LOCUS       sequence-450061-       13459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses precursor peptide and lanthipeptide synthetase from 
            Streptomyces LanIV (FAST-RiPPs) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13459)
  AUTHORS   Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, 
            Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, 
            Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H
  TITLE     A scalable platform to discover antimicrobials of ribosomal origin.
  JOURNAL   Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w.
  PUBMED    36253467
REFERENCE   2  (bases 1 to 13459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-17"; volume: "13(1):6135. doi"; pages: " 
            10.1038/s41467-022-33890-w"
COMMENT     Sequence Label: pET28:LanIV-Streptomyces
FEATURES             Location/Qualifiers
     source          1..13459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        622..640
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    641..665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      3500..3547
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3588..4043
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3675..3694)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3885..3906
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4135..4950)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4135..4950)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4858..4877
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4942..4961
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5072..5660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5814..5831
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5846..5985
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5911..5930)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6071..6093
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6087..6278)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7087..8169)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8131..8150
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8170..8247)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     8453..8472
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        8560..8578
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8579..8603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8620..8637
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      8948..8995
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        9000..9018
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9019..9043
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9060..9077
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      9364..9411
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        9416..9434
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9435..9459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      12294..12341
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        12365..12428
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12429..12497,12498..13289)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12647..12666)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggaattc ggtctcagct ctaatacgac tcactatagg ggaattgtga gcggataaca
      661 attccaagga gatatacaat gtcagcatta ccagaaactg attcaccgtc cggccctggt
      721 cctcgtcata gtcgtgctga acgtgccgcc gatcttgcgc tgttaccaga tttggtccgt
      781 gcagttattg cccgtcaggc agcgggtgcc gaacaagaat ggtctgtcga tcagggcgaa
      841 ttttggtgcc aagttgtacc accaggtgct gtgcgtcgtg cccagggatg gaaactgcat
      901 gtatctgcaa ccccgcttgc ggcgccttta acccttgccc gtgcggccga agtattagtt
      961 gcccatcgtt gtcgttttaa atttgcggga agccctgccc gtgttgcggg actggttagt
     1021 ggtcgttttg cacgcggcgg ttcgggtaaa tttattacag tgtatcctgc tgatgatgaa
     1081 caatttcgtt tgcttgcgga agaattacat cgcgcaactg caggtttacc gggtcctgcg
     1141 gttcttagcg atcgtcgtta tcgtcaagat tcgcaagtat tttaccgtta cggagtcttt
     1201 agcgcagtcc gttcacttac gccagacggc ggctacgcgg cactgttaac cgatccggaa
     1261 ggtcgtcctg ttgcagacga gcgtaatgcc tggttctcac cgccggcttg ggccggagat
     1321 ccgtttcctg accgtccggc agcaccggtg cgttctaccg ccacagctaa accggtcttg
     1381 ttgaatggtc gttatctggt gcgtggagcg attcaacatt cgaataaagg tggagtattc
     1441 cgcgcagaag atagtcgtac gggtgaacaa gtcgttgtaa aacaagctcg tgcacatgtg
     1501 ggcgcaggct tagaaggctt ggatgtacgt gatcttttac gtcgtgaagc agcattactg
     1561 gagcgtctgg gtggagatcg tccagatcgt gtcgtacctc gtgttgttga agtgtttgag
     1621 caacaaggta gcgtgttcat ggcggtagaa tctgttcctg gtgcaacact gcgtcgtacc
     1681 gtagcacgtc gcttggcaga agatggcacg gcatgtccgg atgcaggtac tgcaggttct
     1741 ctcgtaggcc aactggttga actggttgcg gcggctcatg aacctgggtt aaccttacat
     1801 gactttaacc caaataacgt tatggtcgct cctgatggtc gccttttatt gattgactta
     1861 gaaatggcag ctcctcaagg tgaacgctgg gttcttggtg ccaccccggg ttacactgct
     1921 cccgaattgc gtgcagctga tcacgtagcg ccagcattgc cgccctcggt tgatttgttt
     1981 gcgttaggtg ctacggtttt acatgcattg agcggggcgg acccattgtt tgccccggat
     2041 ttgccggaag ctgcagcacg tcctgatgaa gaacgtctgg cggctttagt cgaacttctt
     2101 agtcgtgatc aaccgttggt gcgcgactat gccccacttg tccttggtct gatgcgtgat
     2161 gaacctgctg aacgttggac cctggaagct gcgcgtacgt ttcgtccggc agcagcaggt
     2221 aacgatcgca ctggaacgac ggatcgtgcc gatcgtgcag gtgatactcg tgatcgtctg
     2281 ttacatgatg gtattgcaca tttacttgcg accatgcgtc ctggagatcc cgagcgcctt
     2341 tggccgagcg acagctttgg tgaaacctgt gatccggtta ccttgcaaca tggtagcgcg
     2401 ggtggagtag cagttctggc ccgtgcctta cgtcaatggg atgatcgtcc tgcggaagca
     2461 cctgcgcccg tttcaggtga agctctgcgc gaaggtttgc gtgccgccgc tcactggacc
     2521 gccgaccgtc aagatgaact gccaggcgat ttaccgggtt tatattttgg tcgttcgggt
     2581 actgcttggg cgctgttgga tgcggcacgt gcgttggatg ataccgattt agcagaacgt
     2641 gcaaccaagc ttgctctgga attgcctgta cgttggccaa accccgatgt tacgcatgga
     2701 gctgcgggtt cgggtctggc acaattacat ttctggcatg gaacaggtga tgatcgtttc
     2761 ttagatcgtg ctgcacaagc cgcagaaggc ttactggccg ctgcacgtca cacccctgag
     2821 ggtgtgttct ggccagttcc agaggacttt ggaagtgcat tagctggtgc atggcattat
     2881 ggttttgcac acggcgtcgc tggcgtgggt acattcttgt tattggcggc tggagctaca
     2941 ggtgatgaac gttttcgtgc cgcagccgtt gcagcgggcg aaactctgac caccgccgcg
     3001 cgttcgggcc cggcaggcac cttgtggcct gttgatcgtg cacgtcatct ttctgacagt
     3061 ccagatctgg cccgtcattg gtgttctgga agctcaggag ttgggacgtt tctggtccgt
     3121 ctctgggcgg ctggcggtgc aggtgacgat accttacgtg ctttagttga aggtgcagca
     3181 gatgccgttc gcgcgggtcg tgtaaccgac tcacctgcga cttgtcacgg tcttgcagga
     3241 aatgctcaac tgttattaga tgtagcagaa ttgacgggag atgatcgtca tcgtgcagat
     3301 gctgaattgt ttgtagaaca tttggcggcc caagcagtgc tgcgtgatgg acgtctgctg
     3361 gtacctgatg aaaatcgtcg taccgtgctg gctgaatacg cgaccggttt ggctggaagt
     3421 ctgagcttat tattgcgtct gcgctacggt gggccacgcg cgtggttacc tgaaggcgca
     3481 cgtaccacac cttgacggtc tagcataacc ccttggggcc tctaaacggg tcttgagggg
     3541 ttttttgagc gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     3601 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3661 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3721 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3781 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3841 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3901 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3961 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4021 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     4081 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa
     4141 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     4201 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     4261 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     4321 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     4381 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     4441 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     4501 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     4561 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     4621 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     4681 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     4741 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     4801 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     4861 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     4921 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     4981 agacagtttt attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5041 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5101 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5161 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5221 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5281 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5341 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5401 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5461 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5521 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5581 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5641 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5701 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5761 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5821 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     5881 gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5941 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     6001 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6061 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6121 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     6181 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     6241 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     6301 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     6361 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     6421 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     6481 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     6541 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     6601 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     6661 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     6721 aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg
     6781 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
     6841 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     6901 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     6961 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     7021 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     7081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7141 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     7201 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     7261 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     7321 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac
     7381 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     7441 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     7501 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     7561 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     7621 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     7681 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     7741 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     7801 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     7861 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     7921 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     7981 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     8041 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     8101 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     8161 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
     8221 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
     8281 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     8341 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     8401 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     8461 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     8521 cggcgtagag gatcgagatc tcgatcccgc gaaatatggt aatacgactc actatagggg
     8581 aattgtgagc ggataacaat tccaaggaga tatacaatgc accaccacca ccaccacgca
     8641 atggccgccg ggcatcgtca accgtttcgt accccagatc gtcatcccag ccgcccaaaa
     8701 gaaactgaaa tgatggagca tgatatcgat gcgttacagg tactgccgga agatgtgcgc
     8761 acggaagatg cacgcggtga agaacatgcg gcagcgttaa ttacggcatg tcgttggcat
     8821 accagcgggt gttgaggctc cttcatctga caacgtgcaa ccgctatcgc catcgattga
     8881 ttcagcggac ggtgttgtcg tcatagtttg ggcatgtttc ccttgtaggt gtgaaatcac
     8941 taacggtcta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgccctt
     9001 aatacgactc actatagggg aattgtgagc ggataacaat tccaaggaga tatacaatgc
     9061 atcatcatca tcaccatgca atgcaccgtc gcggtccagc ccgttgtcgt agtcccgcag
     9121 taagttatcc cggaactggc cataatgatg gagatcctgc acgttcatcg gatcgtactg
     9181 gttccccttg tgttcgttgg acctgtggcg atctgaatcc tcgtccgcct cgctgtgaac
     9241 gtggcgcgtt gccagattgt gcaacgggtc cgtgtaacga ataaggctcc ttcatctgac
     9301 aacgtgcaac cgctatcgcc atcgattgat tcagcggacg gtgttgtcgt catagtccgc
     9361 ggtctagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt ggctctaata
     9421 cgactcacta taggggaatt gtgagcggat aacaattcca aggagatata caatgtcagc
     9481 attaccagaa actgattcac cgtccggccc tggtcctcgt catagtcgtg ctgaacgtgc
     9541 cgccgatctt gcgctgttac cagatttggt ccgtgcagtt attgcccgtc aggcagcggg
     9601 tgccgaacaa gaatggtctg tcgatcaggg cgaattttgg tgccaagttg taccaccagg
     9661 tgctgtgcgt cgtgcccagg gatggaaact gcatgtatct gcaaccccgc ttgcggcgcc
     9721 tttaaccctt gcccgtgcgg ccgaagtatt agttgcccat cgttgtcgtt ttaaatttgc
     9781 gggaagccct gcccgtgttg cgggactggt tagtggtcgt tttgcacgcg gcggttcggg
     9841 taaatttatt acagtgtatc ctgctgatga tgaacaattt cgtttgcttg cggaagaatt
     9901 acatcgcgca actgcaggtt taccgggtcc tgcggttctt agcgatcgtc gttatcgtca
     9961 agattcgcaa gtattttacc gttacggagt ctttagcgca gtccgttcac ttacgccaga
    10021 cggcggctac gcggcactgt taaccgatcc ggaaggtcgt cctgttgcag acgagcgtaa
    10081 tgcctggttc tcaccgccgg cttgggccgg agatccgttt cctgaccgtc cggcagcacc
    10141 ggtgcgttct accgccacag ctaaaccggt cttgttgaat ggtcgttatc tggtgcgtgg
    10201 agcgattcaa cattcgaata aaggtggagt attccgcgca gaagatagtc gtacgggtga
    10261 acaagtcgtt gtaaaacaag ctcgtgcaca tgtgggcgca ggcttagaag gcttggatgt
    10321 acgtgatctt ttacgtcgtg aagcagcatt actggagcgt ctgggtggag atcgtccaga
    10381 tcgtgtcgta cctcgtgttg ttgaagtgtt tgagcaacaa ggtagcgtgt tcatggcggt
    10441 agaatctgtt cctggtgcaa cactgcgtcg taccgtagca cgtcgcttgg cagaagatgg
    10501 cacggcatgt ccggatgcag gtactgcagg ttctctcgta ggccaactgg ttgaactggt
    10561 tgcggcggct catgaacctg ggttaacctt acatgacttt aacccaaata acgttatggt
    10621 cgctcctgat ggtcgccttt tattgattga cttagaaatg gcagctcctc aaggtgaacg
    10681 ctgggttctt ggtgccaccc cgggttacac tgctcccgaa ttgcgtgcag ctgatcacgt
    10741 agcgccagca ttgccgccct cggttgattt gtttgcgtta ggtgctacgg ttttacatgc
    10801 attgagcggg gcggacccat tgtttgcccc ggatttgccg gaagctgcag cacgtcctga
    10861 tgaagaacgt ctggcggctt tagtcgaact tcttagtcgt gatcaaccgt tggtgcgcga
    10921 ctatgcccca cttgtccttg gtctgatgcg tgatgaacct gctgaacgtt ggaccctgga
    10981 agctgcgcgt acgtttcgtc cggcagcagc aggtaacgat cgcactggaa cgacggatcg
    11041 tgccgatcgt gcaggtgata ctcgtgatcg tctgttacat gatggtattg cacatttact
    11101 tgcgaccatg cgtcctggag atcccgagcg cctttggccg agcgacagct ttggtgaaac
    11161 ctgtgatccg gttaccttgc aacatggtag cgcgggtgga gtagcagttc tggcccgtgc
    11221 cttacgtcaa tgggatgatc gtcctgcgga agcacctgcg cccgtttcag gtgaagctct
    11281 gcgcgaaggt ttgcgtgccg ccgctcactg gaccgccgac cgtcaagatg aactgccagg
    11341 cgatttaccg ggtttatatt ttggtcgttc gggtactgct tgggcgctgt tggatgcggc
    11401 acgtgcgttg gatgataccg atttagcaga acgtgcaacc aagcttgctc tggaattgcc
    11461 tgtacgttgg ccaaaccccg atgttacgca tggagctgcg ggttcgggtc tggcacaatt
    11521 acatttctgg catggaacag gtgatgatcg tttcttagat cgtgctgcac aagccgcaga
    11581 aggcttactg gccgctgcac gtcacacccc tgagggtgtg ttctggccag ttccagagga
    11641 ctttggaagt gcattagctg gtgcatggca ttatggtttt gcacacggcg tcgctggcgt
    11701 gggtacattc ttgttattgg cggctggagc tacaggtgat gaacgttttc gtgccgcagc
    11761 cgttgcagcg ggcgaaactc tgaccaccgc cgcgcgttcg ggcccggcag gcaccttgtg
    11821 gcctgttgat cgtgcacgtc atctttctga cagtccagat ctggcccgtc attggtgttc
    11881 tggaagctca ggagttggga cgtttctggt ccgtctctgg gcggctggcg gtgcaggtga
    11941 cgatacctta cgtgctttag ttgaaggtgc agcagatgcc gttcgcgcgg gtcgtgtaac
    12001 cgactcacct gcgacttgtc acggtcttgc aggaaatgct caactgttat tagatgtagc
    12061 agaattgacg ggagatgatc gtcatcgtgc agatgctgaa ttgtttgtag aacatttggc
    12121 ggcccaagca gtgctgcgtg atggacgtct gctggtacct gatgaaaatc gtcgtaccgt
    12181 gctggctgaa tacgcgaccg gtttggctgg aagtctgagc ttattattgc gtctgcgcta
    12241 cggtgggcca cgcgcgtggt tacctgaagg cgcacgtacc acaccttgac ggtctagcat
    12301 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gagcgtgaga ccccatggtt
    12361 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    12421 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    12481 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    12541 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    12601 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    12661 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    12721 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    12781 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    12841 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12901 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    12961 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    13021 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    13081 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    13141 gtggctcccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    13201 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    13261 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    13321 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    13381 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    13441 aaaagatcaa aggatcttc
//
