LOCUS       sequence-450095-       10260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti wt hTdT template for library 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Carlson CK, Loveless TB, Milisavljevic M, Kelly PI, Mills JH, Tyo 
            KEJ, Liu CC
  TITLE     A massively parallel in vivo assay of TdT mutants yields variants 
            with altered nucleotide insertion biases.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 11:2024.06.11.598561. doi: 
            10.1101/2024.06.11.598561.
  PUBMED    38915690
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 11:2024.06.11.598561. doi: 
            10.1101/2024.06.11.598561."
COMMENT     Sequence Label: pCKC014
FEATURES             Location/Qualifiers
     source          1..10260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(233..277)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    462..695
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1188..1313
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1360..1540
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1541..1767)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1795..1815)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1795..1813)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1834..1850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1834..1850)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1847..1869)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1858..1874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1882..1888,1889..1906,1907..1912))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1927..1948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2065..2082)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2236..2824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2316..2335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2995..3789)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2995..3789)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3106..3125)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3716..3735
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3790..3894)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3920..4375)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4057..4078)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4269..4288
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4502..4524
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4516..4533
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4517..4533
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4543..4561
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4582..4717
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4757..4878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4768..4787)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4822..4841
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4950..5183
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(5270..5869)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5353..5373)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5850..5869
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(5870..5926)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5930..6643)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(6670..7169)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6718..6737)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7111..7134
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    7268..7856
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7374..7385
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7783..7803
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      9384..9393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9423..9441)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9486..9689)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9515..9535)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9690..10068
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    10110..10226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
       61 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
      121 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
      181 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
      241 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
      301 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
      361 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
      421 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
      481 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
      541 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
      601 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
      661 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
      721 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
      781 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
      841 tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
      901 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
      961 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     1021 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
     1081 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     1141 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     1201 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     1261 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     1321 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     1381 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     1441 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     1501 agagctccca ggctcagatc tggtctaacc agagagaccc gtttatgtat cgagctaggc
     1561 acttaaatac aatatctctg caatgcggca attcagtggt tcgtccaatc catgtcagac
     1621 ccgtctgttg ccttcctaat aaggcacgat cgtaccacct tacttccacc aatcggcatg
     1681 cacggtgctt tttctctcct tgtaaggcat gttgctaact catcgttacc atgttgcaag
     1741 actacaagag tattgcataa gactacatta agcttgcagc tccagctttt gttcccttta
     1801 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     1861 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     1921 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1981 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2041 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2101 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2161 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2221 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2281 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2341 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2401 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2461 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2521 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2581 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2641 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2701 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2761 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2821 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2881 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2941 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagtcagaa
     3001 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     3061 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     3121 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     3181 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     3241 atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc
     3301 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     3361 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcaa gtagccggat caagcgtatg
     3421 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     3481 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     3541 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     3601 ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     3661 cccctgcgct gacagacgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     3721 gtcatagccg aatagcctct ccacccaagc ggccggagaa ccagcgtgca atccatcttg
     3781 ttcaatcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3841 gagcggatac atatttaaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3901 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     3961 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     4021 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     4081 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     4141 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     4201 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     4261 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     4321 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     4381 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     4441 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     4501 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     4561 ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     4621 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     4681 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggatag
     4741 ctagagccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4801 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4861 agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa gttataaata
     4921 ctgaataata agatgacatg aactactact gctagagatt ttccacactg actaaaaggg
     4981 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     5041 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     5101 ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg
     5161 ttgggagtga gctagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     5221 agctgccttg taagtcattg gtcttaaagg taccgagctc gaattgtgct caggcaccgg
     5281 gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg
     5341 tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg
     5401 gcaccccggc gcgctcggcc gcctccactc cggggagcac gacggcgctg cccagaccct
     5461 tgccctggtg gtcgggcgag actccgacgg tggccaggaa ccacgcgggc tccttgggcc
     5521 ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc cagccgggaa ccgctcaact
     5581 cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg
     5641 tccagaccgc caccgcggcg ccgtcgtccg cgacccacac cttgccgatg tcgagcccga
     5701 cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccgggtcga
     5761 cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggcccggg
     5821 ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gggccggggt
     5881 tctcctccac gtcgccggcc tgcttcagca gggagaagtt ggtggcgcct ttgtacagtt
     5941 catccatacc atgcgtgatg cccgctgcgg ttacgaactc cagcagaacc atatgatcgc
     6001 gtttctcgtt cggatcttta gacagaacgc tttgcgtgct cagatagtga ttgtctggca
     6061 gcagaacagg accatcaccg attggagtgt tttgctggta gtgatcagcc agctgcacgc
     6121 tgccatcctc cacgttgtgg cgaattttaa aattcgcttt aatgccattt ttttgtttat
     6181 cggcggtgat gtaaacattg tggctgttaa aattgtattc cagcttatgg cccaggatat
     6241 tgccgtcctc tttaaagtca atgcctttca gctcaatgcg gtttaccagg gtatcgcctt
     6301 caaatttcac ttccgcacgc gttttgtacg tgccgtcatc cttaaaggaa atcgtgcgtt
     6361 cctgcacata gccttccggc atggcggact tgaagaagtc atgctgcttc atatggtccg
     6421 gataacgagc aaagcactga acaccataag tcagcgtcgt taccagagtc ggccaaggaa
     6481 ccggcagttt accagtagta cagatgaact tcagcgtcag tttaccatta gttgcgtcac
     6541 cttcaccctc gccacgcacg gaaaacttat gaccgttgac atcaccatcc agttccacca
     6601 gaatagggac gacaccagtg aacagctctt cgcctttacg catggtggcg accggtagcg
     6661 ctaccaggtc gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt
     6721 ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag
     6781 cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa
     6841 ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt
     6901 gctgtccatc tgcacgagac tagtgatacg tgctacttcc atttgtcacg tcctgcacga
     6961 cgcgagctgc ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa
     7021 ggggccacca aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg
     7081 ccagaggcca cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga
     7141 ctgccttggg aaaagcgcct cccctacccg gtagatgcat ggcggtaata cggttatcca
     7201 cgcggccgcc taatagatcc cttcctttcc tctgccctca cgtgctcagt ctgggccgga
     7261 tccccaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga
     7321 cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg
     7381 gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc
     7441 agccatggaa aggacgtcag cttccccgac aacaccacgg aattgtcagt gcccaacagc
     7501 cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg
     7561 agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg
     7621 ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac
     7681 tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg
     7741 gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca
     7801 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattgcta
     7861 ggcatttctt tcccacggtt caatataatc caatcccaga tgcgcaaaaa tttcttcttc
     7921 actttctgct ttgaggaata tcctcttggt cttgtcatat aaagcatggt tatccagaat
     7981 catcttccgc tcatgtgtgg catagcgccg gaggtctctc tcaaactgcc gggatccagt
     8041 ccatcccaac agggcaaagg cacgacgctc gtaggggcac agaactaaat ccacacggat
     8101 ggccttccag gtctttcctt cctgccagct ggactggtca ctgtccactc tttgacgagg
     8161 caatttgaaa atcagaaagc acttttgaaa atgatccaaa gcatcaacct tcctgctagg
     8221 caacctgagc ttttcaaatg ttgactccac aaggtcataa tataaaagta atcccttctt
     8281 ttcccataag ttcatcactt tctgtaaaag ttgctcttca tcctctgttg atcctgggct
     8341 ggtaattaaa aaatctacat catgccccat cttcttaccc ctccggaacc ctcctgtcat
     8401 ggtgacgaaa gcatccggaa gaaatgccca gacagcctct ttaaccagca cactgacggc
     8461 ctctgcttct gccctggtca cacagctgac aaggtcttca taatacagaa atcctgcttt
     8521 ctgcattcgt gtaaatttca ggcttttgtc cgaccttact ttactcagag ttctgaaacc
     8581 catcctgaac cacttctcag aagtcttcag ccccactcca aatacagaag taaagagttt
     8641 gaaggattga tatcgttcat catttaacac agctttaact tcagaacttt ctccatcttc
     8701 aataatctcc tctatgatac ccttcacctt ggaccccagg cagggaattc cttctgtgtc
     8761 cttcatactg atgattgtga atggcagaga tttcaataca gaagctgctc tcataaatgt
     8821 cacacaggag tcttcatttt ctctaaactc acagttttca gccagtatat caaaggcatc
     8881 cgtgaatatc tggttacagt tgtttaaagt ggttcttctc tgacacgcat actgggagat
     8941 cttttgtaca gcaattggtg gagtcttcgg ggggcctggg ttggtgctat ctgaatagtc
     9001 tcttctcaca acaagctggt gttttcctgt catttccacc ggtttccctg ctcctatgca
     9061 ttcgatcagc caggagacat cgaggagctc tggttgtgag ctgacttgta ctttctgtgc
     9121 ttgaagccac tccagaacat ccgaacccga gttgttctct gctacaatgt gggtgacaga
     9181 atcactgagc tcattttcaa ccctgaaccc tttcctgcgg gccagctcca tgaggaacgc
     9241 tctgcgggtg gttcccattt tcttctccaa aatgaagacg accaaatctt gaaatttgat
     9301 gtcttgagga gaggaggcca tcaaggcacc cgtctgccgg ggtctcttct tccgagggct
     9361 caagtgggac gctcgtggtg ggtccatggt ggcgaccggt agcgctagcc agcttgggtc
     9421 tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc
     9481 cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
     9541 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
     9601 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
     9661 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
     9721 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     9781 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     9841 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     9901 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     9961 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
    10021 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtta ccgtaagtta
    10081 tgtaacgcgg aactcccaag cttatcgata aaattttgaa ttttgtaatt tgtttttgta
    10141 attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc
    10201 ccccaatccc cccttttctt ttaaaagtta accgataccg tcgagatccg ttcactaatc
//
