LOCUS       sequence-450144-       12014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adhesion module, creates 'seed' at telomere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, 
            Brangwynne CP
  TITLE     Condensate interfacial forces reposition DNA loci and probe 
            chromatin viscoelasticity.
  JOURNAL   Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 
            10.1016/j.cell.2024.07.034.
  PUBMED    39168125
REFERENCE   2  (bases 1 to 12014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034."
COMMENT     Sequence Label: FUS-5YS-miRFP670-TRF1
FEATURES             Location/Qualifiers
     source          1..12014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2975..2994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3029..3048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4542..4775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4960..5004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5289..5406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5559..5966
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6794..7738
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    9117..9705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9170..9190)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9588..9599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9708..9724)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9709..9725)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9815..10048
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10073..10091)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10074..10091)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10381..10400)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10614..10633)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10733..10755
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10793..10811)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10879..10983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10984..11052,11053..11844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11202..11221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     4441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     4501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     4561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     5041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     5101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     5161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     5221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     5281 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     5341 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     5401 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     5461 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     5521 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     5581 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     5641 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     5701 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     5761 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     5821 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     5881 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     5941 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgtc
     6001 aagtggagca aggcaggtgg acagtggatc actagtcgga gctaccatgg cctcaaacga
     6061 ttatacccaa caagcaaccc aaagctatgg ggcctccccc acccagcccg ggcagggcta
     6121 ttcccagcag agcagtcagc cctacggaca gcagagttac agtggttata gccagtccac
     6181 ggacacttca ggctatggcc agagcagcta ttcttcttat ggccagagcc agaacacagg
     6241 ctatggaact cagtcaactc cccagggatc tggctcgact ggcggctctg gcagtagcca
     6301 gagctcccaa tcgtcttacg ggcagcagtc ctcctatcct ggctatggcc agcagccagc
     6361 tcccagcagc acctcgggaa gttacggtag cagttctcag agcagcagct atgggcagcc
     6421 ccagagtgga agctacagcc agcagcctag ttatggtgga cagcagcaaa gctctggaca
     6481 gcagcaaagc tctaatcccc ctcagggcta tggacagcag aaccagtaca acagcagcag
     6541 tggtggtgga ggtggaggtg gaggtggagg taactatggc caagatcaat cctccatgag
     6601 tagtggtggt ggcagtggtg gcggttatgg caatcaagac cagagtggtg gaggtggcag
     6661 cggtggctat ggacagcagg accgtggagg atctggctcc ggttctgctt caggatccgg
     6721 atcaggtatg gtagcaggtc atgccggatc cggatcaggt atggtagcag gtcatgccgg
     6781 atccggatca ggtatggtag caggtcatgc ctctggcagc cccgcattcg ggaccgcctc
     6841 tcattcgaat tgcgaacatg aagagatcca cctcgccggc tcgatccagc cgcatggcgc
     6901 gcttctggtc gtcagcgaac atgatcatcg cgtcatccag gccagcgcca acgccgcgga
     6961 atttctgaat ctcggaagcg tactcggcgt tccgctcgcc gagatcgacg gcgatctgtt
     7021 gatcaagatc ctgccgcatc tcgatcccac cgccgaaggc atgccggtcg cggtgcgctg
     7081 ccggatcggc aatccctcta cggagtactg cggtctgatg catcggcctc cggaaggcgg
     7141 gctgatcatc gaactcgaac gtgccggccc gtcgatcgat ctgtcaggca cgctggcgcc
     7201 ggcgctggag cggatccgca cggcgggttc actgcgcgcg ctgtgcgatg acaccgtgct
     7261 gctgtttcag cagtgcaccg gctacgaccg ggtgatggtg tatcgtttcg atgagcaagg
     7321 ccacggcctg gtattctccg agtgccatgt gcctgggctc gaatcctatt tcggcaaccg
     7381 ctatccgtcg tcgactgtcc cgcagatggc gcggcagctg tacgtgcggc agcgcgtccg
     7441 cgtgctggtc gacgtcacct atcagccggt gccgctggag ccgcggctgt cgccgctgac
     7501 cgggcgcgat ctcgacatgt cgggctgctt cctgcgctcg atgtcgccgt gccatctgca
     7561 gttcctgaag gacatgggcg tgcgcgccac cctggcggtg tcgctggtgg tcggcggcaa
     7621 gctgtggggc ctggttgtct gtcaccatta tctgccgcgc ttcatccgtt tcgagctgcg
     7681 ggcgatctgc aaacggctcg ccgaaaggat cgcgacgcgg atcaccgcgc ttgagagctc
     7741 cggaggcggc ggatccggcg gatctggaat ggcggaggat gtttcctcag cggccccgag
     7801 cccgcggggc tgtgcggatg gtagggatgc cgaccctact gaggagcaga tggcagaaac
     7861 agagagaaac gacgaggagc agttcgaatg ccaggaactg ctcgagtgcc aggtgcaggt
     7921 gggggccccc gaggaggagg aggaggagga ggaggacgcg ggcctggtgg ccgaggccga
     7981 ggccgtggct gccggctgga tgctcgattt cctctgcctc tctctttgcc gagctttccg
     8041 cgacggccgc tccgaggact tccgcaggac ccgcaacagc gcagaggcta ttattcatgg
     8101 actatccagt ctaacagctt gccagttgag aacgatatac atatgtcagt ttttgacaag
     8161 aattgcagca ggaaaaaccc ttgatgcaca gtttgaaaat gatgaacgaa ttacaccctt
     8221 ggaatcagcc ctgatgattt ggggttcaat tgaaaaggaa catgacaaac ttcatgaaga
     8281 aatacagaat ttaattaaaa ttcaggctat agctgtttgt atggaaaatg gcaactttaa
     8341 agaagcagaa gaagtctttg aaagaatatt tggtgatcca aattctcata tgcctttcaa
     8401 aagcaaattg cttatgataa tctctcagaa agatacattt cattcctttt ttcaacactt
     8461 cagctacaac cacatgatgg agaaaattaa gagttatgtg aattatgtgc taagtgaaaa
     8521 atcatcaacc tttctaatga aggcagcggc aaaagtagta gaaagcaaaa ggacaagaac
     8581 aataacttct caagataaac ctagtggtaa tgatgttgaa atggaaactg aagctaattt
     8641 ggatacaaga aaaagtgtta gtgacaaaca gtctgcggta actgaatcct cagagggtac
     8701 agtatcctta ttgaggtctc acaagaatct tttcttatct aagttgcaac atggaaccca
     8761 gcaacaagac cttaataaga aagaaagaag agtaggaact cctcaaagta caaaaaagaa
     8821 aaaagaaagc agaagagcca ctgaaagcag aatacctgtt tcaaagagtc agccggtaac
     8881 tcctgaaaaa catcgagcta gaaaaagaca ggcatggctt tgggaagaag acaagaattt
     8941 gagatctggc gtgaggaaat atggagaggg aaactggtct aaaatactgt tgcattataa
     9001 attcaacaac cggacaagtg tcatgttaaa agacagatgg aggaccatga agaaactaaa
     9061 actgatttcc tcagacagcg aagacggcat gcaagcttga tatcaagctt atcgataatc
     9121 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     9181 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     9241 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     9301 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     9361 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     9421 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     9481 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     9541 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     9601 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     9661 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     9721 ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     9781 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     9841 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     9901 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9961 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10021 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
    10081 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
    10141 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
    10201 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
    10261 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
    10321 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
    10381 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
    10441 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
    10501 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
    10561 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
    10621 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
    10681 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    10741 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
    10801 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    10861 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10921 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10981 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    11041 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    11101 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    11161 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    11221 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    11281 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    11341 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    11401 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    11461 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    11521 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    11581 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg aacacttctg
    11641 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    11701 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    11761 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    11821 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    11881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    11941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    12001 atcaaaggat cttc
//
