accagtggta tacagtgctg gacctgaagg
     6001 atgccttctt ttgtctgaga ctgcacccta ccagccagcc actgttcgcc tttgagtgga
     6061 gggaccctga gatgggcatc tctggccagc tgacctggac acgcctgcct cagggcttca
     6121 agaatagccc aacactgttt aacgaggccc tgcaccgcga cctggcagat ttccggatcc
     6181 agcacccaga tctgatcctg ctgcagtaca tggacgatct gctgctggcc gccaccagcg
     6241 agctggattg ccagcaggga acacgcgccc tgctgcagac cctgggaaac ctgggatata
     6301 gggcatccgc caagaaggcc cagatctgtc agaagcaggt gaagtacctg ggctatctgc
     6361 tgaaggaggg ccagagatgg ctgacagagg ccaggaagga gacagtgatg ggccagccaa
     6421 cacccaagac cccaagacag ctgagggagt tcctgggcaa agcaggattt tgcaggctgt
     6481 tcatcccagg attcgcagag atggcagcac ctctgtaccc actgaccaag ccgggcaccc
     6541 tgtttaattg gggccctgac cagcagaagg cctatcagga gatcaagcag gccctgctga
     6601 cagcaccagc cctgggcctg ccagacctga ccaagccttt cgagctgttt gtggatgaga
     6661 agcagggcta cgccaagggc gtgctgaccc agaagctggg accatggaga cggcccgtgg
     6721 cctatctgtc caagaagctg gacccagtgg cagcaggatg gccaccatgc ctgaggatgg
     6781 tggcagcaat cgccgtgctg acaaaggatg ccggcaagct gaccatggga cagccactgg
     6841 tcatcaaggc accacacgca gtggaggccc tggtgaagca gcctccagat cgctggctgt
     6901 ctaacgcccg gatgacacac taccaggccc tgctgctgga caccgatcgc gtgcagtttg
     6961 gccctgtggt ggccctgaat ccagccaccc tgctgcctct gccagaggag ggcctgcagc
     7021 acaactgtct ggacatcctg gcagaggcac acggaacaag gccagacctg accgatcagc
     7081 ccctgcctga cgccgatcac acatggtata ccgatggaag ctccctgctg caggagggcc
     7141 agaggaaggc aggagcagca gtgaccacag agacagaagt gatctgggcc aaggccctgc
     7201 cagcaggcac atccgcccag cgggccgagc tgatcgccct gacccaggcc ctgaagatgg
     7261 ccgagggcaa gaagctgaac gtgtacacag actccagata tgccttcgcc accgcacaca
     7321 tccacggaga gatctacagg cgccggggct ggctgacctc tgagggcaag gagatcaaga
     7381 acaaggatga gatcctggcc ctgctgaagg ccctgtttct gcccaagcgg ctgagcatca
     7441 tccactgtcc tggacaccag aagggacact ccgccgaggc aaggggcaat cggatggccg
     7501 accaggccgc cagaaaggct gctattactg aaactcccga cacttccact ctgctgattg
     7561 aaaactcctc cccttctggc ggctcaaccg gtggtggtcc cgggggtggt gcggccgcag
     7621 gcagcggaag ccctaagaaa aaacgaaaag ttggcagcgg aagcaaaagg ccggcggcca
     7681 cgaaaaaggc cggccaggca aaaaagaaaa agctcgagca ccaccaccac caccactgag
     7741 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     7801 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     7861 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7921 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7981 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8041 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8101 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8161 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8221 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8281 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8341 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8401 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8461 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8521 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8581 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8641 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8701 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8761 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8821 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8881 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8941 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9001 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9061 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9121 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9181 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9241 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9301 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9361 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9421 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9481 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9541 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9601 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     9661 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9721 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9781 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9841 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9901 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9961 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10021 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10081 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10141 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10201 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10261 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
    10321 caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
    10381 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
    10441 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
    10501 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
    10561 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
    10621 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
    10681 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
    10741 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
    10801 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
    10861 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
    10921 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
    10981 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
    11041 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
    11101 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
    11161 gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
    11221 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
    11281 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
    11341 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
    11401 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
    11461 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
    11521 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    11581 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
    11641 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
    11701 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
    11761 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
    11821 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
    11881 cagtgggaac gatgccctca ttc
//
