LOCUS       sequence-45004-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(1443-1649)
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(771..789)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      785..832
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatgcgaa atccagaaca aagcacatca
      121 gaaaaagcag tattaacttc acagaaaagt agtgaatacc ctataagcca gaatccagaa
      181 ggcctttctg ctgacaagtt tgaggtgtct gcagatagtt ctaccagtaa aaataaagaa
      241 ccaggagtgg aaaggtcatc cccttctaaa tgcccatcat tagatgatag gtggtacatg
      301 cacagttgct ctgggagtct tcagaataga aactacccat ctcaagagga gctcattaag
      361 gttgttgatg tggaggagca acagctggaa gagtctgggc cacacgattt gacggaaaca
      421 tcttacttgc caaggcaaga tctagaggga accccttacc tggaatctgg aatcagcctc
      481 ttctctgatg accctgaatc tgatccttct gaagacagag ccccagagtc agctcgagtt
      541 ggcaacatac catcttcaac ctctgcattg aaagttcccc aattgaaagt tgcagaatct
      601 gcccagagtc cagctgctgc tcatactact gatactgctg ggtataatgc aatggaagaa
      661 agtgtgagca gggagaagcc agaattgaca gcttcaacag aaagggtcaa caaaagataa
      721 ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      781 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      841 aggaactata tccggatatc ccgcaagagg cccggcagta ccggcataac cagcctatgc
      901 tcactcagtg
//
