LOCUS       sequence-450157-       12758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a reporter for an alpha-helical OMM protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12758)
  AUTHORS   Guna A, Stevens TA, Inglis AJ, Replogle JM, Esantsi TK, Muthukumar 
            G, Shaffer KCL, Wang ML, Pogson AN, Jones JJ, Lomenick B, Chou TF, 
            Weissman JS, Voorhees RM
  TITLE     MTCH2 is a mitochondrial outer membrane protein insertase.
  JOURNAL   Science. 2022 Oct 21;378(6617):317-322. doi: 
            10.1126/science.add1856. Epub 2022 Oct 20.
  PUBMED    36264797
REFERENCE   2  (bases 1 to 12758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add1856"; journalName: "Science"; date: 
            "2022-10-21- 21"; volume: "378"; issue: "6617"; pages: "317-322"
COMMENT     Sequence Label: pAG391
FEATURES             Location/Qualifiers
     source          1..12758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2047..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2896..4073
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4796..4852
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6488..6535
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    6619..7207
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6672..6692)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7090..7101)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7210..7226)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7211..7227)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7317..7550
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7575..7593)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7576..7593)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7883..7902)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8116..8135)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8235..8257
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8295..8313)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8381..8485
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8486..8554,8555..9346)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8704..8723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9517..10105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10006..10025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10259..10276
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10346..10366)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10351..10680
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10531..10666
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11814..11879
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12009..12029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12454..12588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12491..12510)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12545..12564
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12756..12758,1..631)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
       61 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1141 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     2041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2161 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     2221 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
     2281 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
     2341 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
     2401 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
     2461 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
     2521 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     2581 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     2641 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     2701 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc
     2761 cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc
     2821 ctccccccgg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     2881 ctgtaggcgc ggaggggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaattct
     4081 cacgcgtgac tctagcgttt aaacttaagc ttacggtacc atgaacatgg cgattatcaa
     4141 ggaattcatg aggttcaaag ttcacatgga gggatctgta aacggccacg agttcgaaat
     4201 cgagggagag ggggagggtc gaccatatga aggaacacag accgctaaat tgaaggttac
     4261 taaaggtgga ccacttcctt tcgcatggga cattcttagc ccccagttta tgtatggttc
     4321 caaggcgtat gtcaaacatc ccgcagacat tcctgattac cttaaactga gttttccaga
     4381 ggggttcaaa tgggagcgcg tcatgaactt tgaagatggg ggcgttgtta cagtgactca
     4441 ggactcttct ttgcaagatg gagagtttat atataaggtg aaacttcgcg gaaccaactt
     4501 cccaagcgat ggcccagtca tgcagaagaa aactatgggt tgggaagcca gtagcgagcg
     4561 gatgtaccca gaggacggcg cacttaaggg tgagattaag cagcggctga aactcaaaga
     4621 cggaggccat tatgacgcgg aagttaaaac gacgtacaaa gctaaaaagc ctgtccaatt
     4681 gccaggcgcg tacaatgtca atattaaatt ggacattacc agtcacaacg aagattacac
     4741 aatagtggag caatacgaac gcgcagaggg acgccattca acagggggaa gcggagctac
     4801 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctggatcccc
     4861 gtttgctgaa gacaagacct ataagtatat ctgccgcaat ttcagcaatt tttgcaatgt
     4921 ggatgttgta gagattctgc cttacctgcc ctgcctcaca gcaagagacc aggatcgact
     4981 gcgggccacc tgcacactct cagggaaccg ggacaccctc tggcatctct tcaataccct
     5041 tcagcggcgg ccaggctggg tggaatactt cattgcggca ctgaggggct gtgagctagt
     5101 tgatctcgcg gacgaagtgg cctctgtcta cgaaagctac cagcctcgga catcggaccg
     5161 tcccccagac ccactggagc caccgtcact tcctgctgag aggccagggc cccctacacc
     5221 tgctgctgcc cacagcatcc cctacaacag ctgcagagaa aaggagccaa gttaccccat
     5281 gcctgtccag gaaacccagg cgccagagag cccaggagaa aattcagagc aagccctgca
     5341 gacgctcagc ccaagagcca tcccaaggaa tccagatggt ggccccctgg agtccagttc
     5401 tgacctggca gccctcagcc ctctgacctc cagcgggcat caggagaaag acacagaact
     5461 gggcagtact cacacagcag gtgcgacctc cagccttaca ccatcccgtg ggcctgtgtc
     5521 tccatctgta tccttccagc ccctggcccg ttccaccccc agggcaagcc gcttgcctgg
     5581 acccacagga tcagttgtat ctactggaac ctccttctca tcctcatccc ctggcttggc
     5641 ctctgcaggg gctgcagaag gtaaacaggg tgcagaaagt gaccaggccg agcctatcat
     5701 ctgctccagt ggagcagaag cacctgccaa ctctttgcca tctaaagtgc ctaccacctt
     5761 gatgcctgtg aacacagtgg ccctgaaagt ccctgccaat ccagcatctg tcagcacagt
     5821 gccctctaaa ttgccaacaa gttcaaaacc acctggtgca gtgccttcta atgcgctcac
     5881 caatccagca ccatccaaat tgccaatcaa ctcaacccgt gctggcatgg tgccatccaa
     5941 agtgcctact agcatggtgc tcaccaaggt gtctgccagc acagtcccca ctgacgggag
     6001 cagcagaaat gaggagaccc cagcagctcc aacacccgcc ggcgccactg gaggcagctc
     6061 agcctggcta gacagcagct ttgaaaatag gggccttggg tcggaactga gtaagcctgg
     6121 cgtgctggca tcccaggtag acagcccgtt ttcgggctgc ttcgaggatc ttgccatcag
     6181 tgccagcacc tccttgggca tgggaccatg ccatggccca gaggagaatg agtataagag
     6241 tgaaggcact ttcgggatcc acgtcgctga aaacccaagc atccagcttc tggaaggcaa
     6301 ccctgggcca cctgcggacc ctgatggagg cccaagacca caagccgacc ggaagttcca
     6361 ggagagggaa gtgccatgcc acaggccctc acctggggct ctgtggctcc aggtggctgt
     6421 gacaggggtg ctggtagtca cactcctggt ggtgctgtac cggcggcgtc tgcacgggtc
     6481 cggctcacgt gaccacatgg tccttcatga gtatgtaaat gctgctggga ttacataact
     6541 cgagtctaga gggcccgttt aaacccgctg atcagcctcg acctgcaggc atgcaagctt
     6601 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6661 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6721 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6781 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6841 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6901 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6961 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7021 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7081 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7141 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7201 tccccgcatc gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc
     7261 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7321 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7381 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7441 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7501 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     7561 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     7621 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     7681 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     7741 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     7801 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     7861 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     7921 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     7981 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     8041 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     8101 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     8161 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     8221 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     8281 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     8341 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8401 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8461 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8521 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     8581 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     8641 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     8701 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8761 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8821 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8881 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8941 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9001 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9061 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9121 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9181 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9241 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9301 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9361 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9421 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9481 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9541 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9601 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9661 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9721 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9781 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9841 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9901 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9961 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10021 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10081 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10141 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    10201 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    10261 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    10321 ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
    10381 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
    10441 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    10501 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
    10561 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
    10621 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    10681 aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc
    10741 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
    10801 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
    10861 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
    10921 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
    10981 cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat
    11041 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg
    11101 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt
    11161 ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca
    11221 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg
    11281 attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct
    11341 tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt
    11401 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt
    11461 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc
    11521 acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat
    11581 taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc
    11641 acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg
    11701 ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact
    11761 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
    11821 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
    11881 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
    11941 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
    12001 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
    12061 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
    12121 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
    12181 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
    12241 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
    12301 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
    12361 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    12421 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    12481 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    12541 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    12601 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    12661 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    12721 tgtatttgtt aaatatgtac tacaaactta gtagttgg
//
