LOCUS       sequence-450142-       10891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Condensate module, light-inducible oligomerization 'core'.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, 
            Brangwynne CP
  TITLE     Condensate interfacial forces reposition DNA loci and probe 
            chromatin viscoelasticity.
  JOURNAL   Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 
            10.1016/j.cell.2024.07.034.
  PUBMED    39168125
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034."
COMMENT     Sequence Label: NLS-GFP-iLID-Ferritin
FEATURES             Location/Qualifiers
     source          1..10891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2975..2994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3029..3048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4542..4775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4960..5004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5289..5406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5559..5966
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      6037..6046
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6112..6549
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             6580..7293
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6610..6638)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7233..7253
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    7994..8582
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8047..8067)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8465..8476)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8585..8601)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8586..8602)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8692..8925
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8950..8968)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8951..8968)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9258..9277)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9491..9510)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9610..9632
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9670..9688)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9756..9860
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9861..9929,9930..10721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10079..10098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gtgttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     4441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     4501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     4561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     5041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     5101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     5161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     5221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     5281 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     5341 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     5401 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     5461 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     5521 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     5581 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     5641 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     5701 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     5761 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     5821 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     5881 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     5941 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgtc
     6001 aagtggagca aggcaggtgg acagtggatc actagtgcca ccatggcgga gccgcgtagc
     6061 aaacgtccgc gtgtgacgct gccggcgtgt ccggcgcata gtggggagtt tctggcaacc
     6121 acactggaac ggatcgagaa aaatttcgtg attactgatc cgagactgcc tgacaaccca
     6181 atcatttttg cgagcgattc cttcctgcag ctgacagaat attctcggga agagatcctg
     6241 gggcgcaatt gccgttttct gcagggaccc gagacagacc gtgccactgt tcggaaaatc
     6301 agagatgcta ttgacaacca gactgaagtg accgttcagc tgatcaatta taccaagagc
     6361 ggcaagaagt tctggaacgt gttccacctg cagccgatgc gcgattataa gggcgacgtc
     6421 cagtacttca ttggcgtgca gctggatggc accgaacgtc ttcatggcgc cgctgagcgt
     6481 gaggcggtct gcctgatcaa aaagacagcc tttcagattg ctgaggcagc gaacgacgaa
     6541 aattactttg gcggctccgg ctctggatcc ggagctacca tgagtaaagg agaagaactt
     6601 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     6661 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     6721 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactaccct gacttatggt
     6781 gttcaatgct tttcaagata cccagatcat atgaaacggc atgacttttt caagagtgcc
     6841 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     6901 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     6961 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     7021 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     7081 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     7141 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctaagctt
     7201 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     7261 gggattacac atggcatgga tgaactatac aaaggtggat ctggaggttc aggtggaagt
     7321 gcggccggta ccggcagcgg aggaatgacg accgcgtcca cctcgcaggt gcgccagaac
     7381 taccaccagg actcagaggc cgccatcaac cgccagatca acctggagct ctacgcctcc
     7441 tacgtttacc tgtccatgtc ttactacttt gaccgcgatg atgtggcttt gaagaacttt
     7501 gccaaatact ttcttcacca atctcatgag gagagggaac atgctgagaa actgatgaag
     7561 ctgcagaacc aacgaggtgg ccgaatcttc cttcaggata tcaagaaacc agactgtgat
     7621 gactgggaga gcgggctgaa tgcaatggag tgtgcattac atttggaaaa aaatgtgaat
     7681 cagtcactac tggaactgca caaactggcc actgacaaaa atgaccccca tttgtgtgac
     7741 ttcattgaga cacattacct gaatgagcag gtgaaagcca tcaaagaatt gggtgaccac
     7801 gtgaccaact tgcgcaagat gggagcgccc gaatctggct tggcggaata tctctttgac
     7861 aagcacaccc tgggagacag tgataatgaa agctgagcgg ccgcaactcc cacctgcaac
     7921 atgcgtgact gactgaggcc gcgactctag agtcgacctg caggcatgca agcttgatat
     7981 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     8041 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8101 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     8161 ttatcaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     8221 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     8281 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     8341 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     8401 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     8461 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     8521 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     8581 gcatcgatac cgtcgacctc gagggaatta attcgagctc ggtaccttta agaccaatga
     8641 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8701 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8761 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8821 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8881 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat
     8941 tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
     9001 attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact
     9061 gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac
     9121 cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag
     9181 ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     9241 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     9301 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     9361 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     9421 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt
     9481 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     9541 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     9601 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     9661 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     9721 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9841 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9901 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9961 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    10021 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10081 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    10141 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10201 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10261 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10321 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10381 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10441 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10501 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10561 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10621 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10681 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10741 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10801 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10861 cagaccccgt agaaaagatc aaaggatctt c
//
