LOCUS       sequence-450133-       11854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For inducible expression and cytosolic localization of Dam.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Papathanasiou S, Mynhier NA, Liu S, Brunette G, Stokasimov E, Jacob 
            E, Li L, Comenho C, van Steensel B, Buenrostro JD, Zhang CZ, Pellman
            D
  TITLE     Heritable transcriptional defects from aberrations of nuclear 
            architecture.
  JOURNAL   Nature. 2023 Jul;619(7968):184-192. doi: 10.1038/s41586-023-06157-7.
            Epub 2023 Jun 7.
  PUBMED    37286600
REFERENCE   2  (bases 1 to 11854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06157-7"; journalName: "Nature"; date: 
            "2023-07"; volume: "619"; issue: "7968"; pages: "184-192"
COMMENT     Sequence Label: 3×Cherry Dam AID Smash
FEATURES             Location/Qualifiers
     source          1..11854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2805..2922
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2976..3290
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2984..3002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3020..3038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3055..3073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3091..3109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3127..3145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3162..3180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3198..3216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3263..3287
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3311..3335
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4193..4900
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             4919..5626
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             5645..6352
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             6359..6562
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             6629..6652
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(7491..7515)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7533..7550
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        7560..8070
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7989..8007
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8080..8676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8080..8099)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8576..8596
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             8677..8730
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8731..9348,9349..9354,9355..9390,9391..9393,9394..9429,
                     9430..9432,9433..9468,9469..9477)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9517..9537)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9935..9946)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10151..10384
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10456..10577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10493..10512)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10547..10566
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10617..10752
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10752..10823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10824..10892,10893..11684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11042..11061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2821 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2881 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2941 agcagagatc cactttggcc gcgaatcgat atgtcgagtt tactccctat cagtgataga
     3001 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
     3061 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     3121 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
     3181 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
     3241 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagaattg
     3301 gctagcatca acaagtttgt acaaaaaagc aggcttcatg aagaaaaatc gcgctttttt
     3361 gaagtgggca gggggcaagt atcccctgct tgatgatatt aaacggcatt tgcccaaggg
     3421 cgaatgtctg gttgagcctt ttgtaggtgc cgggtcggtg tttctcaaca ccgacttttc
     3481 tcgttatatc cttgccgata tcaatagcga cctgatcagt ctctataaca ttgtgaagat
     3541 gcgtactgat gagtacgtac aggccgcacg cgagctgttt gttcccgaaa caaattgcgc
     3601 cgaggtttac tatcagttcc gcgaagagtt caacaaaagc caggatccgt tccgtcgggc
     3661 ggtactgttt ttatatttga accgctacgg ttacaacggc ctgtgtcgtt acaatctgcg
     3721 cggtgagttt aacgtgccgt tcggccgcta caaaaaaccc tatttcccgg aagcagagtt
     3781 gtatcacttc gctgaaaaag cgcagaatgc ctttttctat tgtgagtctt acgccgatag
     3841 catggcgcgc gcagatgatg catccgtcgt ctattgcgat ccgccttatg caccgctgtc
     3901 tgcgaccgcc aactttacgg cgtatcacac aaacagtttt acgcttgaac aacaagcgca
     3961 tctggcggag atcgccgaag gtctggttga gcgccatatt ccagtgctga tctccaatca
     4021 cgatacgatg ttaacgcgtg agtggtatca gcgcgcaaaa ttgcatgtcg tcaaagttcg
     4081 acgcagtata agcagcaacg gcggcacacg taaaaaggtg gacgaactgc tggctttgta
     4141 caaaccagga gtcgtttcac ccgcgaaaaa attaattaaa cgatccggaa gtatggtgag
     4201 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     4261 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     4321 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     4381 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     4441 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     4501 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tcactgcagg acggcgagtt
     4561 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     4621 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     4681 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     4741 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4801 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgctga
     4861 gggccgccac tccaccggcg gcatggacga gctgtacaag tctggcggcc gctctggcat
     4921 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     4981 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     5041 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     5101 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     5161 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     5221 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctcac tgcaggacgg
     5281 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     5341 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     5401 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     5461 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     5521 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     5581 cgctgagggc cgccactcca ccggcggcat ggacgagctg tacaagggta gcatcgatgg
     5641 tagcatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     5701 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     5761 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     5821 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     5881 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     5941 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctcactgca
     6001 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     6061 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     6121 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     6181 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     6241 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     6301 cgaacgcgct gagggccgcc actccaccgg cggcatggac gagctgtaca agctcgagaa
     6361 ggagaaatca gcctgcccta aagaccccgc aaagcccccc gcaaaagcac aggtggtggg
     6421 atggcccccc gtgcgcagct accggaagaa cgtgatggtg tcctgccaga agagctccgg
     6481 aggaccagag gcagcagcct ttgtgaaggt gtctatggat ggcgcccctt acctgagaaa
     6541 gatcgacctg aggatgtata aggcctccga tgagatggag gagtgttctc agcacctgcc
     6601 tggagcagga tctagcggcg acatcatgga ttacaaggac gatgacgata agggatcctc
     6661 tggaacagga agcggatccg gcacaagcgc cccaatcacc gcctatgcac agcagaccag
     6721 gggactgctg ggatgcatca tcacatccct gaccggaagg gacaagaacc aggtggaggg
     6781 agaggtgcag atcgtgtcta ccgccacaca gaccttcctg gccacatgca tcaatggcgt
     6841 gtgctgggcc gtgtaccacg gagcaggaac aaggaccatc gcatccccaa agggacctgt
     6901 gatccagatg tataccaatg tggaccagga tctggtggga tggccagcac cacagggatc
     6961 tagaagcctg acaccatgca cctgtggcag ctccgacctg tacctggtga caaggcacgc
     7021 cgatgtgatc cccgtgcgga gaaggggcga ctccagaggc tctctgctga gccctaggcc
     7081 aatcagctat ctgaagggct ctagcggagg accactgctg tgcccagcag gacacgccgt
     7141 gggcctgttc cgcgccgccg tgtgcacccg gggcgtggcc aaggccgtgg acttcatccc
     7201 tgtggagaac ctggagacca caatgcggtc cccagtgttc acagacaatt cctctccccc
     7261 tgccgtgaca ctgacccacc ctatcacaaa gatcgatacc aagtacatca tgacctgtat
     7321 gtctgccgac ctggaggtgg tgacaagcac ctgggtgctg gtgggaggcg tgctggccgc
     7381 cctggcagca tattgcctga gcaccggctg cgtggtcatc gtggggagaa tcgtgctgag
     7441 cggaaaacct gctatcatcc ctgaccgaga agtcctgtat taaagatctg acccagcttt
     7501 cttgtacaaa gtggtttagt aatgaaccgg tccaccacca ccaccaccac taaggatccg
     7561 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     7621 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     7681 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     7741 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     7801 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     7861 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     7921 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     7981 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     8041 gttgaccgaa tcaccgacct ctctccccag caattcacca tgaccgagta caagcccacg
     8101 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8161 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8221 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8281 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8341 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8401 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8461 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8521 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     8581 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8641 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccgaag gtagaggttc tctcctcact
     8701 tgtggtgatg ttgaagaaaa ccctggtcca atgtctagac tggacaagag caaagtcata
     8761 aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc
     8821 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     8881 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg
     8941 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc
     9001 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     9061 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     9121 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     9181 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     9241 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     9301 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac
     9361 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     9421 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta
     9481 agtaaggatc gatccaagat atcgtattct taactatgtt gctcctttta cgctatgtgg
     9541 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     9601 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     9661 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     9721 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     9781 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     9841 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     9901 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     9961 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
    10021 gagtcggatc tccctttggg ccgcctcccc gcctgtttcg cctcggcgtc cggactagag
    10081 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
    10141 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
    10201 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    10261 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    10321 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10381 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
    10441 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10501 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10561 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
    10621 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    10681 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    10741 tttttggagg cctttcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10801 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    10861 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    10921 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10981 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    11041 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    11101 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    11161 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    11221 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    11281 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    11341 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    11401 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    11461 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    11521 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    11581 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    11641 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    11701 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11761 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11821 cgtcagaccc cgtagaaaag atcaaaggat cttc
//
