LOCUS       sequence-45006-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(219-758)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             48..65
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
       61 gcagccatat ggattctgca aaaaaggctg cttgtgaatt ttctgagacg gatgtaacaa
      121 atactgaaca tcatcaaccc agtaataatg atttgaacac cactgagaag cgtgcagctg
      181 agaggcatcc agaaaagtat cagggtagtt ctgtttcaaa cttgcatgtg gagccatgtg
      241 gcacaaatac tcatgccagc tcattacagc atgagaacag cagtttatta ctcactaaag
      301 acagaatgaa tgtagaaaag gctgaattct gtaataaaag caaacagcct ggcttagcaa
      361 ggagccaaca taacagatgg gctggaagta aggaaacatg taatgatagg cggactccca
      421 gcacagaaaa aaaggtagat ctgaatgctg atcccctgtg tgagagaaaa gaatggaata
      481 agcagaaact gccatgctca gagaatccta gagatactga agatgttcct tggataacac
      541 taaatagcag cattcagaaa gttaatgagt ggttttccag aagtgatgaa ctgttaggtt
      601 ctgatgactc acatgatggg gagtctgaat caaatgccaa agtagctgat gtattggacg
      661 ttctaaatga ggtagatgaa tattctggtt cttcagagaa aatagactta ctggccagtg
      721 atcctcatga ggctttaata tgtaaaagtg aaagagttca ctccaaatca gtagagagta
      781 atattgaaga caaaatattt gggaaaacct atcggaagaa ggcaagcctc
//
