LOCUS       sequence-45000-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(1234-1443)
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(797..815)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggtaa acaatatacc
      121 ttctcagtct actaggcata gcaccgttgc taccgagtgt ctgtctaaga acacagagga
      181 gaatttatta tcattgaaga atagcttaaa tgactgcagt aaccaggtaa tattggcaaa
      241 ggcatctcag gaacatcacc ttagtgagga aacaaaatgt tctgctagct tgttttcttc
      301 acagtgcagt gaattggaag acttgactgc aaatacaaac acccaggatc ctttcttgat
      361 tggttcttcc aaacaaatga ggcatcagtc tgaaagccag ggagttggtc tgagtgacaa
      421 ggaattggtt tcagatgatg aagaaagagg aacgggcttg gaagaaaata atcaagaaga
      481 gcaaagcatg gattcaaact taggtgaagc agcatctggg tgtgagagtg aaacaagcgt
      541 ctctgaagac tgctcagggc tatcctctca gagtgacatt ttaaccactc agcagaggga
      601 taccatgcaa cataacctga taaagctcca gcaggaaatg gctgaactag aagctgtgtt
      661 agaacagcat gggagccagc cttctaacag ctacccttcc atcataagtg actcttctgc
      721 ccttgaggac ctgcgataac tcgaggatcc ggctgctaac aaagccccga aaggaagcct
      781 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaacggg
      841 tcttgagggg tttttgctga aaggaggaac ccatatccgg atatcccgca agaggcccgc
      901 cagtaccggc ataacc
//
