LOCUS       sequence-450109-        8070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8070)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Desmoglein2-C-18
FEATURES             Location/Qualifiers
     source          1..8070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1320
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(758..776)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1026..1045
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4858..4979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4895..4914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4949..4968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4986..5441)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5123..5144)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5335..5354
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5468..5572
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5574..5931
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5574..5765
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5597..5617)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5782..5917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5966..6760
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5966..6760
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6020..6039)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6630..6649
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6948..6967)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6992..7039
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7368..7956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7857..7876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggaagtg gagctgggag cggggcaagc ggaagcggaa gtggctccgg tggggcgcgg
     1381 agcccgggac gcgcgtacgc cctgctgctt ctcctgatct gctttaacgt tggaagtgga
     1441 cttcacttac aggtcttaag cacaagaaat gaaaataagc tgcttcctaa acatcctcat
     1501 ttagtgcggc aaaagcgcgc ctggatcacc gcccccgtgg ctcttcggga gggagaggat
     1561 ctgtccaaga agaatccaat tgccaagata cattctgatc ttgcagaaga aagaggactc
     1621 aaaattactt acaaatacac tggaaaaggg attacagagc caccttttgg tatatttgtc
     1681 tttaacaaag atactggaga actgaatgtt accagcattc ttgatcgaga agaaacacca
     1741 ttttttctgc taacaggtta cgctttggat gcaagaggaa acaatgtaga gaaaccctta
     1801 gagctacgca ttaaggttct tgatatcaat gacaacgaac cagtgttcac acaggatgtc
     1861 tttgttgggt ctgttgaaga gttgagtgca gcacatactc ttgtgatgaa aatcaatgca
     1921 acagatgcag atgagcccaa taccctgaac tcgaaaattt cctatagaat cgtatctctg
     1981 gagcctgctt atcctccagt gttctaccta aataaagata caggagagat ttatacaacc
     2041 agtgttacct tggacagaga ggaacacagc agctacactt tgacagtaga agcaagagat
     2101 ggcaatggag aagttacaga caaacctgta aaacaagctc aagttcagat tcgtattttg
     2161 gatgtcaatg acaatatacc tgtagtagaa aataaagtgc ttgaagggat ggttgaagaa
     2221 aatcaagtca acgtagaagt tacgcgcata aaagtgttcg atgcagatga aataggttct
     2281 gataattggc tggcaaattt tacatttgca tcaggaaatg aaggaggtta tttccacata
     2341 gaaacagatg ctcaaactaa cgaaggaatt gtgaccctta ttaaggaagt agattatgaa
     2401 gaaatgaaga atcttgactt cagtgttatt gtcgctaata aagcagcttt tcacaagtcg
     2461 attaggagta aatacaagcc tacacccatt cccatcaagg tcaaagtgaa aaatgtgaaa
     2521 gaaggcattc attttaaaag cagcgtcatc tcaatttatg ttagcgagag catggataga
     2581 tcaagcaaag gccaaataat tggaaatttt caagcatttg atgaggacac tggactacca
     2641 gcccatgcaa gatatgtaaa attagaagat agagataatt ggatctctgt ggattctgtc
     2701 acatctgaaa ttaaacttgc aaaacttcct gattttgaat ctagatatgt tcaaaatggc
     2761 acatacactg taaagattgt ggccatatca gaagattatc ctagaaaaac catcactggc
     2821 acagtcctta tcaatgttga agacatcaac gacaactgtc ccacactgat agagcctgtg
     2881 cagacaatct gtcacgatgc agagtatgtg aatgttactg cagaggacct ggatggacac
     2941 ccaaacagtg gccctttcag tttctccgtc attgacaaac cacctggcat ggcagaaaaa
     3001 tggaaaatag cacgccaaga aagtaccagt gtgctgctgc aacaaagtga gaaaaagcta
     3061 gggagaagtg aaattcagtt cctgatttca gacaatcagg gttttagttg tcctgaaaag
     3121 caggtcctta cactcacagt ttgtgagtgt ctgcatggca gcggctgcag ggaagcacag
     3181 catgactcct atgtgggcct gggacccgca gcaattgcgc tcatgatttt ggcctttctg
     3241 ctcctgctat tggtgccact tttactgctg atgtgccatt gcggaaaggg cgccaaaggc
     3301 tttaccccca tacctggcac catagagatg ctgcatcctt ggaataatga aggagcacca
     3361 cctgaagaca aggtggtgcc atcatttctg ccagtggatc aagggggcag tctagtagga
     3421 agaaatggag taggaggtat ggccaaggaa gccacgatga aaggaagtag ctctgcttcc
     3481 attgtcaaag ggcaacatga gatgtccgag atggatggaa ggtgggaaga acacagaagc
     3541 ctgctttctg gtagagctac ccagtttaca ggggccacag gcgctatcat gaccactgaa
     3601 accacgaaga ccgcaagggc cacaggggct tccagagaca tggccggagc tcaggcagct
     3661 gctgttgcac tgaacgaaga gttcttaaga aattatttca ctgataaagc ggcctcttac
     3721 actgaggaag atgaaaatca cacagccaaa gattgccttc tggtttattc tcaggaagaa
     3781 actgaatcgc tgaatgcttc tattggttgt tgcagtttta ttgaaggaga gctagatgac
     3841 cgcttcttag atgatttggg acttaaattc aagacactag ctgaagtttg cctgggtcaa
     3901 aaaatagata taaataagga aattgagcag agacaaaaac ctgccacaga aacaagtatg
     3961 aacacagctt cacattcact ctgtgagcaa actatggtta attcagagaa tacctactcc
     4021 tctggcagta gcttcccagt tccaaaatct ttgcaagaag ccaatgcaga gaaagtaact
     4081 caggaaatag tcactgaaag gtctgtgtct tctaggcagg cgcaaaaggt agctacacct
     4141 cttcctgacc caatggcttc tagaaatgtg atagcaacag aaacttccta tgtcacaggg
     4201 tccactatgc caccaaccac tgtgatcctg ggtcctagcc agccacagag ccttattgtg
     4261 acagagaggg tgtatgctcc agcttctacc ttggtagatc agccttatgc taatgaaggt
     4321 acagttgtgg tcactgaaag agtaatacag cctcatgggg gtggatcgaa tcctctggaa
     4381 ggcactcagc atcttcaaga tgtaccttac gtcatggtga gggaaagaga gagcttcctt
     4441 gcccccagct caggtgtgca gcctactctg gccatgccta atatagcagt aggacagaat
     4501 gtgacagtga cagaaagagt tctagcacct gcttccactc tgcaatccag ttaccagatt
     4561 cccactgaaa attctatgac ggctaggaac accacggtgt ctggagctgg agtccctggc
     4621 cctctgccag attttggttt agaggaatct ggtcattcta attctaccat aaccacatct
     4681 tccaccagag ttaccaagca tagcactgta cagcattctt actcctaagg atccaccgga
     4741 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     4801 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     4861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     4981 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     5041 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     5101 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     5161 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     5221 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     5281 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     5341 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     5401 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     5461 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5521 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     5581 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5641 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5701 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5761 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5821 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5881 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     5941 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     6001 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6061 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6121 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     6181 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6241 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6301 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6361 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6421 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6481 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6541 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6601 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6661 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6721 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     6781 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     6841 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6901 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     6961 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     7021 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     7081 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     7141 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     7201 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7261 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7321 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7501 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7741 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7801 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7861 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7921 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7981 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8041 tctgtggata accgtattac cgccatgcat
//
