LOCUS       sequence-450073-        8817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-to-T base editing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Liu Z, Chen S, Davis AE, Lo CH, Wang Q, Li T, Ning K, Zhang Q, Zhao 
            J, Wang S, Sun Y
  TITLE     Efficient Rescue of Retinal Degeneration in Pde6a Mice by Engineered
            Base Editing and Prime Editing.
  JOURNAL   Adv Sci (Weinh). 2024 Sep 19:e2405628. doi: 10.1002/advs.202405628.
  PUBMED    39297417
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv Sci 
            (Weinh). 2024 Sep 19:e2405628. doi: 10.1002/advs.202405628."
COMMENT     Sequence Label: AID-N51A-NG-CBE
FEATURES             Location/Qualifiers
     source          1..8817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1351..5451
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5482..5730
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5761..6009
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6052..6072
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6081..6098
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6121..6138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6127..6351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6422..6438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6422..6438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(join(6470..6476,6477..6494,6495..6500))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6515..6536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6653..6670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6824..7412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6904..6923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7583..8374,8375..8443))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8206..8225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8444..8548)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8627..8646)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcagcaaaga agaagcggaa agtcgatagc
      721 ctgctgatga accgtcgtga atttctgtat cagtttaaaa acgtgcgttg ggcgaaaggc
      781 cgtcgtgaaa cctatctgtg ctatgtggtg aaacgtcgtg atagcgcgac cagctttagc
      841 ctggattttg gctatctgcg tgccaaaaac ggctgccatg tggaactgct gtttctgcgt
      901 tatattagcg attgggatct ggatccgggc cgttgctatc gtgtgacctg gtttatcagc
      961 tggagcccgt gctatgattg cgcgcgtcat gtggcggatt ttctgcgtgg caacccgaac
     1021 ctgagcctgc gtatttttac cgcgcgtctg tatttttgcg aagccggcag gcgtgaaccg
     1081 gaaggcctgc gtcgtctgca tcgtgcgggc gtgcagattg cgattatgac ctttaaagat
     1141 tatttttatt gctggaacac ctttgtggaa aaccatggac gtacctttaa agcgtgggaa
     1201 ggcctgcatg aaaacagcgt gcgtctgagc cgtcagctgc gtcgtattct gctgtctggc
     1261 ggatctagcg gaggatcctc tggcagcgag acaccaggaa caagcgagtc agcaacacca
     1321 gagagcagtg gcggcagcag cggcggcagc gacaagaagt acagcatcgg cctggccatc
     1381 ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa
     1441 ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg
     1501 ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga
     1561 tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc
     1621 aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag
     1681 aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag
     1741 taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg
     1801 cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag
     1861 ggcgacctga accccgacaa cagcgacgtg gacaagctat tcatccagct ggtgcagacc
     1921 tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc
     1981 ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc
     2041 gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac
     2101 ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac
     2161 gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg
     2221 gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag
     2281 atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac
     2341 ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc
     2401 ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag
     2461 ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg
     2521 aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc
     2581 caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca
     2641 ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac
     2701 gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa
     2761 accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc
     2821 atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac
     2881 agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc
     2941 gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg
     3001 ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa
     3061 atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg
     3121 ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa
     3181 aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg
     3241 atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg
     3301 aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg
     3361 gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga
     3421 aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc
     3481 caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc
     3541 gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg
     3601 ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag
     3661 aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg
     3721 ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg
     3781 tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac
     3841 cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa ggacgactcc
     3901 atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc
     3961 tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg
     4021 attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg
     4081 gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg
     4141 gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg
     4201 gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag
     4261 ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc
     4321 gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc
     4381 gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag
     4441 gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc
     4501 ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag
     4561 atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa
     4621 gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatccgg
     4681 cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac
     4741 ggcggcttcg tgagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag
     4801 ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga
     4861 agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa
     4921 aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag
     4981 agaatgctgg cctctgcccg gttcctgcag aagggaaacg aactggccct gccctccaaa
     5041 tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat
     5101 aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag
     5161 cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg
     5221 tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac
     5281 ctgtttaccc tgaccaatct gggagcccct cgggccttca agtactttga caccaccatc
     5341 gaccggaagg tgtaccggag caccaaagag gtgctggacg ccaccctgat ccaccagagc
     5401 atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggtga cagcggcggg
     5461 agcggcggga gcggggggag cactaatctg agcgacatca ttgagaagga gactgggaaa
     5521 cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga agtgatcggc
     5581 aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac agatgagaat
     5641 gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt catccaggat
     5701 tctaacggcg agaataagat caagatgctg agcggaggat ccggaggatc tggaggcagc
     5761 accaacctgt ctgacatcat cgagaaggag acaggcaagc agctggtcat ccaggagagc
     5821 atcctgatgc tgcccgaaga agtcgaagaa gtgatcggaa acaagcctga gagcgatatc
     5881 ctggtccata ccgcctacga cgagagtacc gacgaaaatg tgatgctgct gacatccgac
     5941 gccccagagt ataagccctg ggctctggtc atccaggatt ccaacggaga gaacaaaatc
     6001 aaaatgctgt ctggcggctc aaaaagaacc gccgacggca gcgaattcga gcccaagaag
     6061 aagaggaaag tctaaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
     6121 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6181 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6241 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6301 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6361 cggaaagaac cagctggggc tcgataccgt cgacctctag ctagagcttg gcgtaatcat
     6421 ggtcatagct gtttcctgtg tgaaattatt atccgctcac aattccacac aacatacgag
     6481 ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt gagctaactc acattaattg
     6541 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6601 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg cacttccgct tcctcgctca
     6661 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6721 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6781 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6841 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6901 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6961 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7021 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7081 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7141 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7201 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7261 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7321 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7381 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7441 ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7501 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7561 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7621 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7681 gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca cgctcaccgg
     7741 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7801 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7861 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7921 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7981 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8041 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8101 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8161 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8221 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8281 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8341 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8401 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8461 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8521 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8581 acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca atctgctctg
     8641 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     8701 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     8761 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
