LOCUS       sequence-450096-       10266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti wt hTdT template for libraries 1-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Carlson CK, Loveless TB, Milisavljevic M, Kelly PI, Mills JH, Tyo 
            KEJ, Liu CC
  TITLE     A massively parallel in vivo assay of TdT mutants yields variants 
            with altered nucleotide insertion biases.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 11:2024.06.11.598561. doi: 
            10.1101/2024.06.11.598561.
  PUBMED    38915690
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 11:2024.06.11.598561. doi: 
            10.1101/2024.06.11.598561."
COMMENT     Sequence Label: pCKC017
FEATURES             Location/Qualifiers
     source          1..10266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             565..621
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             622..1221
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(622..641)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1118..1138
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1308..1541
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1613..1734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1650..1669)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1704..1723
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1774..1909
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1930..1948)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1958..1975)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1958..1974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1967..1989)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2116..2571
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2203..2222)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2413..2434
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2597..2701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2702..3496
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2702..3496
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2756..2775)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3366..3385
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      3667..4255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4156..4175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4409..4426
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4543..4564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4579..4584,4585..4602,4603..4609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4617..4633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4622..4644
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4641..4657
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4641..4657
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4676..4696
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4678..4696
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4724..4950
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4951..5131
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5178..5303
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5796..6029
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6214..6258
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6525..6641
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6764..7067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7068..7271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7222..7242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        7316..7334
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7364..7373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8901..9489
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8954..8974)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9372..9383)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9588..10087
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9623..9646)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10020..10039
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(10114..10266,1..561)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aaactgccgg ttccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt
       61 gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc
      121 tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa
      181 gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa
      241 gaggacggca atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac
      301 atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg
      361 gaggatggca gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt
      421 cctgttctgc tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg
      481 aacgagaaac gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat
      541 ggtatggatg aactgtacaa aggcgccacc aacttctccc tgctgaagca ggccggcgac
      601 gtggaggaga accccggccc catgaccgag tacaagccca cggtgcgcct cgccacccgc
      661 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
      721 cgccacaccg tggacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
      781 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
      841 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
      901 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
      961 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac
     1021 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     1081 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     1141 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     1201 acccgcaagc ccggtgcctg agcacaattc gagctcggta cctttaagac caatgactta
     1261 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     1321 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     1381 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     1441 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     1501 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     1561 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     1621 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     1681 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1741 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     1801 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     1861 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     1921 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     1981 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     2041 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     2101 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2161 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2221 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2281 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     2341 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2401 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     2461 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     2521 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     2581 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac atttaaatat
     2641 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2701 tatgattgaa caagatggat tgcacgctgg ttctccggcc gcttgggtgg agaggctatt
     2761 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccgtctgtc
     2821 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     2881 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     2941 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3001 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3061 gcggcggctg catacgcttg atccggctac ttgcccattc gaccaccaag cgaaacatcg
     3121 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3181 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     3241 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     3301 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     3361 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     3421 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     3481 tgacgagttc ttctgactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3541 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3601 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3661 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3721 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3781 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3841 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3901 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3961 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4021 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4081 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4141 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4201 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4261 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4321 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4381 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4441 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     4501 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     4561 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     4621 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     4681 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     4741 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     4801 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     4861 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     4921 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     4981 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5041 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5101 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     5161 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     5221 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     5281 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     5341 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     5401 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     5461 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     5521 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     5581 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     5641 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     5701 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     5761 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     5821 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     5881 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     5941 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     6001 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     6061 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     6121 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     6181 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     6241 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     6301 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     6361 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     6421 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     6481 agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg
     6541 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     6601 actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag cttgggagtt
     6661 ccgcgttaca taacttacgg taactgcagc attgattatt gactagttat taatagtaat
     6721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     6781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     6841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     6901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     6961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     7021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     7081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     7141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     7201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     7261 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
     7321 cgactcacta tagggagacc caagctggct agcgctaccg gtcgccacca tggacccacc
     7381 acgagcgtcc cacttgagcc ctcggaagaa gagaccccgg cagacgggtg ccttgatggc
     7441 ctcctctcct caagacatca aatttcaaga tttggtcgtc ttcattttgg agaagaaaat
     7501 gggaaccacc cgcagagcgt tcctcatgga gctggcccgc aggaaagggt tcagggttga
     7561 aaatgagctc agtgattctg tcacccacat tgtagcagag aacaactcgg gttcggatgt
     7621 tctggagtgg cttcaagcac agaaagtaca agtcagctca caaccagagc tcctcgatgt
     7681 ctcctggctg atcgaatgca taggagcagg gaaaccggtg gaaatgacag gaaaacacca
     7741 gcttgttgtg agaagagact attcagatag caccaaccca ggccccccga agactccacc
     7801 aattgctgta caaaagatct cccagtatgc gtgtcagaga agaaccactt taaacaactg
     7861 taaccagata ttcacggatg cctttgatat actggctgaa aactgtgagt ttagagaaaa
     7921 tgaagactcc tgtgtgacat ttatgagagc agcttctgta ttgaaatctc tgccattcac
     7981 aatcatcagt atgaaggaca cagaaggaat tccctgcctg gggtccaagg tgaagggtat
     8041 catagaggag attattgaag atggagaaag ttctgaagtt aaagctgtgt taaatgatga
     8101 acgatatcaa tccttcaaac tctttacttc tgtatttgga gtggggctga agacttctga
     8161 gaagtggttc aggatgggtt tcagaactct gagtaaagta aggtcggaca aaagcctgaa
     8221 atttacacga atgcagaaag caggatttct gtattatgaa gaccttgtca gctgtgtgac
     8281 cagggcagaa gcagaggccg tcagtgtgct ggttaaagag gctgtctggg catttcttcc
     8341 ggatgctttc gtcaccatga caggagggtt ccggaggggt aagaagatgg ggcatgatgt
     8401 agatttttta attaccagcc caggatcaac agaggatgaa gagcaacttt tacagaaagt
     8461 gatgaactta tgggaaaaga agggattact tttatattat gaccttgtgg agtcaacatt
     8521 tgaaaagctc aggttgccta gcaggaaggt tgatgctttg gatcattttc aaaagtgctt
     8581 tctgattttc aaattgcctc gtcaaagagt cgacagtgac cagtccagct ggcaggaagg
     8641 aaagacctgg aaggccatcc gtgtggattt agttctgtgc ccctacgagc gtcgtgcctt
     8701 tgccctgttg ggatggactg gatcccggca gtttgagaga gacctccggc gctatgccac
     8761 acatgagcgg aagatgattc tggataacca tgctttatat gacaagacca agaggatatt
     8821 cctcaaagca gaaagtgaag aagaaatttt tgcgcatctg ggattggatt atattgaacc
     8881 gtgggaaaga aatgcctagc aatcaacctc tggattacaa aatttgtgaa agattgactg
     8941 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     9001 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     9061 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     9121 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     9181 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     9241 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     9301 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     9361 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     9421 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     9481 cctccccgcc tggggatccg gcccagactg agcacgtgag ggcagaggaa aggaagggat
     9541 ctattaggcg gccgcgtgga taaccgtatt accgccatgc atctaccggg taggggaggc
     9601 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     9661 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     9721 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     9781 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgta tcactagtct
     9841 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     9901 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     9961 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
    10021 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
    10081 cctttcgacc tggtagcgct accggtcgcc accatgcgta aaggcgaaga gctgttcact
    10141 ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg
    10201 cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt catctgtact
    10261 actggt
//
