LOCUS       sequence-450067-        9723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone for lentiviral expression of AID degron tagged POI 
            and mTurquoise fused BlasticidinR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, 
            Wan J, Fang D, Bintu L
  TITLE     The H3.3 K36M oncohistone disrupts the establishment of epigenetic 
            memory through loss of DNA methylation.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147.
  PUBMED    37873347
REFERENCE   2  (bases 1 to 9723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147."
COMMENT     Sequence Label: pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
FEATURES             Location/Qualifiers
     source          1..9723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2057..2455)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(join(2474..3184,3185..3187,3188..3190))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2516..2537)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2865..2884
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3124..3145
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3191..3244)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3254..3280)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3293..3319)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3320..3523)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3530..3733)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3740..3943)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3968..3994)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3995..4198)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4205..4408)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4415..4618)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4643..4669)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4726..5664
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4749..4769)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(5665..5876)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    5999..6116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6414..6458)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6643..6876
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7373..7498
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7545..7725
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7743..7941)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7768..7788)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7943..8322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8494..8513
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8588..8692
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8693..8761,8762..9553)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8911..8930)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc
     2101 caactgccgt cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat
     2161 cgagaagcac ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga
     2221 tcgcgacgat acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga
     2281 gttctgcaca aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt
     2341 cgctagagag agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg
     2401 tagccgttgc tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatgctac
     2461 cgctgccgct acccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     2521 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     2581 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     2641 ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg
     2701 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtcgctg atgtagttgt
     2761 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     2821 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     2881 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     2941 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggacaggg
     3001 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     3061 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     3121 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     3181 tgctcaccat tgggccagga ttctcctcga catctccgca agttaggagg cttccccttc
     3241 cctctccaga tccagcataa tcaggcacat cataagggta tccggcgctg ccagcgtagt
     3301 caggcacatc ataggggtat ttatacattc gcagatcaat tttccgcaag tagggggctc
     3361 cgtccatact tacttttaca aatgctgccg cctcggggcc tccagaactc ttttggcaac
     3421 tgaccattac attcttgcgg tatgatctga cggggggcca accgactact tgagcttttg
     3481 ccgggggttt agctgggtct ttcggacatg cactcttctc tttggaagat ttatacatgc
     3541 ggagatctat tttcctaagg tacggggcgc cgtccatgct aactttaacg aatgctgcag
     3601 cctctggtcc gccactagac ttttgacaag ataccataac attcttgcga taggatctga
     3661 caggcggcca accaactact tgagctttgg cgggtggctt cgcagggtcc ttcgggcagg
     3721 cgctcttttc tttgctgctc ttatacatcc taaggtcgat tttcctcaag tacggcgcac
     3781 catccatgga aacctttaca aacgctgcgg cctctggacc tccgcttgat ttttgacatg
     3841 ataccataac atttttcctg tatgagcgca caggaggcca accgacgacc tgagccttcg
     3901 ccggcggctt tgcagggtct tttggacagg cacttttctc cttactcaat tggccggaaa
     3961 tcgatggtgc ataatcaggc acatcatagg ggtatttata cattcgcaga tcaattttcc
     4021 gcaagtaggg ggctccgtcc atacttactt ttacaaatgc tgccgcctcg gggcctccag
     4081 aactcttttg gcaactgacc attacattct tgcggtatga tctgacgggg ggccaaccga
     4141 ctacttgagc ttttgccggg ggtttagctg ggtctttcgg acatgcactc ttctctttgg
     4201 aagatttata catgcggaga tctattttcc taaggtacgg ggcgccgtcc atgctaactt
     4261 taacgaatgc tgcagcctct ggtccgccac tagacttttg acaagatacc ataacattct
     4321 tgcgatagga tctgacaggc ggccaaccaa ctacttgagc tttggcgggt ggcttcgcag
     4381 ggtccttcgg gcaggcgctc ttttctttgc tgctcttata catcctaagg tcgattttcc
     4441 tcaagtacgg cgcaccatcc atggaaacct ttacaaacgc tgcggcctct ggacctccgc
     4501 ttgatttttg acatgatacc ataacatttt tcctgtatga gcgcacagga ggccaaccga
     4561 cgacctgagc cttcgccggc ggctttgcag ggtcttttgg acaggcactt ttctccttac
     4621 tcaattggcc ggaaatcgat ggtgcataat caggaacatc ataaggatag ccaccgttaa
     4681 cagagacgct tgacccgtct ccatttaaat gctagctcac gacacctgaa atggaagaaa
     4741 aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc
     4801 aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc
     4861 agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca
     4921 aaagcgcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga
     4981 agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc
     5041 tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa gcggccatct
     5101 ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca
     5161 cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc cccgtccgat
     5221 tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg acgcacgggc
     5281 cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt ggcccgcatt
     5341 tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc
     5401 tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc gcacgcggcg
     5461 gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc cttaagcgca
     5521 aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa tcacgtactg
     5581 cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga ggccaggccc
     5641 gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc gaaaaagaac
     5701 gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag
     5761 ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca
     5821 attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg
     5881 acgggcacat gcatggcggt aatacggtta tccacgcggc cgcctgagtc aaatgggatc
     5941 tctagagcgg gcccggtact aaccaaactg gatctctgct gtccctgtaa taaacccgaa
     6001 aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta
     6061 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt
     6121 ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat
     6181 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
     6241 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     6301 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     6361 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     6421 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     6481 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     6541 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     6601 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     6661 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     6721 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     6781 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     6841 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     6901 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     6961 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
     7021 atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     7081 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     7141 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     7201 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     7261 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     7321 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     7381 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     7441 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     7501 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     7561 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     7621 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     7681 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaacg
     7741 cgctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     7801 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     7861 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     7921 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     7981 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     8041 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     8101 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     8161 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     8221 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     8281 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc
     8341 gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg
     8401 tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca
     8461 cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag
     8521 tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     8581 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8641 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8701 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8761 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8821 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8881 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8941 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9001 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9061 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9121 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9181 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9241 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9301 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9361 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9421 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9481 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9541 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9601 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9661 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9721 ttc
//
