LOCUS       sequence-45001-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(1316-1538)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(820..838)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatgttct tgattggttc ttccaaacaa
      121 atgaggcatc agtctgaaag ccagggagtt ggtctgagtg acaaggaatt ggtttcagat
      181 gatgaagaaa gaggaacggg cttggaagaa aataatcaag aagagcaaag catggattca
      241 aacttaggtg aagcagcatc tgggtgtgag agtgaaacaa gcgtctctga agactgctca
      301 gggctatcct ctcagagtga cattttaacc actcagcaga gggataccat gcaacataac
      361 ctgataaagc tccagcagga aatggctgaa ctagaagctg tgttagaaca gcatgggagc
      421 cagccttcta acagctaccc ttccatcata agtgactctt ctgcccttga ggacctgcga
      481 aatccagaac aaagcacatc agaaaaagca gtattaactt cacagaaaag tagtgaatac
      541 cctataagcc agaatccaga aggcctttct gctgacaagt ttgaggtgtc tgcagatagt
      601 tctaccagta aaaataaaga accaggagtg gaaaggtcat ccccttctaa atgcccatca
      661 ttagatgata ggtggtacat gcacagttgc tctgggagtc ttcagaatag aaactaccca
      721 tctcaagagg agctcattaa ggttgttgat gtggaggagc aacagttaag gatccggctg
      781 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      841 aaccccttgg gg
//
