LOCUS       sequence-450159-       13047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Condensate module, light-inducible oligomerization IDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13047)
  AUTHORS   Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, 
            Brangwynne CP
  TITLE     Condensate interfacial forces reposition DNA loci and probe 
            chromatin viscoelasticity.
  JOURNAL   Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 
            10.1016/j.cell.2024.07.034.
  PUBMED    39168125
REFERENCE   2  (bases 1 to 13047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034."
COMMENT     Sequence Label: BRD4dN-mCherry-SspB
FEATURES             Location/Qualifiers
     source          1..13047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2852..3032
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     3537..3553
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3538..3554
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3557..4145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3663..3674
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4072..4092
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4216..4545)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     CDS             complement(7153..7170)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(8051..9262)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(8195..8214)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    9327..9444
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9742..9786)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9971..10204
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10697..10822
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10869..11049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(11067..11265)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(11092..11112)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        11267..11646
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     11818..11837
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11912..12016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12017..12085,12086..12877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12235..12254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag
     2881 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     2941 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     3001 caggctcaga tctggtctaa ccagagagac ccagtacagt ccggatgcag ctctcgggcc
     3061 atgtgatgaa atgctaggcg gctgtcaaac ctccactcta atacttctct ctccgggtca
     3121 tccatcccat gcaggctcac agggtgtaac aagcgggtgt tctctccttc attggcttct
     3181 tctaccttct cttgctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat
     3241 ctgatccctg gccctggtgt gtagttctgc caatcaggga agtagccttg tgtgtggtag
     3301 atccacagat caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     3361 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
     3421 acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag ccaggcacaa
     3481 tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga
     3541 ggtcgacggt atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     3601 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     3661 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     3721 caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg
     3781 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     3841 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     3901 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     3961 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     4021 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     4081 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     4141 tgattatcga taagcttgat atcgaattct agagtcgcgg ccgctttact tgtacgatcc
     4201 cggcgcgcct taaccaatat tcagctcgtc atagatttct tctggttcga acatcacacc
     4261 atcgccgttc tcgcgagcgt aaatggccag agcggcaccc atcgggatat acagttcacg
     4321 agacacgccc ttaaagcggg cgttgaactg gataaaatca tttgtcagtt gcaggttgcc
     4381 ggtcgcactt gcagacagat tcagcacgat ctgaccgtct ttcacatact ccacgggcac
     4441 gttcacgccc aggtatgtgg catccaccac cagatatggg gtaaagctgt tatcaaccag
     4501 ccaatcgtaa tattcacgca gcagcttagg gcgtttcggg gagctacctc cagaggtacc
     4561 accggatccc ttgtacaatt catccatgcc ccctgtgcta tgtctgccct cggcccgctc
     4621 gtactgttcc acgatggtat agtcttcgtt atgggatgta atatccagtt tgatattcac
     4681 gttgtatgcc ccaggaagct gaacgggttt cttggcctta taggtggttt tgacctcagc
     4741 atcataatgt cccccatcct tcagcttgag tcgctgcttg atttctccct tcagtgctcc
     4801 gtcctcggga tacatgcgtt ctgagctggc ttcccagccc atagttttct tctgcatcac
     4861 ggggccgtct gatgggaagt tagtgccccg aagtttaacc ttatagataa attctccgtc
     4921 ctgaagagag ctgtcctggg taactgtgac tacaccgcca tcctcaaaat tcattacccg
     4981 ttcccacttg aacccttcag ggaagctcag tttcaagtag tccggaatat cggcagggtg
     5041 cttaacgtaa gccttagagc cgtacataaa ctggggtgac aggatgtccc aggcgaaagg
     5101 cagggggcca cccttggtca ccttcagttt ggccgtctgt gtcccttcat agggtcgccc
     5161 ctcgccttcg ccctcgattt cgaactcgtg accgttcaca gatccctcca tatgcacctt
     5221 gaacctcata aattccttga ttatagccat gttatcctcc tcgcctttag acaccattcc
     5281 tccacctccg ctagagaaaa gattttcttc aaatattgac aatagatcac tctggaaatt
     5341 catgtcaatg gtagctgcca tggcttcccg gcgtcttcgc tcctgctccc gcttccgggc
     5401 caactccctc tgctggtcca gcatggactg gggctgggag ctctgggcct gtggggtggc
     5461 ggcggcagcc accgcagctg cttgctgttg ctgctgctgc tgttgctcct ggcgctgctg
     5521 ctgctgctgc tcctggcgcc gacgtgcctc ctcatgggcc cgccgggcct gctccagcgc
     5581 atcctcgtcc tctcggctcc tcatgcgctc ctgccgcagc cgctccttct ccttctcagc
     5641 gtgctcggcc tgagccttca gggccttctc acgctcctct ttctcccgag cggcgcggcg
     5701 gaactgctcg aagctgtcgc tggatgactt ggctgtggag gagggggtgg tcggatgctt
     5761 ctgcactagg ctggcccagg agcccatgtt cttgattttc aggtcctttt tgggcgcaac
     5821 tggagtcttc ggctcctgtt tctgtttgtc cttgtcaggg gcccctggtg gcggtgcgtt
     5881 ctgctctggg ggccggatca caggcctccc gacatccaca ggcttcattt ccggccgctg
     5941 gggcaggtgg acgggggcct tgatgctctc cgggtgcttg gggggctccg gccgcagcga
     6001 ggggctgaag ggctcgctgc ggatgatggg tgagtggatc ttctcctcct tcaccaccac
     6061 gaggggctgg ggctggacca cggaggcagc acgcagctcc tgtttcttag gctggacgtt
     6121 ttgctggggt ggagactggt gggtcaggct ctggaactgt gacatctggg gggaatgtat
     6181 cataagcggg gagggggctt cgcggaggtg accggttgag taggggtccg acttgtggtg
     6241 ccggggtgaa tggtggtgct ggatgacttg ctgaggcttg gcaggctgcg gcgggggtgg
     6301 ctgctggcct gggggcggat gggggggctg ctggccctgg ggtggcgggg gctgttggat
     6361 gtgggtggaa aactgcatgg gctgcaagtg cacgggccgt ggagggggct gatgctgctg
     6421 ctggggtgga ggctggggct ggggtggtgg gggtggtggc ggctgctgct gcagctgctg
     6481 ctgcacagag gggtggggtg ggggcggcag ggggggtggg ggctgggact gcaccttcac
     6541 ggaagggagt agcggcgtag ggggctgcac cttctgcagc tgctgcaggt acagctgcat
     6601 ctgcatggag gtgaggggtg gggcaggtgg ctcttcatcc tccagcagca cttggggggg
     6661 ttgggccatg gggggctgtg ggagcagggg tgtttgggtc aaggctggtg acacggctgg
     6721 gggccgggcg ggcttgggag gcagggcagc ggctcggttg ctgggccgtg atggctgctg
     6781 gggtagtgcg ttgtgcaaag ctggaggaga gaccactgcg tgctggttga gatggggtgg
     6841 agtgctgtgc tcaggcggct ggggcaggtg agggggcagc tcaggctgcg gcaggtgcag
     6901 gatgggctgg gtgaagtggc cgatggggtc aaagacgctg cctgggagct ggggctccag
     6961 gacgggcacc tgggtggcaa tgaagggtgg gggcgaggac ttcatcgccg gggctgcctg
     7021 ctgcggcatg gagggtgggg gaggcggggg tggcggctgc tgttgctgct gcggaggtgg
     7081 aggcggtggg ggctgctggg gaggcggggg cggctgctgg ggcacaggag ccggggcctg
     7141 ctgcatctgc tgatggtggt gatgatggtg cttcttctgc tccctcccgg ggtgcccctt
     7201 cttttttgac ttcggagcca tctctgtttc ggagtcttcg ctgtcagagg agctggactc
     7261 actggagctc tccgactctg aggacgagaa gcccttcatc ttggaggagc cggcaatcac
     7321 atcaactttc tcagcttgag gtttcctttt cttccgcaaa caggaggtga catagcgctc
     7381 cagctcacgc agtgtggacg gcttcagggt ctcaaagtcg atttcaatct cgtcggggtt
     7441 ggaattcttc agggagggct cccgtgactg gatgatgtgc accacgcggc ccagcttctc
     7501 gccggggagc ttgttgatgt ccaagctgag ctgccgcttc tcctcatagg acataggctt
     7561 gcacttgtcc tcttcctccg actcatacgt gggagggggc ttgctcttca tgggcgctgg
     7621 ctccttcttg ctcacattgc tgttgctgct attatttttc ttcgtctttt taggaggagg
     7681 ttccttggct ttgctttttt tattctcttc cacttcctct ttccttttgt gcttttcttt
     7741 tttcttttcc ttcttgtctt tctccttttt ctttggtttg ttctgctggg gctgagagag
     7801 ggctgcaagc tgctcgtgca cggctttgag ctgctcctgg agctcagcca gccgctgggc
     7861 tcgctcctcc tcagagtcat cagtcgaact gtcactgtcc gaggagctat cgctgctgct
     7921 gtcgctggat gagggcgggg ccacaacctt ggtgggaggg ggcactgccg gggaggacac
     7981 ggccaccact ggctcctcca tggtgctagc taccggtgat cctctagagt cgacctgcag
     8041 cccaagcttc gtctaacaaa aaagccaaaa acggccagaa tttagcggac aatttactag
     8101 tctaacactg aaaattacat attgacccaa atgattacat ttcaaaaggt gcctaaaaaa
     8161 cttcacaaaa cacactcgcc aaccccgagc gcatagttca aaaccggagc ttcagctact
     8221 taagaagata ggtacataaa accgaccaaa gaaactgacg cctcacttat ccctcccctc
     8281 accagaggtc cggcgcctgt cgattcagga gagcctaccc taggcccgaa ccctgcgtcc
     8341 tgcgacggag aaaagcctac cgcacaccta ccggcaggtg gccccaccct gcattataag
     8401 ccaacagaac gggtgacgtc acgacacgac gagggcgcgc gctcccaaag gtacgggtgc
     8461 actgcccaac ggcaccgcca taactgccgc ccccgcaaca gacgacaaac cgagttctcc
     8521 agtcagtgac aaacttcacg tcagggtccc cagatggtgc cccagcccat ctcacccgaa
     8581 taagagcttt cccgcattag cgaaggcctc aagaccttgg gttcttgccg cccaccatgc
     8641 cccccacctt gtttcaacga cctcacagcc cgcctcacaa gcgtcttcca ttcaagactc
     8701 gggaacagcc gccattttgc tgcgctcccc ccaaccccca gttcagggca accttgctcg
     8761 cggacccaga ctacagccct tggcggtctc tccacacgct tccgtcccac cgagcggccc
     8821 ggcggccacg aaagccccgg ccagcccagc agcccgctac tcaccaagtg acgatcacag
     8881 cgatccacaa acaagaaccg cgacccaaat cccggctgcg acggaactag ctgtgccaca
     8941 cccggcgcgt ccttatataa tcatcggcgt tcaccgcccc acggagatcc ctccgcagaa
     9001 tcgccgagaa gggactactt ttcctcgcct gttccgctct ctggaaagaa aaccagtgcc
     9061 ctagagtcac ccaagtcccg tcctaaaatg tccttctgct gatactgggg ttctaaggcc
     9121 gagtcttatg agcagcgggc cgctgtcctg agcgtccggg cggaaggatc aggacgctcg
     9181 ctgcgccctt cgtctgacgt ggcagcgctc gccgtgagga ggggggcgcc cgcgggaggc
     9241 gccaaaaccc ggcgcggagg ccagatctgg agccgacacg ggttaattaa ccaaactgga
     9301 tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat
     9361 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
     9421 ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct gcagaattgg
     9481 cgcacgcagt gccgatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc
     9541 ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa
     9601 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     9661 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     9721 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     9781 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     9841 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     9901 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     9961 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
    10021 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
    10081 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
    10141 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
    10201 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
    10261 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
    10321 cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac
    10381 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
    10441 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
    10501 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
    10561 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
    10621 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
    10681 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
    10741 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
    10801 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
    10861 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
    10921 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
    10981 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
    11041 gagagaccca gtacaggcaa aacgcgctgc ttatatagac ctcccaccgt acacgcctac
    11101 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
    11161 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
    11221 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
    11281 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
    11341 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
    11401 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
    11461 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
    11521 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
    11581 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
    11641 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
    11701 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
    11761 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
    11821 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
    11881 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    11941 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    12001 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    12061 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    12121 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    12181 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    12241 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    12301 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    12361 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    12421 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    12481 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    12541 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    12601 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    12661 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    12721 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    12781 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    12841 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    12901 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    12961 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    13021 ccccgtagaa aagatcaaag gatcttc
//
