LOCUS       sequence-450057-       14260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of ApyA peptide modified by the B12-rSAM enzyme ApyD 
            (optional, refer to publication), the cytochrome P450 enzyme ApyO, 
            and the MNIO enzyme ApyHI, and the methyltransferase ApyS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14260)
  AUTHORS   Nguyen DT, Zhu L, Gray DL, Woods TJ, Padhi C, Flatt KM, Mitchell DA,
            van der Donk WA
  TITLE     Biosynthesis of Macrocyclic Peptides with C-Terminal 
            beta-Amino-alpha-keto Acid Groups by Three Different Metalloenzymes.
  JOURNAL   ACS Cent Sci. 2024 Apr 11;10(5):1022-1032. doi: 
            10.1021/acscentsci.4c00088. eCollection 2024 May 22.
  PUBMED    38799663
REFERENCE   2  (bases 1 to 14260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.4c00088"; journalName: "ACS Cent Sci"; date: 
            "2024-04-11- 11"; volume: "10"; issue: "5"; pages: "1022-1032"
COMMENT     Sequence Label: pSCrhaB2_ApyD_ RBS_ApyAOHIS
FEATURES             Location/Qualifiers
     source          1..14260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..119
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             3667..3684
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6969..6986)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7139..7225
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7317..7344
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(7810..8472)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      8473..9244
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     primer_bind     10502..10520
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10637..10739
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(10806..10825)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(11695..11714)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(11738..11974)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(join(12300..12305,12306..12322,12323..12328))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             complement(12357..13205)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(13279..14115)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
       61 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa
      121 tgaaattcag caggatcaca taatgaaact gctgctcgtc gacaacctcg tcatgccgga
      181 agagggctcg ctcgcgtacc tcgacgtgca tccgcatctc gggctgctcg cgctggccgc
      241 ggtcgccgaa gccgacggcc atcgcgtgca gatctacgat ccgaaacgac tgatcaggga
      301 cggcacgctg gcttacgatg cgacgcttta cgagcgggcg agccaagcga ttctcgcggc
      361 gcgtccggac ggggtcggct tcacggcgct cggctgcagc tttctgtttg cgctcaatgt
      421 cgcggcgctg ttgaagcggc gcgagcccga cctgcccgtc ttgctcggcg ggccgcacgc
      481 gacgatgctg caccggcaga tcctcgagcg gttcccgcag ttcgacatca tcgtccggta
      541 cgaggcggat gaaatcctgc ccgccgtgct cgactgcctc ccgcaccgga cattcgacgt
      601 gattcccggc ttgagctggc gcgcgacggg ccgcggctcg ccgttgcgct tcaccgacgg
      661 caagcccaag gtcgaggatc tggacctgct gccgatcgcc tcgtacgacc attatccggt
      721 tgaggaactg gggctctcga tgctgcgcat cgaggccggc cgagggtgtc cgttcgcatg
      781 cacattctgc tcgacggccg gtttctttca acgcagcttc cgcctcaaat cggccgagcg
      841 cctggttcgc gaactggata tccttcacca gcgttatcgc gtgtcggact tcaagctcga
      901 ccacgacatg ttcaccgtga atcgccgcaa ggtaatggag ttttgcgagg cggtcgccgg
      961 gcgcgactat cggtggcgcg cgtcggcgcg gatcgactgc gtcgacgaag cattgttgaa
     1021 gaagatggcc gacgcgggct gcgtgaatct ttatttcggc gtggagacgg gctccgagcg
     1081 catgcaaaag ctctgcaaga agcggctgga tctgcaacgg gtcgagccca ttctggcggc
     1141 ggcggattca ttcggaatcg agacgaccgc gtcgttcatc accggctatc ccgaggaaac
     1201 cggggaggat caggacgaca cgctggacat gatcgggcgc tgcgctcggc gagcgtcttg
     1261 cctcacgcaa ctgcacatgc tcgcgccgga gcccggcaca ccgctgttcg acgaacgcgg
     1321 cgcggaaatc gcctacgacg gctgtggcgg gccttacaac acgcgcctgc tgagttcgtc
     1381 ggacgagcgt gcggtgctcg gccatcccga catattccaa acctactatc actaccccgc
     1441 cgcgctgccg cgtgcgcgat acatctttgc cgtcggggcg gcggatctgc tgcgccgggt
     1501 cgggccggtc attttcgcgt atgcgttgcg ggggttcgga gggcggctga gcacgctgat
     1561 atcggcgtgg cgacaattcg ccgacgaaac gtcgcccggc gcgcccgcgg acgcggcggg
     1621 gctcgaggcc ttcattgccg cgcggttcgg ccgcggccat cacctgcatt ctctttttcg
     1681 gtacgcgttg cggctctatg cggcccgcgc tccggcggac gtaatcgtcg aggggcccta
     1741 tgagccggac cggccttgca tgctggccga aaacgtcggt ctgctggagg acattcacaa
     1801 ttgcgacgca ttggtcgacc ggatcaggcg atcgaccgaa ggcgcgccga tgctcggcga
     1861 cgcggatgtc ggcgggctcg gaacatatat cgttcagggg cacggcgagg ccgccacggg
     1921 ctattgggtg gagtccggca tcgctacgct tctcgatctg ttcaggtcgc cccgttcgtg
     1981 ccgcgccgtt gcgcaatggc tggcggacgc cacccgcaat gacggcatag acgcttcgat
     2041 cttcgagccg ctgctgcgca gaggcatcct ggtgtcggcc ggttaaatag caggaggaat
     2101 tcaccatgaa cggtctacct ccgtcgcttc cgcgggtcga cagcaccgag tcgctttttg
     2161 cggagccgct cgcgtttctc gcacaagcgc gctcgcggca tggtgatgtc ttcgtcatgc
     2221 gggagcatgg tccgattttt tcccgcgcga gcgattgcag cggcgtgata gcggtattcg
     2281 gcgagcatcg gcttcgacag atattgaccg atatagacaa tttcgcgttg ccgatgtccg
     2341 cggcggcgaa aatggcattg ccgaaaaacc tcgtcaacct caatcgcggc ttgcacagca
     2401 tgcgcgagcc ggagcatggc cggcacaagc gcctcttgac gggaacgatc aatcgcgagt
     2461 tgttcgacgc gcaccgcttc gaaatacgag cggctttgaa ccgcttttgc gaaatgctga
     2521 aagtggaccg ccggatttca gtggtcagcc ggatgcgcga gctgaccgtg gagatggcgt
     2581 ctcatatctt tcttggggcg cagtgccaag aggatgacga actggcgttt cttttaagcg
     2641 cctattttac tttgcgacgc gaagcgtctt cgctgaacgc gcgcgatccc ctgctgtacc
     2701 gggacgaact gatcggcgtc gggcagcaac tcgaccgcac attgcgggag cggatcaggc
     2761 ggtatcgaaa gcgtcccgtc gacgctcgtg cggggctgct gcaacgtctg gcaacggccg
     2821 gaccgccggg ctcgcccgcg ctttccgagg acgagatagt cggccatgcc aacgtgatgt
     2881 tcgtgtcgag caccgagccc gtcgcgatgt cgctgacgtg gctgctgctc gttctgtcgc
     2941 agctgcccga tctgaggcgc gcgcttcgcg cggaaattgc cgatcgcgcg tcgatgccgg
     3001 catcgacgaa cggcgcgtcg tggctcgaga acgtcgtcaa cgagacgctc cggctcctga
     3061 cgcccaacgc gctgatggtt cgcgcgacga cgcgcgcggt ctcgctgcaa ggcgtcgcgc
     3121 ttccggcgcg ttgcgagatc gtcgtgtgtc cgttcctcgc gcatcgcgaa gccaagccgt
     3181 ttccggaccc gcatgcattc tcgccgtccc ggtgggagac ggccaggccg tctccgtacg
     3241 agtattttcc gttcggcgcc ggcggtcatt tttgcgcggg gcgaaatctc gcgctctcgc
     3301 tgattcgcga agtactgtca acgctgctat cgcgcttcga tttcgttttg gatggcgaac
     3361 agtctatcga ctggcgcatt catatcatgt tgatgccgaa aggcgacccg gcactcatcg
     3421 cgcatcccgt cgatgagcgc ggtgacacgc cgtcgccgaa atggcgcggg ccgataaccg
     3481 atctattcca tttcgcgccg gggctttctt gacatttacc agggcgcatc ctttaatcag
     3541 aagcgaatcg gcatgcatgc ccatcgggat tttttccagt cattgatccg catggttctt
     3601 ttcacgggtt attccgcgct cacacgctaa gcggcgcgtc ctgcctacaa caaaggaaca
     3661 atcatgcatc accatcacca tcacatggct acgaaaccca aaaaaacaaa gggcgtttct
     3721 gtttccatcg aagggaagct gcccaagatg accctggata tgccggtgga cgcgaaaaag
     3781 atcaaggcca tccagaaatg cctggaaaac ggcaagctga ccatcacgat gagcaaggtg
     3841 gatcttgccg gcggccgcat gggcgagggc tatctctacg actgatgtcc ggcggcgctc
     3901 tggttttcgc cggcggcgtc gcgatctcgc cgcctcgatg ctcgggcctc ggagggaacg
     3961 tgccgccgaa cgcgtgcgtt tggcgcgcgg tcgcgcgata acgggcgatg aattccgggc
     4021 ttcggcagtt cgaccttaag ggcgcgggaa gcctatgaaa gccttcgatc gcgatgcatc
     4081 ggagcctcgg atcggtctcg cgtatgggcc cgggatggcg gaattcgccg cacggaacgc
     4141 gcacctcgtc gattacatcg aagtgccgtt cgagcaattg cgattctcgc cggcggtcgc
     4201 cgagcttcag caaaccattc cgttcgttct gcactgcgcc agcttgtccg tcgcgggatt
     4261 cgtgccgccc gacgattcga cggtcgacgc aatcgagcgc acggccgtgc agaccggcac
     4321 gccatggata ggcgaacatc tcgcgtacat ttcggccgat cccgttggcg aagcgctggg
     4381 cgggaccggt gagcccacct cgttgtcgta cacgttgtgc cctcagttga gcgacgagac
     4441 ggtacgccgc gtcgtcgaca atctcgccgc gcttcgtccg cattttccgg ttccactgat
     4501 cgtcgaaaac tccccgcagt actttccgat accgggcagc acgatgggaa tgacggactt
     4561 cattcgcgcg atcacggacc gatgcgacgt cggtttgctc ctggacctga gtcacttcct
     4621 gatcacggcg cacaacacgg gcgcagaggt gcatcgggaa ctcgcccggc ttccgctgga
     4681 gcgcgtggtc gaagttcatc tgtccggaat gagcgtgcag tccggcacgg cctgggacga
     4741 ccattcgctg ccggcatcgc cgatcctgtt cgaactgctc gagcgcctgc tcgacgtggc
     4801 gcggcctcgc gcgctgacct tcgaatacaa ctggtcgcca tacttcccgc tgtcggtcct
     4861 gacgacgcat atcgagcgcg cacgccagtt gatggggctc gcatgaacgc agggaaaata
     4921 cacgccgtga tcgccgccgg cgccaaggac ccgcggctca tcgagcattg gcggcggcgt
     4981 ccggacctgc tgcgccgcgc aggaatcgag ccggatcatc tcgatttgcg cgtattggag
     5041 aatttcgccg ggttgagcct gaaggtgcgt cacaacggcc tgcgcgggga tctgccgctg
     5101 agtttcaggt tgttgagcgt ggccggcctc gagatcgagg tcttcgccgc gtatgcgtcg
     5161 gactgcgcgg aaagcggcca tgtattcgcc gccacgccgc gggaacgcgc gcgcgatctg
     5221 atggcgttcc tcgacagatg gctggacccc gcgcaacgaa atcacgttct gctgtgggac
     5281 atccttcgtc atgaatatgc gctttcgtgc ctgcggcaag aaagcgccgc gccgctaccc
     5341 gccgctcgcc gcatcatccg ccatcgggat cgcgcgacag gcggctgcat tccgatgctc
     5401 aacggctccg tcgctcagca cgtgatgcat tgcaatccgg aagacctggc gtcggcgctg
     5461 cgtcagaagg agccgccgct cgatgcggtt cccgccgccg acacttactg ctgctattgg
     5521 cgctccgccg aaacgggcga aatccggttt ctgcaggccg atacgctcgg ctatttcgcg
     5581 atcgaagcga tcgacggccg gcgatccgcc gccgacatct gcgatcggct gctcggcggc
     5641 cgccgtctgc tgccgcagtt cctccggtcg ctcgaccaac tggccggaat cgggatcgtg
     5701 cggttcgaga ggccgctcgg aagcgctgcg gcatgaaact gctgctcgtc gacaacctcg
     5761 tcatgccgga agagggctcg ctcgcgtacc tcgacgtgca tgccgttgcg caatggctgg
     5821 cggacgccac ccgcaatgac ggcatagacg cttcgatctt cgagccgctg ctgcgcagag
     5881 gcatcctggt gtcggccggt taaatccatt tcgtccatgg tgcgtcgcac actcgaacgt
     5941 tggtccgcgc tggatctcgc ggaagggctt caactggcgc atgccgtcgc cgcgctgcag
     6001 gcgctcggcg ttctggacgc gatgagggag ccggtcacgg cggaaacgtt gagcgccagc
     6061 catgatctcg atcccgagtt gctgcgcggc atcctcgaat atgcggcgtc gagaacgaat
     6121 ctggttcgca agacggaccg cggattcgcg atttccgagc attacaccgc gcaagcgcgc
     6181 tttctgttga acctgtacgc cggcgcttat gccgataacg cttcacggct ggcgacgctg
     6241 ctccggcgtc cggcattggc gggctccatg atcgacctcg tcgcgcacgc gcgcgccttc
     6301 gacgcgagcg aggccggcgg tggagcgctg gcgtcgatcg tcacgcaatt gcgtctcaat
     6361 catgtgctgg atctgggatg cggcgccggc gcgctgctgc gcgagttggc ggcggacgat
     6421 cgcggattcg tcggctgggg actggatcgc aatcccgcga tgtgcaaggc ggcgcgggtt
     6481 cgagcgcggc aagcggggct cgccgcgcgc gtgaaggtgt tccacggcga cggctggaat
     6541 cccggggcgt cggtcccgcc gcgcgtgctg gcgcgcgtgc gcaacgtcgt cgcgagccag
     6601 ttcgtcaacg agatgtttcg cggcggcaca tcgcgagtgg aaacgtggct tcggcgcatg
     6661 cggcgcttgc tgcccggccg catgctcgtg atatcggact attacggccg cctcggccac
     6721 ggcactcaca cgatgcgccg cgagacgctg ctgcatgact atgtgcagct catatcgggg
     6781 cagggcgtgc cgccgcccga ttccgacgcg tggttggcca tgtatcgagc agccggttgc
     6841 cggcctctgc acgtgatcga agaccgggcg gccacgacat gcttcgtcca tctggtgggg
     6901 ctatagtcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag
     6961 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
     7021 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     7081 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
     7141 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     7201 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
     7261 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa
     7321 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttgttattg gtcgacttaa
     7381 acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag
     7441 caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg
     7501 ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg
     7561 aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc
     7621 tgcctcccag agcctgataa aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt
     7681 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     7741 agggcggcga ggcggctaca gccgatagtc tggaacagcg cacttacggg ttgctgcgca
     7801 acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc
     7861 gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc
     7921 ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg gctggctggg
     7981 cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg
     8041 gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc
     8101 ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca cgcggtcatt
     8161 gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga tccagcgctc
     8221 ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga tgagcttgga
     8281 aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg
     8341 cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct catgcgcttt
     8401 gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga tttctctgga
     8461 ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc acggttgcgg caccatgcgc
     8521 aatcagctgc aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc
     8581 aattacagat tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg
     8641 ttgcgtaccc ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc
     8701 tagttctttg gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg
     8761 ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg
     8821 ccttgcccaa ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg
     8881 gggcaggcgg gcgggcttcg cccttcgact gcccccactc gcataggctt gggtcgttcc
     8941 aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc gccttccact
     9001 tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt ccccagagaa
     9061 aattaaaaaa attgatgggg caaggccgca ggccgcgcag ttggagccgg tgggtatgtg
     9121 gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc aggcgcagcc
     9181 tgtccatcag cttgtccagc agggttgtcc acgggccgag cgaagcgagc cagccggtgg
     9241 ccgctcgcgg ccatcgtcca catatccacg ggctggcaag ggagcgcagc gaccgcgcag
     9301 ggcgaagccc ggagagcaag cccgtagggc gccgcagccg ccgtaggcgg tcacgacttt
     9361 gcgaagcaaa gtctagtgag tatactcaag cattgagtgg cccgccggag gcaccgcctt
     9421 gcgctgcccc cgtcgagccg gttggacacc aaaagggagg ggcaggcatg gcggcatacg
     9481 cgatcatgcg atgcaagaag ctggcgaaaa tgggcaacgt ggcggccagt ctcaagcacg
     9541 cctaccgcga gcgcgagacg cccaacgctg acgccagcag gacgccagag aacgagcact
     9601 gggcggccag cagcaccgat gaagcgatgg gccgactgcg cgagttgctg ccagagaagc
     9661 ggcgcaagga cgctgtgttg gcggtcgagt acgtcatgac ggccagcccg gaatggtgga
     9721 agtcggccag ccaagaacag caggcggcgt tcttcgagaa ggcgcacaag tggctggcgg
     9781 acaagtacgg ggcggatcgc atcgtgacgg ccagcatcca ccgtgacgaa accagcccgc
     9841 acatgaccgc gttcgtggtg ccgctgacgc aggacggcag gctgtcggcc aaggagttca
     9901 tcggcaacaa agcgcagatg acccgcgacc agaccacgtt tgcggccgct gtggccgatc
     9961 tagggctgca acggggcatc gagggcagca aggcacgtca cacgcgcatt caggcgttct
    10021 acgaggccct ggagcggcca ccagtgggcc acgtcaccat cagcccgcaa gcggtcgagc
    10081 cacgcgccta tgcaccgcag ggattggccg aaaagctggg aatctcaaag cgcgttgaga
    10141 cgccggaagc cgtggccgac cggctgacaa aagcggttcg gcaggggtat gagcctgccc
    10201 tacaggccgc cgcaggagcg cgtgagatgc gcaagaaggc cgatcaagcc caagagacgg
    10261 cccgagacct tcgggagcgc ctgaagcccg ttctggacgc cctggggccg ttgaatcggg
    10321 atatgcaggc caaggccgcc gcgatcatca aggccgtggg cgaaaagctg ctgacggaac
    10381 agcgggaagt ccagcgccag aaacaggccc agcgccagca ggaacgcggg cgcgcacatt
    10441 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10501 aaataggcgt atcacgaggc cctttcgtct tcgaataaat acctgtgacg gaagatcact
    10561 tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt
    10621 ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
    10681 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
    10741 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
    10801 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
    10861 ttacggcctt tttagctttt gccattctca ccggattcag tcgtcactca tggtgatttc
    10921 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
    10981 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
    11041 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
    11101 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
    11161 taacactggc agagcattac gctgacttga cgggacggcg gctttgttga ataaatcgaa
    11221 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
    11281 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
    11341 gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag tcagcaacac
    11401 cttcttcacg aggcagacct cagcgccaga aggccgccag agaggccgag cgcggccgtg
    11461 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
    11521 cgcggtggaa agggggaggg gatgttgttt aaatggctct gctgtagtga gtggtctacg
    11581 gggtctgacg ctcagtggaa cgaaatcgat gataagctgt caaacatgag cagatcctct
    11641 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
    11701 tcgccgacat caccgatggg agatcctaag atatcgctta ggccacacgt tcaagtgcag
    11761 ccacaggata aatttgcact gagcctgggt gggattcgga ctcgaccgca tagccttcag
    11821 gagtgagttt tgtgcaatac caaccgacga cttgaccctg ccaagcggca ccagatttct
    11881 tgcgtacgcg atcccctaag ccaaaggtgg cactcagggg aagcgcaaac tgccctgcaa
    11941 cgggagcgtt ggcttcatcg ctactttgac ccatgtcgaa tccttcttgt gaatctatta
    12001 tggcgacaag caaatcgagc tctgactgcc taccccacaa caactatcag aaagcaccag
    12061 cacaacggct gcctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
    12121 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
    12181 tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
    12241 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
    12301 gtttacgaac cgaacaggct tatgtcaact gggttcgtgc gagctcatcg atgcatttaa
    12361 tctttctgcg aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac
    12421 accaccgaaa aatagttact atcttcaaag ccacattcgg tcgaaatatc actgattaac
    12481 aggcggctat gctggagaag atattgcgca tgacacactc tgacctgtcg cagatattga
    12541 ttgatggtca ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat
    12601 gcctcatcac aaaatttatc cagcgcaaag ggacttttca ggctagccgc cagccgggta
    12661 atcagcttat ccagcaacgt ttcgctggat gttggcggca acgaatcact ggtgtaacga
    12721 tggcgattca gcaacatcac caactgcccg aacagcaact cagccatttc gttagcaaac
    12781 ggcacatgct gactactttc atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc
    12841 cccatgctac ctaagcgcca gtgtggttgc cctgcgctgg cgttaaatcc cggaatcgcc
    12901 ccctgccagt caagattcag cttcagacgc tccgggcaat aaataatatt ctgcaaaacc
    12961 agatcgttaa cggaagcgta ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca
    13021 cgggtaatgc gataagggcg atcgttgagt acatgcaggc cattaccgcg ccagacaatc
    13081 accagctcac aaaaatcatg tgtatgttca gcaaagacat cttgcggata acggtcagcc
    13141 acagcgactg cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc
    13201 gccaccgtgg ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat
    13261 acgttgagaa gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg
    13321 tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca
    13381 gcgataggcg atgtcagtaa cgctggcctc gctgtggcgt agcagatgtc gggctttcat
    13441 cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc gtttgctgct taagctgccg
    13501 atgtagcgta cgcagtgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc
    13561 ggcaaaatgg tcctccagcc aggccagaag caagttgaga cgtgatgcgc tgttttccag
    13621 gttctcctgc aaactgcttt tacgcagcaa gagcagtaat tgcataaaca agatctcgcg
    13681 actggcggtc gagggtaaat cattttcccc ttcctgctgt tccatctgtg caaccagctg
    13741 tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga gacggatact gcccatccag
    13801 ctcttgtggc agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata
    13861 cagcacattg gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg
    13921 tacgaaacag accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc
    13981 cgtgccatgt tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc
    14041 ctgcgggagc cggggttcta tcgccacgga cgcgttacca gacggaaaaa aatccacact
    14101 atgtaatacg gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag
    14161 gtcaggttct taccttaaat tttcgacgga aaaccacgta aaaaacgtcg atttttcaag
    14221 atacagcgtg aattttcagg aaatgcggtg agcatcacat
//
