LOCUS       sequence-450091-       10183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible zebrafish gfap promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 10183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p5E-gfap
FEATURES             Location/Qualifiers
     source          1..10183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..47
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(206..233)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(325..411)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     460..482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     474..491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     475..491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    531..626
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        628..646
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8095..8111)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8095..8111)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8148..8166)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    8167..8290
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(8363..8381)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8386..8402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8386..8402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8504..8523)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8515..9324
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8582..8601)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      9471..10059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9960..9979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
       61 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      121 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
      181 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
      241 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
      301 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
      361 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
      421 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
      481 cgacggccag tcttaagctc gggcccgagt taacgctacc atggagctcc aaataatgat
      541 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gcttttttat
      601 aatgccaact ttgtatagaa aagttggtaa tacgactcac tatagggcga attgggtacc
      661 gggccccccc tcgaggtaag gactgaggtg atgcacagtg ttgggtatgt tactttcaat
      721 aagttattct ttgctaatta ttagtcattt gaattgtatt gaaattacat ttttagttac
      781 tttgcctaaa aaaaaacttg ataagtaatc gaattacata cttgaagtcc acataaatct
      841 cattcattca ttccttctct tttcgactta gtccctttat taatcagggg tttctacagc
      901 ggaacgaacc tcatatgttt caatttagtg actatttttt ataaattatt gacaatgttt
      961 ttgttgcttg ttcaaactac ttatttaaaa tgagctgaaa ctgcacaatt attgatattt
     1021 cattggaaca gcttaatttt ttttatgttt aatccactta aataaaattc caattatata
     1081 atattccaat tataaagtta cttaatcaat ttgtattagg acaatatgaa tgaattgtgt
     1141 ggaaccctgc attttttaca gtgaaccttt ccttgtttct taaacatggg tgattttcgg
     1201 agaaataaaa tttgacaata tctgaaatgt aaaaagaaaa tattcagtta ctctgccttt
     1261 ctaaatgcat agtttatatt atcatcagag ttgtaaagta actagttaca aatactcaaa
     1321 ttactgtaat tgagtagttt ttttcaggaa ttgtaattta ctaagtagct aaaaaatgtg
     1381 tacttttact ttcccttgag tacattatta atgctgtaac ggtactttta ctccactact
     1441 ttccttcagc ctgcactcac tactttgttt tatcttgtct atgaggatta gaaaaatcaa
     1501 tcctgtgatt ccaatcaaat cgcacatcca aagtctttac aaaagcattt tgtttggatg
     1561 tgaaaatcgg gttgatgtca gaactcaatg tcagcccaac gtcaatgtcc aacctaaaat
     1621 caacctaata tcaacgtcta atgatgttac agcttgacgt tgtgtgggcg ttaccattat
     1681 ttgtcagaca ttggatttta gttgccatac ctgataaata aatgtcagta tttgatgtcc
     1741 gtatgatctt ggatttagat gttggctcga cattggattt tggtcacctg acataacaac
     1801 ctaaatctat cctaataata acgtcttatg acgttgtgtg ccaaccattt ttgtttatta
     1861 aaaatttata taaacattta caataatata ttatccatgt gatttaatag aaatgttatt
     1921 tgtttttttt ctcccccata tttctgaatg acttaaagct tttattatca ggataaggtt
     1981 gtggttttta ttttttgctt gctaacttca attgctattg catttacatt tatgcaaatg
     2041 gggagacgcg gtgggtcagt tggcgtttct gtgtggagtt tgcatgttct ccccgtgttc
     2101 gcgtgggttt tctctgggtg ctctggtttc ccctaaagac atgtagtata ggtgaattgg
     2161 gtaagctaaa ttgtccgtag tgtgtatgtg tgaatgtatg gatgttttcc tgtgatgggt
     2221 tgcagctgaa agggcatccg ctgtgtaaaa catatgctgg ataagttggc aattcatttc
     2281 gctgtggtaa ccccagatta atatcggaac tacgccaaaa agaaaatgaa tgaatgagtg
     2341 aattaatgca aatggctcat ccttccacct aattccagtg ccttgcattg cattcaagct
     2401 ctacaattaa tcagttcctt tattttcact ttccaaactg aatccaacaa ctcatgagga
     2461 ccccagggtg ccttgattta ccagtgtcat gatgaacaca atctacccca ctttaaccca
     2521 ctcgtcaaca taaatactcc cttaaagggt gtacttgtgt ctgagtagaa gtgtctgctg
     2581 ggttggaggt gtcgcacatt ccaggacacg tcaagaagaa cgctaagagc aacaggagaa
     2641 caatgtcaca aaaaaggtca aagaaacgcc atccattagc cagctttata aacagctgtc
     2701 tcgtggctaa agaagttctc aagaataacc aaccttgcat ttttccacat tagttttaca
     2761 ccgcagcaaa gtcaccatgt ggatttatag catccaaacc ccattttcaa tgagaggctt
     2821 cacatttctc gctctcattc aatcccacac tgcctctctt ttcagactaa atcctgatag
     2881 gatgagtggt atgggggcgg ggagggggca caatacctca gtccagcttc cacaaagact
     2941 caacacaccc atgcagatgt cctgttgttg gggaagtgca tttgtaacca aatttcaaac
     3001 tttcaacttt catttgaagc agtggagact gctccattcc tattcaagat caatttattg
     3061 aaatatgaca acatttagaa taactttgtt atatgttatt atgttttata atgcaatttt
     3121 gtttctgtga catacatttt tttatttagt gcagtcttca gagccacagg aaccttgaga
     3181 atcattctaa ttatgatttg ctactcgaaa aacatttttc atcatcaata ataagaaatt
     3241 aaaacggtat acatatagat gtttagtata acagttctta gtagtttaaa agaacatttg
     3301 taacaacatt ccaacctttt aataacttta gccttgctta catttctaaa ggagagcctt
     3361 ccagctgttt ttctgaaccc agtgatgaat gtaaagttct taaaaccaag ttctaagaat
     3421 gttttaatga ttacatttaa cccaatttca agccaaattt cctataccac gagtaaaata
     3481 tagttataat taggatagat ctttggtaaa ataagataca attttaagat ttgaatttaa
     3541 aagtagctaa attgtttttt tatgacagaa cttttaagtt tatagaatat taatacagga
     3601 caaaacttgt aaatgtattt aatttcctat gaatatttga taaaatataa atgacatcta
     3661 tatgtaaatt catgaaccat gactcttatg aattatcagt gcacaaatta tgtttgatgc
     3721 acacttaagg tcaaaagttt agggtcagtt tatttttttt ttatttttaa ttttatttaa
     3781 taatttaaat aaaattattc tgttcatcaa ggcacattta ttaaatgtaa caataaattg
     3841 ttaaatactt attaaatatt tattttttac ttattgtatg cagtttcaat tgaagtcatt
     3901 attgctttta ttactattac cattaataaa aataacatta ataataataa taataataat
     3961 aataattgat attacagtga tttctgaagg atcacgtgac tgaagactgg agtaatgaag
     4021 ctgaaaactc atcatttaaa tcactggagt aaatgattca attaaatgat catttaatga
     4081 tcattctttt gaacagttat tttatagtgc aataacattt cacagtttta ccatttgttt
     4141 tgtatttttg attcaataaa tgcagccttg gtgagcagga gaagcttaat ttaaaacatt
     4201 taaaactcct actgacccca aacttttgac cggtagtgta catgtattac tgtatgttca
     4261 aactatatgc ttttattcat aagtgtttct attgctgtac atttgactgt ctgcatcagg
     4321 tgaagtgtat gaaagagtgt gtgtttactc caccccgtgg gaaagagcag tagctgcaat
     4381 gtgtgtgtgt gagtagttag tgatgcatga gtgatccact gaaccctgtc cagccccctg
     4441 ctgcagctct caacacatca tgataggtca cctctactgg gaggctttca gcacagctct
     4501 cacaactgat ttatcagaga gtactctgaa atggagaaat tactttcata ttaaagagca
     4561 cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacacc
     4621 tagttcacct tattaacgtg gttaagcctt taaatgtcac tttaagctgt atagaagtgt
     4681 cttgaaaaat atctattcaa atattattta ctgtcatcaa ggaaaagata aaataaatca
     4741 gttattagaa ataagttttt aaaactatta tacttagaaa tgtgctgaaa caatcttcct
     4801 agaacagaac agaaattggg gaaaaaataa acaaaaataa ataaatctat ctatctatag
     4861 tgtgtataat gtgtgtagtg agtatgttta aaataggtca actaatatgt tcataataaa
     4921 aatacagcat caatggtttg ggtgttttta gatccaaagg tcttaaacgt ggttattgct
     4981 ggttgttcag ttttttctag gctctaagaa aaatcatttt agaaactgta actgtttcct
     5041 tatccatctg aattccacct ttgggatgta gtggaacggg agatcaactg catgatgcta
     5101 tcaagtcaat atggatcaaa atctcaggaa tatttccagg accttattga atctatgcca
     5161 tgaaggatta aggcagttct gaaaggggaa atgcggttta acccggtaat agcaaggtgt
     5221 acctaataaa gtggccggtg agtgtatata ttgttattgt ttcaccttca gaaatgacat
     5281 atctagacaa tagtttcctt tttctttttt aattattgta cttctgtaag attcttcaat
     5341 gttcaatggt tttcttatgt taaaggcgca gagctgtagt tttagtgaag taacagcatt
     5401 ccgttgacca aagctgacag atgaaggaga gtcagttctg accatgcagt tgtcagtcac
     5461 aattaagcct ggtttggtta caagttaaac gtagacctgc ttgtggtcag gatcatcctt
     5521 taaaagaact agatttgttt ttaacaattt gtaaccggtg tgctctgaag tgtcttaaat
     5581 aaagcatata ggaagcaaag cagtgaaaaa tgttccttat aatctacgag cattagctta
     5641 aatgtataaa caaaataata ttaattgtgt gttcagatca aatataggta ttgttacacc
     5701 taaaataaac ccattgcttt aaaaaataat taaacacagt gtacaatatt agcatacttc
     5761 ctctgacatc tctaagtaaa acaagagctt tcacgattta ctcactgacc catcttacag
     5821 cacttctctg tgaatttagg ccatcacaaa tgctgtgccc agttctctga ctgggtttga
     5881 cagtcgccca tctgtgtcca gcagtctgat gagctcatcc tctgtaaatc tggctggctg
     5941 ccgtgttgtt ttgtgtctgt ccgtgagtct ctctggcctt catacactcc tctgctactt
     6001 catagactgc tgatgagtgc agaggccaaa ccattagtgc tttccttttt ttagacctca
     6061 gtaatgcaca acaatgcaaa acaagtagat tgagcctttt gttctttgga ctgagcattt
     6121 ggttagaata gcttgaaggg atagttagaa gatattttga agggaaacta aaaacctgtt
     6181 accatttgac atccaaagaa ggaaaactaa tatgattgag tggttacaga tttccaacac
     6241 ttttcaacat atattatttt gtgttccact aaagaaacag tcagatatta tactgtcaac
     6301 caaagtactg cacttgactc aaaaaagctc tgcagcaagt tgcctcttat tctaggtcga
     6361 gtcaaccttt ttttctctct ctctgtcggt tcattcaagg ttcagtaaat gatgacagaa
     6421 ttttcagctt ttattttggg tgaactaacc ctttatatat ttgttgtaag tttatttttc
     6481 tgtatattat aaatatatta gcaacataat tctaagctac acttgtaccc aatcaagggt
     6541 cacactttat tttgatggta tgtttgtttg aatttaagtt acattgcatc tacatgtcaa
     6601 ctaattctca ttagattata agaagactgt taggttgggg ctagtgtaag ttgacatgta
     6661 cttgcaaagt ttctcagagt cagttaaatg tctgttttgt agcagtatta acagatatta
     6721 agcacactgt atattaatac tcaaatgaac cgtcaaaata aagtgttacc gtaaaaatta
     6781 tttccacatc tcaaggtagc tttatgagga aagttttctt taagattagt ttcttcgaac
     6841 aaacttgcat gttaaatatg tgctcagata tggggcacac agacaaaatg tttattggac
     6901 gaataacccc tttaacagtt aaccagcatt tttaaaggga tagtcagaag atattttgaa
     6961 ggaaactaaa aacctgtaac cattcgacaa tcgaagaagg taaaagaaat atgatagagt
     7021 agttacagtt tttcaacact ctttataata tattgtttag tattcaactg aagaaatagt
     7081 caaacacact gtaaaaaaag ttggtttaaa ttgactcaac tttcaactca agtatagtac
     7141 ttgacttgat ttaagttgag ttaactagac taaactaaaa gcactaaaag cactgcggca
     7201 agtcgccttt caattttagg ttgagtaaat tattattatt tttttttaca gttcaggttc
     7261 agtaaatgat aacataattt tcagttttgg gtgaactatc cctttatata tttgttgtaa
     7321 gttaattttt ctgtaaatta taaattaatt agcaaaaaag ctgcacttct tcaccattaa
     7381 ttaattaata catctcaaag tagctttata aggaaagttt tctttaatta tgtttctttg
     7441 catgttaaaa ttgtgctcag atatggagca cacaagcaaa atgtttattg gatgaataac
     7501 ccctttaaca tttaaccagc atgagttcaa gtaaacaaat caattcctat gatttttctg
     7561 ctaaaccgat ataaatcagt gctgctgaag agcatgtgtg tgatgttctc agatcagcac
     7621 aaaagaggga gcgccagttt cttttaataa tgaagaatta cgtaatcacc tcctgtgttc
     7681 cctgaaacca ttaaactccc agacagaaca aaactgtgac tgagaaagca gagcaactcc
     7741 ggagtaactc agaagatctg caaaccaact ctttagcatg aggtaagaga gaattaaagt
     7801 ccaatatcag tgctgatgcc atctcagtct ctctatttct gtgttccagc agggtatgaa
     7861 taggattttc tctggctcac tgtgaaaaga ggcagtcagg agactggctg gacaagcaca
     7921 aacaattgcg ggctcacaaa caccccctcg gcctttgccc tccccatcag accccccctc
     7981 tctctttcac cccccacatc aactctataa aaacccagag ttcacccgca ctcgatctca
     8041 ttctcctcca ccatggagtc ccagcgttcc tcgtcgacgg taccgcgggc ccgggatcca
     8101 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     8161 ttaattcaag tttgtacaaa aaagttgaac gagaaacgta aaatgatata aatatcaata
     8221 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc
     8281 agtcactatg aaccaactac ttagatggta ttagtgacct gtagaattcg agctctagag
     8341 ctgcagggcg gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     8401 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     8461 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     8521 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     8581 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     8641 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     8701 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     8761 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     8821 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     8881 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     8941 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     9001 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     9061 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     9121 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     9181 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     9241 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     9301 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     9361 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     9421 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9541 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9601 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     9661 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9721 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9781 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9841 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9901 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9961 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10021 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10081 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    10141 gattctgtgg ataaccgtat taccgccttt gagtgagctg ata
//
