LOCUS       sequence-45013-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(758-1057)
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..70
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaggagatat accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc
       61 gcgcggcagc catatgttgc aaactgaaag atctgtagag agtagcagta tttcattggt
      121 acctggtact gattatggca ctcaggaaag tatctcgtta ctggaagtta gcactctagg
      181 gaaggcaaaa acagaaccaa ataaatgtgt gagtcagtgt gcagcatttg aaaaccccaa
      241 gggactaact catggttgtt ccaaagataa tagaaatgac acagaaggct ttaagtatcc
      301 attgggacat gaagttaacc acagtcggga aacaagcata gaaatggaag aaagtgaact
      361 tgatgctcag tatttgcaga atacattcaa ggtttcaaag cgccagtcat ttgctccgtt
      421 ttcaaatcca ggaaatgcag aagaggaatg tgcaacattc tctgcccact ctgggtcctt
      481 aaagaaacaa agtccaaaag tcacttttga atgtgaacaa aaggaagaaa atcaaggaaa
      541 gaatgagtct aatatcaagc ctgtacagac agttaatatc actgcaggct ttcctgtggt
      601 tggtcagaaa gataagccag ttgataatgc caaatgtagt atcaaaggag gctctaggtt
      661 ttgtctatca tctcagttca gaggcaacga aactggactc attactccaa ataaacatgg
      721 acttttacaa aacccatatc gtataccacc actttttccc atcaagtcat ttgttaaaac
      781 taaatgtaag aaaaatctgc tagaggaaaa ctttgaggaa cattcaatgt ca
//
