LOCUS       sequence-450135-       10851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of LacI–SNAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Papathanasiou S, Mynhier NA, Liu S, Brunette G, Stokasimov E, Jacob 
            E, Li L, Comenho C, van Steensel B, Buenrostro JD, Zhang CZ, Pellman
            D
  TITLE     Heritable transcriptional defects from aberrations of nuclear 
            architecture.
  JOURNAL   Nature. 2023 Jul;619(7968):184-192. doi: 10.1038/s41586-023-06157-7.
            Epub 2023 Jun 7.
  PUBMED    37286600
REFERENCE   2  (bases 1 to 10851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06157-7"; journalName: "Nature"; date: 
            "2023-07"; volume: "619"; issue: "7968"; pages: "184-192"
COMMENT     Sequence Label: CuO-LacI-NLS–SNAPf
FEATURES             Location/Qualifiers
     source          1..10851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      1024..1159
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1199..1320
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1210..1229)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1264..1283
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             1392..1625
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1699..2287
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1805..1816
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2214..2234
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2307..2906)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2390..2410)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2887..2906
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(2913..2966)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3003..3611)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     CDS             complement(3723..3749)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(3729..3755)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3758..3767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             3795..4063
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(4076..4287)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             complement(4357..4902)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             complement(4921..4941)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     5983..6002
                     /label=LacI-R
                     /note="LacI, reverse primer"
     regulatory      6021..6030
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        6920..7223
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7294..7410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7677..7721)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7906..8139
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8632..8757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8804..8984
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8985..9211)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9226..9243)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9226..9242)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9235..9257)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9451..9470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9570..9592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9630..9648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9716..9820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9821..9889,9890..10681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10039..10058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga catgattacg aatttggtct ctgcaaaagt
     1021 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     1081 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     1141 gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc agacatgata
     1201 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1261 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1321 cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca
     1381 tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     1441 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     1501 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     1561 ccagagagac ccagtacaag caaaaagcag atcttatctt cgttgggagt gaattagccc
     1621 ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     1681 tggtcttaaa ggtaccaggc ggggaggcgg cccaaaggga gatccgactc gtctgagggc
     1741 gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg
     1801 ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa
     1861 cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag
     1921 tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg
     1981 tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc
     2041 cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa
     2101 cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga
     2161 aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg
     2221 taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg taatccagag
     2281 gttgattgtc gagtcgacgc ggccgctcag gcaccgggct tgcgggtcat gcaccaggtg
     2341 cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     2401 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc
     2461 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     2521 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     2581 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg
     2641 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     2701 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     2761 agctcggtga cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag
     2821 tcggcgaacg cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg
     2881 cgcaccgtgg gcttgtactc ggtcatgaat tcagggccgg gattctcctc cacgtcaccg
     2941 catgttagaa gacttcctct gccctctcga acagagagag accggccaga gggtttggcc
     3001 gcgcgcttga atttcgcgta ccgctctcgc gcaatttcga gtgtcgagtt cctgacacgc
     3061 tcaaagcgtt ctttgtcctt ctgccatagg ctacgaacag caagcccacg caccgaattg
     3121 aatatcaacc aaaggatatc ttctgcatca tcacgcgaaa gaccacggct caccagaaca
     3181 ccaagccaca tatcctcgac gacaaaccga ttcctctcta ccgtgcgctg aataccctcg
     3241 cgtaacgctg gatcccggtc ggcagccaca atcaaatcaa ggctgataga gaagtcatcg
     3301 tcgaggaaaa attcggcggc gtcgtccagc atttgctgga tgacgtcatc ctctggcttc
     3361 aatttcgcta atcgagcccg actgcgttcg gtgatctgtt cgtaaagcca ttcaaaagtg
     3421 gcaagcagaa gctcaagctt tgtcgggaaa tgatggctct gcgctcctct cgagacacct
     3481 gcagcaccgg gcacatctgc gatccggaat cccgcgtaac ctttttcccg taaaaccccc
     3541 agggccgctg caatcaactt gccctgggtc tccattgcgc gctctgcctg tgttcttctc
     3601 tttggactca tgatcacggg tctgggtctc tttggcatgg tagatcttcc aaacttggac
     3661 ctgggagtgg acacctgtgg agagaaaggc aaagtggatg tcatcggtct ctgctgcctc
     3721 accaaactag cgtaatctgg aacatcgtat gggtaagcca tggcggctct agagtaggcg
     3781 ccggtcacag cttggatctg taacggcgca gaacagaaaa cgaaacaaag acgtagagtt
     3841 gagcaagcag ggtcaggcaa agcgtggaga gccggctgag tctaggtagg ctccaaggga
     3901 gcgccggaca aaggcccggt ctcgacctga gctttaaact tacctagacg gcggacgcag
     3961 ttcaggaggc accacaggcg ggaggcggca gaacgcgact caaccggcgt ggatggcggc
     4021 ctcaggtagg gcggcgggcg cgtgaaggag agatgcgagc ccctcgaagc ttcagctgtg
     4081 ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg
     4141 gttctccccc accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta
     4201 ccccgcgcca ccttctctag gcacccgttc aattgccgac ccctcccccc aacttctcgg
     4261 ggactgtggg cgatgtgcgc tctgcccact gacgggcacc ggagcgatcg cagatccgcg
     4321 gccgcggatc cgatttaaat tcgaattagc tccctaaccc agcccaggct tgcccagtct
     4381 gtggccctcg tgggccagca gccactcttt cacggcgagc ccgccctcgt agccccccac
     4441 gtccaggtcg ccctgcacca cccggtggca ggggatcaga atgggcacgg gatttccgct
     4501 cagggcggtt ttcacggcgg cggtggcggc gggattgccg gccagggcgg ccaggtggct
     4561 gtagctgatg acctctccga acttcaccac tttcagcagt ttccacagca cctggcgggt
     4621 aaagctctcc tgctggaaca ctgggtggtg cagggctggc acagggaact cctcgatggc
     4681 ctcaggctgg tgaaagtagg cgttgagcca ggcggtggcc tgcatcagtg gctctggtcc
     4741 gcccagcacg gcggctgggg caggcacttc cacggcgtcg gcggcagatg ttcctttgcc
     4801 caggaagatg atacggtgca ggccctgttc gcacccagac agttccagct tgcccagagg
     4861 gctatccagg gtggtgcgct tcatttcgca gtctttgtcc attcctccga attctcctcc
     4921 aaccttcctc ttcttcttag gaggcctcag gctgcttcgg gaaacctgtc gtgccagctg
     4981 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     5041 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     5101 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     5161 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     5221 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     5281 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     5341 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     5401 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     5461 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     5521 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     5581 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     5641 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     5701 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     5761 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     5821 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     5881 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     5941 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     6001 gtataacgtt actggtttca ccatggtggc gctagctcta gaccaaactt ggacctggga
     6061 gtggacacct gtggagagaa aggcaaagtg gatgtcattg tcactcaagt gtatggccag
     6121 atcgggccag gtgaatatca aatcctcctc gtttttggaa actgacaatc ttagcgcaga
     6181 agtaatgccc gcttttgaga gggagtactc accccaacag ctggatctca agcctgccac
     6241 acctcacctc gaccatccgc cgtctcaaga ccgcctactt taattacatc atcagcagca
     6301 cctccgccag aaacaacccc gaccgccacc cgctgccgcc cgccacggtg ctcagcctac
     6361 cttgcgactg tgactggtta gacgcctttc tcgagaggtt ttccgatccg gtcgatgcgg
     6421 actcgctcag gtccctcggt ggcggagtac cgttcggagg ccgacgggtt tccgatccaa
     6481 gagtactgga aagaccgcga agagtttgtc ctcaaccgcg agcccaacag ctggccctcg
     6541 cagacagcga tgcggaagag agtgaccgcg gaggctggat cggtcccggt gtcttctatg
     6601 gaggtcaaaa cagcgtggat ggcgtctcca ggcgatatga ggcgcgcccg gttcactaaa
     6661 caccggtaac aaacagacaa tctggtctgt ttgattatac cggttgctta tataggcctc
     6721 ccaccgtaca cgcctacctc gacccggcaa gcttgcccat ttgcgtcaac ggggcggggg
     6781 tattacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     6841 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattggtgt
     6901 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     6961 taggaaagtc ccgtaaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     7021 tgacgtcaat agggggcgga cttggcatat gatacacttg atgtactgcc aagtgggcag
     7081 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     7141 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     7201 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     7261 taattgatta ctattaataa ctagtctatc gataaaattt tgaattttgt aatttgtttt
     7321 tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg
     7381 taccccccaa tccccccttt tcttttaaaa gttaaccgat accgtcgaga tccgttcact
     7441 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
     7501 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     7561 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     7621 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     7681 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     7741 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     7801 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     7861 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     7921 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     7981 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     8041 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     8101 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     8161 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     8221 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     8281 aagatcagtg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     8341 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     8401 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     8461 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     8521 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     8581 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     8641 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     8701 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     8761 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     8821 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     8881 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     8941 ccagagagct cccaggctca gatctggtct aaccagagag acccgtttat gtatcgagct
     9001 aggcacttaa atacaatatc tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc
     9061 agacccgtct gttgccttcc taataaggca cgatcgtacc accttacttc caccaatcgg
     9121 catgcacggt gctttttctc tccttgtaag gcatgttgct aactcatcgt taccatgttg
     9181 caagactaca agagtattgc ataagactac acgcgtcagc ttggcactgg ccgtcgtttt
     9241 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     9301 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     9361 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     9421 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     9481 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     9541 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     9601 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     9661 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     9721 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9781 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9841 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9961 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    10021 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    10081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    10141 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    10201 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    10261 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    10321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    10381 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    10441 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    10501 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    10561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    10621 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    10681 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    10741 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10801 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
