LOCUS       sequence-450156-       11900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adhesion module, creates 'seed' at telomere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11900)
  AUTHORS   Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, 
            Brangwynne CP
  TITLE     Condensate interfacial forces reposition DNA loci and probe 
            chromatin viscoelasticity.
  JOURNAL   Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 
            10.1016/j.cell.2024.07.034.
  PUBMED    39168125
REFERENCE   2  (bases 1 to 11900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034."
COMMENT     Sequence Label: FUS-27YS-miRFP670-TRF1
FEATURES             Location/Qualifiers
     source          1..11900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2975..2994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3029..3048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4542..4775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4960..5004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5289..5406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5559..5966
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6680..7624
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    9003..9591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9056..9076)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9474..9485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9594..9610)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9595..9611)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9701..9934
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9959..9977)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9960..9977)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10267..10286)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10500..10519)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10619..10641
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10679..10697)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10765..10869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10870..10938,10939..11730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11088..11107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     4441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     4501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     4561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     5041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     5101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     5161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     5221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     5281 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     5341 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     5401 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     5461 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     5521 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     5581 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     5641 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     5701 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     5761 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     5821 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     5881 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     5941 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgat
     6001 ggcctcaaac gattctaccc aacaagcaac ccaaagctct ggggcctccc ccacccagcc
     6061 cgggcagggc tcttcccagc agagcagtca gccctccgga cagcagagtt ccagtggttc
     6121 tagccagtcc acggacactt caggctctgg ccagagcagc tcttcttctt ctggccagag
     6181 ccagaacaca ggctctggaa ctcagtcaac tccccaggga tctggctcga ctggcggctc
     6241 tggcagtagc cagagctccc aatcgtcttc cgggcagcag tcctcctctc ctggctctgg
     6301 ccagcagcca gctcccagca gcacctcggg aagttccggt agcagttctc agagcagcag
     6361 ctctgggcag ccccagagtg gaagctccag ccagcagcct agttctggtg gacagcagca
     6421 aagctctgga cagcagcaaa gctctaatcc ccctcagggc tctggtcagc agaaccagtc
     6481 caacagcagc agtggtggtg gaggtggagg tggaggtgga ggtaactctg gccaagatca
     6541 atcctccatg agtagtggtg gtggcagtgg tggcggttct ggcaatcaag accagagtgg
     6601 tggaggtggc agcggtggct ctggacagca ggaccgtgga ggatctggct ccggttctgc
     6661 ttcaggatcc ggatcaggta tggtagcagg tcatgcctct ggcagccccg cattcgggac
     6721 cgcctctcat tcgaattgcg aacatgaaga gatccacctc gccggctcga tccagccgca
     6781 tggcgcgctt ctggtcgtca gcgaacatga tcatcgcgtc atccaggcca gcgccaacgc
     6841 cgcggaattt ctgaatctcg gaagcgtact cggcgttccg ctcgccgaga tcgacggcga
     6901 tctgttgatc aagatcctgc cgcatctcga tcccaccgcc gaaggcatgc cggtcgcggt
     6961 gcgctgccgg atcggcaatc cctctacgga gtactgcggt ctgatgcatc ggcctccgga
     7021 aggcgggctg atcatcgaac tcgaacgtgc cggcccgtcg atcgatctgt caggcacgct
     7081 ggcgccggcg ctggagcgga tccgcacggc gggttcactg cgcgcgctgt gcgatgacac
     7141 cgtgctgctg tttcagcagt gcaccggcta cgaccgggtg atggtgtatc gtttcgatga
     7201 gcaaggccac ggcctggtat tctccgagtg ccatgtgcct gggctcgaat cctatttcgg
     7261 caaccgctat ccgtcgtcga ctgtcccgca gatggcgcgg cagctgtacg tgcggcagcg
     7321 cgtccgcgtg ctggtcgacg tcacctatca gccggtgccg ctggagccgc ggctgtcgcc
     7381 gctgaccggg cgcgatctcg acatgtcggg ctgcttcctg cgctcgatgt cgccgtgcca
     7441 tctgcagttc ctgaaggaca tgggcgtgcg cgccaccctg gcggtgtcgc tggtggtcgg
     7501 cggcaagctg tggggcctgg ttgtctgtca ccattatctg ccgcgcttca tccgtttcga
     7561 gctgcgggcg atctgcaaac ggctcgccga aaggatcgcg acgcggatca ccgcgcttga
     7621 gagctccgga ggcggcggat ccggcggatc tggaatggcg gaggatgttt cctcagcggc
     7681 cccgagcccg cggggctgtg cggatggtag ggatgccgac cctactgagg agcagatggc
     7741 agaaacagag agaaacgacg aggagcagtt cgaatgccag gaactgctcg agtgccaggt
     7801 gcaggtgggg gcccccgagg aggaggagga ggaggaggag gacgcgggcc tggtggccga
     7861 ggccgaggcc gtggctgccg gctggatgct cgatttcctc tgcctctctc tttgccgagc
     7921 tttccgcgac ggccgctccg aggacttccg caggacccgc aacagcgcag aggctattat
     7981 tcatggacta tccagtctaa cagcttgcca gttgagaacg atatacatat gtcagttttt
     8041 gacaagaatt gcagcaggaa aaacccttga tgcacagttt gaaaatgatg aacgaattac
     8101 acccttggaa tcagccctga tgatttgggg ttcaattgaa aaggaacatg acaaacttca
     8161 tgaagaaata cagaatttaa ttaaaattca ggctatagct gtttgtatgg aaaatggcaa
     8221 ctttaaagaa gcagaagaag tctttgaaag aatatttggt gatccaaatt ctcatatgcc
     8281 tttcaaaagc aaattgctta tgataatctc tcagaaagat acatttcatt ccttttttca
     8341 acacttcagc tacaaccaca tgatggagaa aattaagagt tatgtgaatt atgtgctaag
     8401 tgaaaaatca tcaacctttc taatgaaggc agcggcaaaa gtagtagaaa gcaaaaggac
     8461 aagaacaata acttctcaag ataaacctag tggtaatgat gttgaaatgg aaactgaagc
     8521 taatttggat acaagaaaaa gtgttagtga caaacagtct gcggtaactg aatcctcaga
     8581 gggtacagta tccttattga ggtctcacaa gaatcttttc ttatctaagt tgcaacatgg
     8641 aacccagcaa caagacctta ataagaaaga aagaagagta ggaactcctc aaagtacaaa
     8701 aaagaaaaaa gaaagcagaa gagccactga aagcagaata cctgtttcaa agagtcagcc
     8761 ggtaactcct gaaaaacatc gagctagaaa aagacaggca tggctttggg aagaagacaa
     8821 gaatttgaga tctggcgtga ggaaatatgg agagggaaac tggtctaaaa tactgttgca
     8881 ttataaattc aacaaccgga caagtgtcat gttaaaagac agatggagga ccatgaagaa
     8941 actaaaactg atttcctcag acagcgaaga cggcatgcaa gcttgatatc aagcttatcg
     9001 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9061 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9121 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9181 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9241 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9301 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9361 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     9421 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     9481 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     9541 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     9601 gtcgacctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     9661 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9721 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9781 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9841 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9901 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     9961 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
    10021 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
    10081 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
    10141 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
    10201 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
    10261 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
    10321 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
    10381 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
    10441 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
    10501 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
    10561 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    10621 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
    10681 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
    10741 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    10801 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    10861 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    10921 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    10981 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    11041 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    11101 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    11161 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    11221 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    11281 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    11341 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    11401 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    11461 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggaaca
    11521 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    11581 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    11641 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    11701 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    11761 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    11821 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    11881 gaaaagatca aaggatcttc
//
