LOCUS       sequence-450146-       11057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a reporter for a mitochondrial matrix protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Muthukumar G, Stevens TA, Inglis AJ, Esantsi TK, Saunders RA, 
            Schulte F, Voorhees RM, Guna A, Weissman JS
  TITLE     Triaging of alpha-helical proteins to the mitochondrial outer 
            membrane by distinct chaperone machinery based on substrate 
            topology.
  JOURNAL   Mol Cell. 2024 Mar 21;84(6):1101-1119.e9. doi: 
            10.1016/j.molcel.2024.01.028. Epub 2024 Feb 29.
  PUBMED    38428433
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.01.028"; journalName: "Mol Cell"; date: 
            "2024-03-21- 21"; volume: "84"; issue: "6"; pages: "1101-1119.e9"
COMMENT     Sequence Label: pAG27
FEATURES             Location/Qualifiers
     source          1..11057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2047..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2896..4073
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4129..4194
                     /codon_start=1
                     /product="mitochondrial presequence of yeast cytochrome c 
                     oxidase subunit IV (Hurt et al., 1984)"
                     /label=pre-CoxIV
                     /translation="MLSLRQSIRFFKPATRTLCSSR"
     misc_feature    4918..5506
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4971..4991)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5389..5400)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5509..5525)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5510..5526)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5616..5849
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5874..5892)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5875..5892)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6182..6201)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6415..6434)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6534..6556
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6594..6612)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6680..6784
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6785..6853,6854..7645)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7003..7022)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7816..8404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8305..8324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8558..8575
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8645..8665)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8650..8979
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8830..8965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10113..10178
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10308..10328
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10753..10887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10790..10809)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10844..10863
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11055..11057,1..631)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
       61 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1141 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     2041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2161 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     2221 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
     2281 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
     2341 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
     2401 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
     2461 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
     2521 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     2581 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     2641 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     2701 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc
     2761 cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc
     2821 ctccccccgg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     2881 ctgtaggcgc ggaggggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaattct
     4081 cacgcgtctg caggatatca agcttgcggt accgcgggcc cggccaccat gctttcacta
     4141 cgtcaatcta taagattttt caagccagcc acaagaactt tgtgtagctc tagacgggat
     4201 ccaccggtca tgagcaaagg cgaggagctg ttcacaggcg tggtgccaat cctggtggag
     4261 ctggacggcg acgtgaacgg ccacaaattc agcgtgagag gcgagggcga gggcgacgcc
     4321 acaatcggca aactgacact gaaattcatc tgcacaacag gcaaactgcc agtgccctgg
     4381 ccaacactag tgacaacact gacatacggc gtgcagtgct tcagcagata tccggaccac
     4441 atgaaaagac acgacttctt caaaagcgcc atgccagagg gctacgtgca ggagagaaca
     4501 atcagcttca aagacgacgg caaatacaaa acaagagccg tggtgaaatt cgagggcgac
     4561 acactggtga acagaatcga gctgaaaggc acagacttca aagaggacgg caacatcctg
     4621 ggccacaaac tggagtacaa cttcaacagc cacaacgtgt acatcacagc caacaaacag
     4681 aaaaacggca tcaaagccaa cttcacagtg agacacaacg tggaggacgg cagcgtgcag
     4741 ctcgccgacc actaccagca gaacacacca atcggcgacg gcccagtgct gctgccagac
     4801 aaccactacc tgagcacaca gacagtgctg agcaaagacc caaacgagaa ataataataa
     4861 ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct tatcgataat
     4921 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4981 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5041 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5101 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5161 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5221 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5281 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     5341 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5401 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5461 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     5521 cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     5581 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5641 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5701 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5761 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5821 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     5881 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     5941 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     6001 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     6061 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     6121 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     6181 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     6241 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     6301 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     6361 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     6421 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     6481 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6541 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     6601 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6661 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6721 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6781 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6841 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6901 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6961 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7021 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7081 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7141 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7201 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7261 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7321 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7381 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7441 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7501 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7561 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7621 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7681 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7921 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8221 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8461 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8521 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8581 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8641 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8701 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8761 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8821 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8881 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8941 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     9001 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     9061 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     9121 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     9181 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     9241 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     9301 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     9361 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     9421 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     9481 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     9541 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     9601 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     9661 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     9721 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     9781 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     9841 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     9901 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     9961 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
    10021 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
    10081 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
    10141 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
    10201 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
    10261 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
    10321 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
    10381 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
    10441 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
    10501 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
    10561 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
    10621 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
    10681 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    10741 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10801 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10861 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10921 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    10981 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    11041 acaaacttag tagttgg
//
