LOCUS       sequence-450152-       11156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a reporter for a mitochondrial intermembrane space 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Guna A, Stevens TA, Inglis AJ, Replogle JM, Esantsi TK, Muthukumar 
            G, Shaffer KCL, Wang ML, Pogson AN, Jones JJ, Lomenick B, Chou TF, 
            Weissman JS, Voorhees RM
  TITLE     MTCH2 is a mitochondrial outer membrane protein insertase.
  JOURNAL   Science. 2022 Oct 21;378(6617):317-322. doi: 
            10.1126/science.add1856. Epub 2022 Oct 20.
  PUBMED    36264797
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add1856"; journalName: "Science"; date: 
            "2022-10-21- 21"; volume: "378"; issue: "6617"; pages: "317-322"
COMMENT     Sequence Label: pAG26
FEATURES             Location/Qualifiers
     source          1..11156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2047..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2896..4073
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5017..5605
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5070..5090)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5488..5499)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5608..5624)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5609..5625)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5715..5948
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5973..5991)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5974..5991)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6281..6300)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6514..6533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6633..6655
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6693..6711)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6779..6883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6884..6952,6953..7744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7102..7121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7915..8503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8404..8423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8657..8674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8744..8764)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8749..9078
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8929..9064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10212..10277
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10407..10427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10852..10986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10889..10908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10943..10962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11154..11156,1..631)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
       61 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1141 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     2041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2161 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     2221 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
     2281 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
     2341 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
     2401 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
     2461 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
     2521 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     2581 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     2641 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     2701 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc
     2761 cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc
     2821 ctccccccgg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     2881 ctgtaggcgc ggaggggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaattct
     4081 cacgcgtctg caggatatca agcttgcggt accgcgggcc cggccaccat gtttcgtctg
     4141 aactcacttt ctgctttggc agaactggct gtgggttctc gatggtacca tggaggatca
     4201 cagcccatcc agatccggcg aagactaatg atggtggctt tcctgggagc atctgcagta
     4261 actgcaagta ctggtctttt gtggaagagg gcccatgcag aatctccaat gagcaaaggc
     4321 gaggagctgt tcacaggcgt ggtgccaatc ctggtggagc tggacggcga cgtgaacggc
     4381 cacaaattca gcgtgagagg cgagggcgag ggcgacgcca caatcggcaa actgacactg
     4441 aaattcatct gcacaacagg caaactgcca gtgccctggc caacactagt gacaacactg
     4501 acatacggcg tgcagtgctt cagcagatat ccggaccaca tgaaaagaca cgacttcttc
     4561 aaaagcgcca tgccagaggg ctacgtgcag gagagaacaa tcagcttcaa agacgacggc
     4621 aaatacaaaa caagagccgt ggtgaaattc gagggcgaca cactggtgaa cagaatcgag
     4681 ctgaaaggca cagacttcaa agaggacggc aacatcctgg gccacaaact ggagtacaac
     4741 ttcaacagcc acaacgtgta catcacagcc aacaaacaga aaaacggcat caaagccaac
     4801 ttcacagtga gacacaacgt ggaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4861 aacacaccaa tcggcgacgg cccagtgctg ctgccagaca accactacct gagcacacag
     4921 acagtgctga gcaaagaccc aaacgagaaa taataataag gccgcgactc tagagtcgac
     4981 ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     5041 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5101 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5161 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5221 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5281 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5341 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5401 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     5461 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5521 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5581 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa ttaattcgag
     5641 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5701 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     5761 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5821 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5881 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5941 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     6001 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     6061 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     6121 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     6181 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     6241 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     6301 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     6361 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     6421 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     6481 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6541 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6601 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6661 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     6721 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6781 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6841 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6901 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6961 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7021 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7081 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7141 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7201 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7261 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7321 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7381 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7441 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7501 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7561 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7621 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7681 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7741 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7801 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7861 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7921 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7981 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8041 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8101 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8161 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8221 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8281 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8341 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8401 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8461 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8521 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8581 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8641 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8701 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
     8761 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8821 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8881 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8941 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9001 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9061 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
     9121 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     9181 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     9241 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     9301 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     9361 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt
     9421 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     9481 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
     9541 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
     9601 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     9661 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     9721 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
     9781 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     9841 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     9901 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
     9961 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga
    10021 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
    10081 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct
    10141 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
    10201 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
    10261 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
    10321 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
    10381 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
    10441 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
    10501 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
    10561 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
    10621 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
    10681 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
    10741 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
    10801 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    10861 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10921 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10981 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    11041 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    11101 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttgg
//
