LOCUS       sequence-450145-        6038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Condensate module, light-inducible oligomerization IDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, 
            Brangwynne CP
  TITLE     Condensate interfacial forces reposition DNA loci and probe 
            chromatin viscoelasticity.
  JOURNAL   Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 
            10.1016/j.cell.2024.07.034.
  PUBMED    39168125
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034."
COMMENT     Sequence Label: FUS-mCh-sspB
FEATURES             Location/Qualifiers
     source          1..6038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             1696..1707
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2705..3034
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     misc_feature    3141..3729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3194..3214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3612..3623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3732..3748)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3733..3749)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3839..4072
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4097..4115)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4098..4115)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4405..4424)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4638..4657)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4757..4779
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4817..4835)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4903..5007
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5008..5076,5077..5868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5226..5245)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaatgaaa atagctaacg ttgggccaaa caggatatct gcggtgagca gtttcggccc
      961 cggcccgggg ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagaac
     1021 agatggtccc cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc
     1081 aggctccccc aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc
     1141 ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct
     1201 cactcggcgc gccagtcctc cgacagactg agtcgcccgg gggggatctg gagctctcga
     1261 gaattctcac gcgtcaagtg gagcaaggca ggtggacagt ggatccggag ctaccatggc
     1321 ctcaaacgat tatacccaac aagcaaccca aagctatggg gcctacccca cccagcccgg
     1381 gcagggctat tcccagcaga gcagtcagcc ctacggacag cagagttaca gtggttatag
     1441 ccagtccacg gacacttcag gctatggcca gagcagctat tcttcttatg gccagagcca
     1501 gaacacaggc tatggaactc agtcaactcc ccagggatat ggctcgactg gcggctatgg
     1561 cagtagccag agctcccaat cgtcttacgg gcagcagtcc tcctatcctg gctatggcca
     1621 gcagccagct cccagcagca cctcgggaag ttacggtagc agttctcaga gcagcagcta
     1681 tgggcagccc cagagtggga gctacagcca gcagcctagc tatggtggac agcagcaaag
     1741 ctatggacag cagcaaagct ataatccccc tcagggctat ggacagcaga accagtacaa
     1801 cagcagcagt ggtggtggag gtggaggtgg aggtggaggt aactatggcc aagatcaatc
     1861 ctccatgagt agtggtggtg gcagtggtgg cggttatggc aatcaagacc agagtggtgg
     1921 aggtggcagc ggtggctatg gacagcagga ccgtggaggt ggaggaatgg tgtctaaagg
     1981 cgaggaggat aacatggcta taatcaagga atttatgagg ttcaaggtgc atatggaggg
     2041 atctgtgaac ggtcacgagt tcgaaatcga gggcgaaggc gaggggcgac cctatgaagg
     2101 gacacagacg gccaaactga aggtgaccaa gggtggcccc ctgcctttcg cctgggacat
     2161 cctgtcaccc cagtttatgt acggctctaa ggcttacgtt aagcaccctg ccgatattcc
     2221 ggactacttg aaactgagct tccctgaagg gttcaagtgg gaacgggtaa tgaattttga
     2281 ggatggcggt gtagtcacag ttacccagga cagctctctt caggacggag aatttatcta
     2341 taaggttaaa cttcggggca ctaacttccc atcagacggc cccgtgatgc agaagaaaac
     2401 tatgggctgg gaagccagct cagaacgcat gtatcccgag gacggagcac tgaagggaga
     2461 aatcaagcag cgactcaagc tgaaggatgg gggacattat gatgctgagg tcaaaaccac
     2521 ctataaggcc aagaaacccg ttcagcttcc tggggcatac aacgtgaata tcaaactgga
     2581 tattacatcc cataacgaag actataccat cgtggaacag tacgagcggg ccgagggcag
     2641 acatagcaca gggggcatgg atgaattgta caaggcggcc ggatccggtg gtacctctgg
     2701 aggtagctcc ccgaaacgcc ctaagctgct gcgtgaatat tacgattggc tggttgataa
     2761 cagctttacc ccatatctgg tggtggatgc cacatacctg ggcgtgaacg tgcccgtgga
     2821 gtatgtgaaa gacggtcaga tcgtgctgaa tctgtctgca agtgcgaccg gcaacctgca
     2881 actgacaaat gattttatcc agttcaacgc ccgctttaag ggcgtgtctc gtgaactgta
     2941 tatcccgatg ggtgccgctc tggccattta cgctcgcgag aacggcgatg gtgtgatgtt
     3001 cgaaccagaa gaaatctatg acgagctgaa tattggttaa ggatcggccg caactcccac
     3061 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcag gcatgcaagc
     3121 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3541 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3661 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3721 cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga
     3781 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     3841 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     3901 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3961 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4021 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     4081 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     4141 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     4201 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     4261 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     4321 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     4381 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     4441 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     4501 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     4561 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     4621 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     4681 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4741 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4801 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4861 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4921 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4981 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5041 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5101 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5161 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5221 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5281 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5341 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5401 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5461 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5521 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5581 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5641 gataaagttg caggaacact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5701 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5761 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5821 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5881 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5941 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6001 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
