LOCUS       sequence-450065-        9714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone for lentiviral expression of AID degron tagged POI 
            and mCherry fused BlasticidinR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, 
            Wan J, Fang D, Bintu L
  TITLE     The H3.3 K36M oncohistone disrupts the establishment of epigenetic 
            memory through loss of DNA methylation.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147.
  PUBMED    37873347
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147."
COMMENT     Sequence Label: pLenti-MCS-3AID-HA-2A-mCherryBSD
FEATURES             Location/Qualifiers
     source          1..9714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2057..2455)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(2474..3181)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2749..2768)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3018..3036
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(3182..3235)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3245..3271)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3284..3310)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3311..3514)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3521..3724)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3731..3934)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3959..3985)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3986..4189)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4196..4399)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4406..4609)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4634..4660)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4717..5655
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4740..4760)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(5656..5867)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    5990..6107
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6405..6449)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6634..6867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7364..7489
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7536..7716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7734..7932)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7759..7779)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7934..8313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8485..8504
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8579..8683
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8684..8752,8753..9544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8902..8921)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc
     2101 caactgccgt cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat
     2161 cgagaagcac ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga
     2221 tcgcgacgat acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga
     2281 gttctgcaca aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt
     2341 cgctagagag agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg
     2401 tagccgttgc tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatgctac
     2461 cgctgccgct acccttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
     2521 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
     2581 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
     2641 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
     2701 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
     2761 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
     2821 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
     2881 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
     2941 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
     3001 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
     3061 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
     3121 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
     3181 ttgggccagg attctcctcg acatctccgc aagttaggag gcttcccctt ccctctccag
     3241 atccagcata atcaggcaca tcataagggt atccggcgct gccagcgtag tcaggcacat
     3301 cataggggta tttatacatt cgcagatcaa ttttccgcaa gtagggggct ccgtccatac
     3361 ttacttttac aaatgctgcc gcctcggggc ctccagaact cttttggcaa ctgaccatta
     3421 cattcttgcg gtatgatctg acggggggcc aaccgactac ttgagctttt gccgggggtt
     3481 tagctgggtc tttcggacat gcactcttct ctttggaaga tttatacatg cggagatcta
     3541 ttttcctaag gtacggggcg ccgtccatgc taactttaac gaatgctgca gcctctggtc
     3601 cgccactaga cttttgacaa gataccataa cattcttgcg ataggatctg acaggcggcc
     3661 aaccaactac ttgagctttg gcgggtggct tcgcagggtc cttcgggcag gcgctctttt
     3721 ctttgctgct cttatacatc ctaaggtcga ttttcctcaa gtacggcgca ccatccatgg
     3781 aaacctttac aaacgctgcg gcctctggac ctccgcttga tttttgacat gataccataa
     3841 catttttcct gtatgagcgc acaggaggcc aaccgacgac ctgagccttc gccggcggct
     3901 ttgcagggtc ttttggacag gcacttttct ccttactcaa ttggccggaa atcgatggtg
     3961 cataatcagg aacatcatag gggtatttat acattcgcag atcaattttc cgcaagtagg
     4021 gggctccgtc catacttact tttacaaatg ctgccgcctc ggggcctcca gaactctttt
     4081 ggcaactgac cattacattc ttgcggtatg atctgacggg gggccaaccg actacttgag
     4141 cttttgccgg gggtttagct gggtctttcg gacatgcact cttctctttg gaagatttat
     4201 acatgcggag atctattttc ctaaggtacg gggcgccgtc catgctaact ttaacgaatg
     4261 ctgcagcctc tggtccgcca ctagactttt gacaagatac cataacattc ttgcgatagg
     4321 atctgacagg cggccaacca actacttgag ctttggcggg tggcttcgca gggtccttcg
     4381 ggcaggcgct cttttctttg ctgctcttat acatcctaag gtcgattttc ctcaagtacg
     4441 gcgcaccatc catggaaacc tttacaaacg ctgcggcctc tggacctccg cttgattttt
     4501 gacatgatac cataacattt ttcctgtatg agcgcacagg aggccaaccg acgacctgag
     4561 ccttcgccgg cggctttgca gggtcttttg gacaggcact tttctcctta ctcaattggc
     4621 cggaaatcga tggtgcataa tcaggaacat cataaggata gccaccgtta acagagacgc
     4681 ttgacccgtc tccatttaaa tgctagctca cgacacctga aatggaagaa aaaaactttg
     4741 aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa
     4801 ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg
     4861 aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagcgcga
     4921 gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc
     4981 tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg
     5041 ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc tttccgctca
     5101 cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc
     5161 gaggccaggc accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg
     5221 ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc
     5281 gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact
     5341 atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt
     5401 atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg
     5461 cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga
     5521 actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt
     5581 ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc
     5641 acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc
     5701 gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac
     5761 gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac
     5821 ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcaca
     5881 tgcatggcgg taatacggtt atccacgcgg ccgcctgagt caaatgggat ctctagagcg
     5941 ggcccggtac taaccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa
     6001 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     6061 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
     6121 ctgccatttg tctgcagaat tggcgcacgc agtgccgatc cgttcactaa tcgaatggat
     6181 ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc
     6241 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
     6301 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
     6361 aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta
     6421 tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag
     6481 gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga
     6541 ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg
     6601 caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt
     6661 tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga
     6721 tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat
     6781 aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct
     6841 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
     6901 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
     6961 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
     7021 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
     7081 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
     7141 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
     7201 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
     7261 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
     7321 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
     7381 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
     7441 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     7501 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     7561 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     7621 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     7681 ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaac gcgctgctta
     7741 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     7801 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     7861 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     7921 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     7981 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     8041 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     8101 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     8161 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     8221 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     8281 attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc
     8341 gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt
     8401 gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag
     8461 ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     8521 ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg
     8581 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8641 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8701 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8761 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8821 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8881 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8941 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9001 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9061 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9121 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9181 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9241 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9301 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9361 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9421 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9481 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9541 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9601 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9661 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
