LOCUS       sequence-450055-       13185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of ApyA peptide modified by the B12-rSAM enzyme ApyD 
            (optional, refer to publication), the cytochrome P450 enzyme ApyO, 
            and the MNIO enzyme ApyHI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Nguyen DT, Zhu L, Gray DL, Woods TJ, Padhi C, Flatt KM, Mitchell DA,
            van der Donk WA
  TITLE     Biosynthesis of Macrocyclic Peptides with C-Terminal 
            beta-Amino-alpha-keto Acid Groups by Three Different Metalloenzymes.
  JOURNAL   ACS Cent Sci. 2024 Apr 11;10(5):1022-1032. doi: 
            10.1021/acscentsci.4c00088. eCollection 2024 May 22.
  PUBMED    38799663
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.4c00088"; journalName: "ACS Cent Sci"; date: 
            "2024-04-11- 11"; volume: "10"; issue: "5"; pages: "1022-1032"
COMMENT     Sequence Label: pSCrhaB2_ApyOAHID
FEATURES             Location/Qualifiers
     source          1..13185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..119
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             1704..1721
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5894..5911)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6064..6150
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6242..6269
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(6735..7397)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      7398..8169
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     primer_bind     9427..9445
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9562..9664
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(9731..9750)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(10620..10639)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(10663..10899)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(join(11225..11230,11231..11247,11248..11253))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             complement(11282..12130)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(12204..13040)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
       61 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa
      121 tgaaattcag caggatcaca taatgaacgg tctacctccg tcgcttccgc gggtcgacag
      181 caccgagtcg ctttttgcgg agccgctcgc gtttctcgca caagcgcgct cgcggcatgg
      241 tgatgtcttc gtcatgcggg agcatggtcc gattttttcc cgcgcgagcg attgcagcgg
      301 cgtgatagcg gtattcggcg agcatcggct tcgacagata ttgaccgata tagacaattt
      361 cgcgttgccg atgtccgcgg cggcgaaaat ggcattgccg aaaaacctcg tcaacctcaa
      421 tcgcggcttg cacagcatgc gcgagccgga gcatggccgg cacaagcgcc tcttgacggg
      481 aacgatcaat cgcgagttgt tcgacgcgca ccgcttcgaa atacgagcgg ctttgaaccg
      541 cttttgcgaa atgctgaaag tggaccgccg gatttcagtg gtcagccgga tgcgcgagct
      601 gaccgtggag atggcgtctc atatctttct tggggcgcag tgccaagagg atgacgaact
      661 ggcgtttctt ttaagcgcct attttacttt gcgacgcgaa gcgtcttcgc tgaacgcgcg
      721 cgatcccctg ctgtaccggg acgaactgat cggcgtcggg cagcaactcg accgcacatt
      781 gcgggagcgg atcaggcggt atcgaaagcg tcccgtcgac gctcgtgcgg ggctgctgca
      841 acgtctggca acggccggac cgccgggctc gcccgcgctt tccgaggacg agatagtcgg
      901 ccatgccaac gtgatgttcg tgtcgagcac cgagcccgtc gcgatgtcgc tgacgtggct
      961 gctgctcgtt ctgtcgcagc tgcccgatct gaggcgcgcg cttcgcgcgg aaattgccga
     1021 tcgcgcgtcg atgccggcat cgacgaacgg cgcgtcgtgg ctcgagaacg tcgtcaacga
     1081 gacgctccgg ctcctgacgc ccaacgcgct gatggttcgc gcgacgacgc gcgcggtctc
     1141 gctgcaaggc gtcgcgcttc cggcgcgttg cgagatcgtc gtgtgtccgt tcctcgcgca
     1201 tcgcgaagcc aagccgtttc cggacccgca tgcattctcg ccgtcccggt gggagacggc
     1261 caggccgtct ccgtacgagt attttccgtt cggcgccggc ggtcattttt gcgcggggcg
     1321 aaatctcgcg ctctcgctga ttcgcgaagt actgtcaacg ctgctatcgc gcttcgattt
     1381 cgttttggat ggcgaacagt ctatcgactg gcgcattcat atcatgttga tgccgaaagg
     1441 cgacccggca ctcatcgcgc atcccgtcga tgagcgcggt gacacgccgt cgccgaaatg
     1501 gcgcgggccg ataaccgatc tattccattt cgcgccgggg ctttcttgac atttaccagg
     1561 gcgcatcctt taatcagaag cgaatcggca tgcatgccca tcgggatttt ttccagtcat
     1621 tgatccgcat ggttcttttc acgggttatt ccgcgctcac acgctaagcg gcgcgtcctg
     1681 cctacaacaa aggaacaatc atgcatcacc atcaccatca catggctacg aaacccaaaa
     1741 aaacaaaggg cgtttctgtt tccatcgaag ggaagctgcc caagatgacc ctggatatgc
     1801 cggtggacgc gaaaaagatc aaggccatcc agaaatgcct ggaaaacggc aagctgacca
     1861 tcacgatgag caaggtggat cttgccggcg gccgcatggg cgagggctat ctctacgact
     1921 gatgtccggc ggcgctctgg ttttcgccgg cggcgtcgcg atctcgccgc ctcgatgctc
     1981 gggcctcgga gggaacgtgc cgccgaacgc gtgcgtttgg cgcgcggtcg cgcgataacg
     2041 ggcgatgaat tccgggcttc ggcagttcga ccttaagggc gcgggaagcc tatgaaagcc
     2101 ttcgatcgcg atgcatcgga gcctcggatc ggtctcgcgt atgggcccgg gatggcggaa
     2161 ttcgccgcac ggaacgcgca cctcgtcgat tacatcgaag tgccgttcga gcaattgcga
     2221 ttctcgccgg cggtcgccga gcttcagcaa accattccgt tcgttctgca ctgcgccagc
     2281 ttgtccgtcg cgggattcgt gccgcccgac gattcgacgg tcgacgcaat cgagcgcacg
     2341 gccgtgcaga ccggcacgcc atggataggc gaacatctcg cgtacatttc ggccgatccc
     2401 gttggcgaag cgctgggcgg gaccggtgag cccacctcgt tgtcgtacac gttgtgccct
     2461 cagttgagcg acgagacggt acgccgcgtc gtcgacaatc tcgccgcgct tcgtccgcat
     2521 tttccggttc cactgatcgt cgaaaactcc ccgcagtact ttccgatacc gggcagcacg
     2581 atgggaatga cggacttcat tcgcgcgatc acggaccgat gcgacgtcgg tttgctcctg
     2641 gacctgagtc acttcctgat cacggcgcac aacacgggcg cagaggtgca tcgggaactc
     2701 gcccggcttc cgctggagcg cgtggtcgaa gttcatctgt ccggaatgag cgtgcagtcc
     2761 ggcacggcct gggacgacca ttcgctgccg gcatcgccga tcctgttcga actgctcgag
     2821 cgcctgctcg acgtggcgcg gcctcgcgcg ctgaccttcg aatacaactg gtcgccatac
     2881 ttcccgctgt cggtcctgac gacgcatatc gagcgcgcac gccagttgat ggggctcgca
     2941 tgaacgcagg gaaaatacac gccgtgatcg ccgccggcgc caaggacccg cggctcatcg
     3001 agcattggcg gcggcgtccg gacctgctgc gccgcgcagg aatcgagccg gatcatctcg
     3061 atttgcgcgt attggagaat ttcgccgggt tgagcctgaa ggtgcgtcac aacggcctgc
     3121 gcggggatct gccgctgagt ttcaggttgt tgagcgtggc cggcctcgag atcgaggtct
     3181 tcgccgcgta tgcgtcggac tgcgcggaaa gcggccatgt attcgccgcc acgccgcggg
     3241 aacgcgcgcg cgatctgatg gcgttcctcg acagatggct ggaccccgcg caacgaaatc
     3301 acgttctgct gtgggacatc cttcgtcatg aatatgcgct ttcgtgcctg cggcaagaaa
     3361 gcgccgcgcc gctacccgcc gctcgccgca tcatccgcca tcgggatcgc gcgacaggcg
     3421 gctgcattcc gatgctcaac ggctccgtcg ctcagcacgt gatgcattgc aatccggaag
     3481 acctggcgtc ggcgctgcgt cagaaggagc cgccgctcga tgcggttccc gccgccgaca
     3541 cttactgctg ctattggcgc tccgccgaaa cgggcgaaat ccggtttctg caggccgata
     3601 cgctcggcta tttcgcgatc gaagcgatcg acggccggcg atccgccgcc gacatctgcg
     3661 atcggctgct cggcggccgc cgtctgctgc cgcagttcct ccggtcgctc gaccaactgg
     3721 ccggaatcgg gatcgtgcgg ttcgagaggc cgctcggaag cgctgcggca tgaaactgct
     3781 gctcgtcgac aacctcgtca tgccggaaga gggctcgctc gcgtacctcg acgtgcatcc
     3841 gcatctcggg ctgctcgcgc tggccgcggt cgccgaagcc gacggccatc gcgtgcagat
     3901 ctacgatccg aaacgactga tcagggacgg cacgctggct tacgatgcga cgctttacga
     3961 gcgggcgagc caagcgattc tcgcggcgcg tccggacggg gtcggcttca cggcgctcgg
     4021 ctgcagcttt ctgtttgcgc tcaatgtcgc ggcgctgttg aagcggcgcg agcccgacct
     4081 gcccgtcttg ctcggcgggc cgcacgcgac gatgctgcac cggcagatcc tcgagcggtt
     4141 cccgcagttc gacatcatcg tccggtacga ggcggatgaa atcctgcccg ccgtgctcga
     4201 ctgcctcccg caccggacat tcgacgtgat tcccggcttg agctggcgcg cgacgggccg
     4261 cggctcgccg ttgcgcttca ccgacggcaa gcccaaggtc gaggatctgg acctgctgcc
     4321 gatcgcctcg tacgaccatt atccggttga ggaactgggg ctctcgatgc tgcgcatcga
     4381 ggccggccga gggtgtccgt tcgcatgcac attctgctcg acggccggtt tctttcaacg
     4441 cagcttccgc ctcaaatcgg ccgagcgcct ggttcgcgaa ctggatatcc ttcaccagcg
     4501 ttatcgcgtg tcggacttca agctcgacca cgacatgttc accgtgaatc gccgcaaggt
     4561 aatggagttt tgcgaggcgg tcgccgggcg cgactatcgg tggcgcgcgt cggcgcggat
     4621 cgactgcgtc gacgaagcat tgttgaagaa gatggccgac gcgggctgcg tgaatcttta
     4681 tttcggcgtg gagacgggct ccgagcgcat gcaaaagctc tgcaagaagc ggctggatct
     4741 gcaacgggtc gagcccattc tggcggcggc ggattcattc ggaatcgaga cgaccgcgtc
     4801 gttcatcacc ggctatcccg aggaaaccgg ggaggatcag gacgacacgc tggacatgat
     4861 cgggcgctgc gctcggcgag cgtcttgcct cacgcaactg cacatgctcg cgccggagcc
     4921 cggcacaccg ctgttcgacg aacgcggcgc ggaaatcgcc tacgacggct gtggcgggcc
     4981 ttacaacacg cgcctgctga gttcgtcgga cgagcgtgcg gtgctcggcc atcccgacat
     5041 attccaaacc tactatcact accccgccgc gctgccgcgt gcgcgataca tctttgccgt
     5101 cggggcggcg gatctgctgc gccgggtcgg gccggtcatt ttcgcgtatg cgttgcgggg
     5161 gttcggaggg cggctgagca cgctgatatc ggcgtggcga caattcgccg acgaaacgtc
     5221 gcccggcgcg cccgcggacg cggcggggct cgaggccttc attgccgcgc ggttcggccg
     5281 cggccatcac ctgcattctc tttttcggta cgcgttgcgg ctctatgcgg cccgcgctcc
     5341 ggcggacgta atcgtcgagg ggccctatga gccggaccgg ccttgcatgc tggccgaaaa
     5401 cgtcggtctg ctggaggaca ttcacaattg cgacgcattg gtcgaccgga tcaggcgatc
     5461 gaccgaaggc gcgccgatgc tcggcgacgc ggatgtcggc gggctcggaa catatatcgt
     5521 tcaggggcac ggcgaggccg ccacgggcta ttgggtggag tccggcatcg ctacgcttct
     5581 cgatctgttc aggtcgcccc gttcgtgccg cgccgttgcg caatggctgg cggacgccac
     5641 ccgcaatgac ggcatagacg cttcgatctt cgagccgctg ctgcgcagag gcatcctggt
     5701 gtcggccggt taaatccatt tcgtccatgg tgcgtcgcac actcgaacgt tggtccgcgc
     5761 tggatctcgc ggaagggctt caactggcgc atgccgtcgc cgcgctgcag gcgctcggcg
     5821 ttctggacta gtctagagtc gacctgcagg catgcaagct tggctgtttt ggcggatgag
     5881 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     5941 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     6001 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
     6061 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     6121 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     6181 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
     6241 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttgt tattggtcga
     6301 cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     6361 gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc
     6421 tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat
     6481 gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcatt
     6541 tattctgcct cccagagcct gataaaaacg gtgaatccgt tagcgaggtg ccgccggctt
     6601 ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa
     6661 ggtatagggc ggcgaggcgg ctacagccga tagtctggaa cagcgcactt acgggttgct
     6721 gcgcaaccca agtgctaccg gcgcggcagc gtgacccgtg tcggcggctc caacggctcg
     6781 ccatcgtcca gaaaacacgg ctcatcgggc atcggcaggc gctgctgccc gcgccgttcc
     6841 cattcctccg tttcggtcaa ggctggcagg tctggttcca tgcccggaat gccgggctgg
     6901 ctgggcggct cctcgccggg gccggtcggt agttgctgct cgcccggata cagggtcggg
     6961 atgcggcgca ggtcgccatg ccccaacagc gattcgtcct ggtcgtcgtg atcaaccacc
     7021 acggcggcac tgaacaccga caggcgcaac tggtcgcggg gctggcccca cgccacgcgg
     7081 tcattgacca cgtaggccga cacggtgccg gggccgttga gcttcacgac ggagatccag
     7141 cgctcggcca ccaagtcctt gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc
     7201 ttggaaagtg tcttctggct gaccaccacg gcgttctggt ggcccatctg cgccacgagg
     7261 tgatgcagca gcattgccgc cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc
     7321 gctttgcgtt ccgtttgcac ccagtgaccg ggcttgttct tggcttgaat gccgatttct
     7381 ctggactgcg tggccatgct tatctccatg cggtaggggt gccgcacggt tgcggcacca
     7441 tgcgcaatca gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg
     7501 cagtcaatta cagattttct ttaacctacg caatgagcta ttgcgggggg tgccgcaatg
     7561 agctgttgcg tacccccctt ttttaagttg ttgattttta agtctttcgc atttcgccct
     7621 atatctagtt ctttggtgcc caaagaaggg cacccctgcg gggttccccc acgccttcgg
     7681 cgcggctccc cctccggcaa aaagtggccc ctccggggct tgttgatcga ctgcgcggcc
     7741 ttcggccttg cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc
     7801 tcggggggca ggcgggcggg cttcgccctt cgactgcccc cactcgcata ggcttgggtc
     7861 gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt
     7921 ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc aggccggagg cttttcccca
     7981 gagaaaatta aaaaaattga tggggcaagg ccgcaggccg cgcagttgga gccggtgggt
     8041 atgtggtcga aggctgggta gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg
     8101 cagcctgtcc atcagcttgt ccagcagggt tgtccacggg ccgagcgaag cgagccagcc
     8161 ggtggccgct cgcggccatc gtccacatat ccacgggctg gcaagggagc gcagcgaccg
     8221 cgcagggcga agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg
     8281 actttgcgaa gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc
     8341 gccttgcgct gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc
     8401 atacgcgatc atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa
     8461 gcacgcctac cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga
     8521 gcactgggcg gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga
     8581 gaagcggcgc aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg
     8641 gtggaagtcg gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct
     8701 ggcggacaag tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag
     8761 cccgcacatg accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga
     8821 gttcatcggc aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc
     8881 cgatctaggg ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc
     8941 gttctacgag gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt
     9001 cgagccacgc gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt
     9061 tgagacgccg gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc
     9121 tgccctacag gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga
     9181 gacggcccga gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa
     9241 tcgggatatg caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac
     9301 ggaacagcgg gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc
     9361 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9421 tataaaaata ggcgtatcac gaggcccttt cgtcttcgaa taaatacctg tgacggaaga
     9481 tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa
     9541 cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga
     9601 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
     9661 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     9721 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     9781 ggatattacg gcctttttag cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     9841 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     9901 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     9961 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
    10021 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
    10081 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcggcttt gttgaataaa
    10141 tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc
    10201 cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa
    10261 ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt atgagtcagc
    10321 aacaccttct tcacgaggca gacctcagcg ccagaaggcc gccagagagg ccgagcgcgg
    10381 ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct gctacgggcg
    10441 tctgacgcgg tggaaagggg gaggggatgt tgtttaaatg gctctgctgt agtgagtggt
    10501 ctacggggtc tgacgctcag tggaacgaaa tcgatgataa gctgtcaaac atgagcagat
    10561 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
    10621 ctatatcgcc gacatcaccg atgggagatc ctaagatatc gcttaggcca cacgttcaag
    10681 tgcagccaca ggataaattt gcactgagcc tgggtgggat tcggactcga ccgcatagcc
    10741 ttcaggagtg agttttgtgc aataccaacc gacgacttga ccctgccaag cggcaccaga
    10801 tttcttgcgt acgcgatccc ctaagccaaa ggtggcactc aggggaagcg caaactgccc
    10861 tgcaacggga gcgttggctt catcgctact ttgacccatg tcgaatcctt cttgtgaatc
    10921 tattatggcg acaagcaaat cgagctctga ctgcctaccc cacaacaact atcagaaagc
    10981 accagcacaa cggctgccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
    11041 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
    11101 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
    11161 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
    11221 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgcgagct catcgatgca
    11281 tttaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg gtttcccggg
    11341 taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa atatcactga
    11401 ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc tgtcgcagat
    11461 attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg cgctcactgc
    11521 acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta gccgccagcc
    11581 gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa tcactggtgt
    11641 aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc atttcgttag
    11701 caaacggcac atgctgacta ctttcatgct caagctgacc gataacctgc cgcgcctgcg
    11761 ccatccccat gctacctaag cgccagtgtg gttgccctgc gctggcgtta aatcccggaa
    11821 tcgccccctg ccagtcaaga ttcagcttca gacgctccgg gcaataaata atattctgca
    11881 aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg taaaagagat
    11941 cgccacgggt aatgcgataa gggcgatcgt tgagtacatg caggccatta ccgcgccaga
    12001 caatcaccag ctcacaaaaa tcatgtgtat gttcagcaaa gacatcttgc ggataacggt
    12061 cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga agttttaact
    12121 gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca atatggcgta
    12181 caaatacgtt gagaagattc gcgttattgc agaaagccat cccgtccctg gcgaatatca
    12241 cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact gtcgctgaat
    12301 ccacagcgat aggcgatgtc agtaacgctg gcctcgctgt ggcgtagcag atgtcgggct
    12361 ttcatcagtc gcaggcggtt caggtatcgc tgaggcgtca gtcccgtttg ctgcttaagc
    12421 tgccgatgta gcgtacgcag tgaaagagaa aattgatccg ccacggcatc ccaattcacc
    12481 tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga tgcgctgttt
    12541 tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat aaacaagatc
    12601 tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat ctgtgcaacc
    12661 agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgagacgg atactgccca
    12721 tccagctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg atccggcgag
    12781 cgatacagca cattggtcag acacagatta tcggtatgtt catacagatg ccgatcatga
    12841 tcgcgtacga aacagaccgt gccaccggtg atggtatagg gctgcccatt aaacacatga
    12901 atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg ttcaggaaaa
    12961 tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt taccagacgg aaaaaaatcc
    13021 acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga aatagtaatc
    13081 acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa cgtcgatttt
    13141 tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacat
//
