LOCUS       sequence-450161-        8879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adhesion module, guide RNA recognizes telomeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, 
            Brangwynne CP
  TITLE     Condensate interfacial forces reposition DNA loci and probe 
            chromatin viscoelasticity.
  JOURNAL   Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 
            10.1016/j.cell.2024.07.034.
  PUBMED    39168125
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034."
COMMENT     Sequence Label: sgTelo
FEATURES             Location/Qualifiers
     source          1..8879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2057..2755)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(2768..2821)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2831..3427)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2911..2931)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3408..3427
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     intron          3448..4386
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3471..3491)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(4387..4598)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(4845..4865)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    4869..4902
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5155..5272
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5570..5614)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5799..6032
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6529..6654
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6701..6881
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6899..7097)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6924..6944)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7099..7478
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7650..7669
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7744..7848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7849..7917,7918..8709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8067..8086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcttaa ttaagcttgt gccccagttt gctagggagg tcgcagtatc
     2101 tggccactgc cacctcgtgc tgctcgacgt aggtctcgtt gttggcctcc ttgattcttt
     2161 ccagtctgta gtccacatag tagacgccag gcatcttgag gttcttagcg ggtttcttgg
     2221 atctatatgt ggtcttgatg tttgcgatca gatggctccc gcccacgagc ttcagggcca
     2281 tgtcgtttct gccttccagg ccgccgtcag cggggtacag cgtctcggtg aaggcctccc
     2341 agccgagtgt tttcttctgc atcacagggc cgttggatgt gaagttcacc cctctgatct
     2401 tgacgttgta gatgaggcag ccgtcctgga ggctggtgtc ctgggtagcg gtcagcacgc
     2461 ccccgtcttc gtatgtggtg actctctccc atgtgaagcc ctcagggaag gactgcttga
     2521 agaagtcggg gatgccctgg gtgtggttga tgaaggtctt gctgccgtag aggaagctag
     2581 tagccaggat gtcgaaggcg aaggggagag ggccgccctc gaccaccttg attctcatgg
     2641 tctgggtgcc ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag tgatggttgt
     2701 ccacggtgcc ctccatgtac agcttcatgt gcatgttctc cttaatcagc tcgctggtgg
     2761 cgaccggtgg gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct
     2821 ctcccgatcc ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     2881 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     2941 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     3001 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
     3061 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     3121 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     3181 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     3241 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     3301 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     3361 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     3421 cggtcatggt gggctagctc acgacacctg aaatggaaga aaaaaacttt gaaccactgt
     3481 ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg gcaaattcca aggagaatta
     3541 catcaagtgc caagctggcc taacttcagt ctccacccac tcagtgtggg gaaactccat
     3601 cgcataaaac ccctcccccc aacctaaaga cgacgtactc caaaagcgcg agaactaatc
     3661 gaggtgcctg gacggcgccc ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg
     3721 aaaggccctt ttcctttgtg tgggtgactc acccgcccgc tctcccgagc gccgcgtcct
     3781 ccattttgag ctccctgcag cagggccggg aagcggccat ctttccgctc acgcaactgg
     3841 tgccgaccgg gccagccttg ccgcccaggg cggggcgata cacggcggcg cgaggccagg
     3901 caccagagca ggccggccag cttgagacta cccccgtccg attctcggtg gccgcgctcg
     3961 caggccccgc ctcgccgaac atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc
     4021 cccaaaaacc gaaataccag tgtgcagatc ttggcccgca tttacaagac tatcttgcca
     4081 gaaaaaaagc gtcgcagcag gtcatcaaaa attttaaatg gctagagact tatcgaaagc
     4141 agcgagacag gcgcgaaggt gccaccagat tcgcacgcgg cggccccagc gcccaggcca
     4201 ggcctcaact caagcacgag gcgaaggggc tccttaagcg caaggcctcg aactctccca
     4261 cccacttcca acccgaagct cgggatcaag aatcacgtac tgcagccagg tggaagtaat
     4321 tcaaggcacg caagggccat aacccgtaaa gaggccaggc ccgcgggaac cacacacggc
     4381 acttacctgt gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg cgactactgc
     4441 acttatatac ggttctcccc caccctcggg aaaaaggcgg agccagtaca cgacatcact
     4501 ttcccagttt accccgcgcc accttctcta ggcaccggtt caattgccga cccctccccc
     4561 caacttctcg gggactgtgg gcgatgtgcg ctctgcccac tgacgggcac atgcatggcg
     4621 gtaatacggt tatccacgcg gccgcctaat ggatcctagt actcgagaaa aaaagcaccg
     4681 actcggtgcc actttttcaa gttgataacg gactagcctt atttaaactt gctatgctgt
     4741 ttccagcata gctcttaaac taaccctaac cctaacccta accaacaagg tggttctcca
     4801 agggatactt atagtctcaa aacacacaat tactttacag ttagggtgag tttccttttg
     4861 tgctgtttat aacttcgtat aatgtatgct atacgaagtt atagaaatcc aagcctatca
     4921 tgtaaaatgt agctagtatt aaaaagaaca gattatctgt cttttatcgc acattaagcc
     4981 tctatagtta ctaggaaata ttatatgcaa attaaccggg gcaggggagt agccgagctt
     5041 ctcccacaag tccgtgcgag ggggccggcg cgggcctaga gatggcgcgt cggatctcta
     5101 gagcgggccc ggtactaacc aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt
     5161 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     5221 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     5281 gaatactgcc atttgtctgc agaattggcg cacgcagtgc cgatccgttc actaatcgaa
     5341 tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc
     5401 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
     5461 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
     5521 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
     5581 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
     5641 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
     5701 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
     5761 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
     5821 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
     5881 acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag
     5941 acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg
     6001 ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca
     6061 ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat
     6121 atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca
     6181 gcggccggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc
     6241 taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac
     6301 tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc
     6361 cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc
     6421 tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct
     6481 taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca
     6541 cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg
     6601 cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc
     6661 tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact
     6721 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     6781 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     6841 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct
     6901 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     6961 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     7021 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     7081 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     7141 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     7201 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     7261 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     7321 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     7381 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     7441 aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac
     7501 atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa
     7561 gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca
     7621 agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     7681 ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat
     7741 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7861 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7921 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7981 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8041 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8101 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8161 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8221 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8281 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8341 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8401 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8461 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8521 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8581 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8641 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8701 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8761 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
