LOCUS       sequence-450167-       12649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adhesion module, creates 'seed' at Cajal body.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12649)
  AUTHORS   Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, 
            Brangwynne CP
  TITLE     Condensate interfacial forces reposition DNA loci and probe 
            chromatin viscoelasticity.
  JOURNAL   Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 
            10.1016/j.cell.2024.07.034.
  PUBMED    39168125
REFERENCE   2  (bases 1 to 12649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034."
COMMENT     Sequence Label: FUS-miRFP670-Coilin
FEATURES             Location/Qualifiers
     source          1..12649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    210..443
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    936..1061
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1108..1288
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1306..1504)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1331..1351)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1506..1885
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2057..2076
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2151..2255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2256..2324,2325..3116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2474..2493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3287..3875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3776..3795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4029..4046
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4163..4184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4199..4204,4205..4222,4223..4229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4237..4253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4242..4264
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4261..4277
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4261..4277
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4326..4447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4337..4356)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4391..4410
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4577..4951)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(4970..5017)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5065..5394)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5079..5214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5133..5152)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5379..5399
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(5408..5836)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5518..5539)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5730..5749
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5882..6106
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6095..6112
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             6138..6318
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     6823..6839
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6824..6840
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6843..7431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6949..6960
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7358..7378
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9261..10205)
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             complement(10495..10506)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      10883..10892
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10939..12150)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(11083..11102)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    12215..12332
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(join(12630..12649,1..25))
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
       61 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
      121 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
      181 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
      241 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
      301 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
      361 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
      421 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
      481 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
      541 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct
      601 tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg
      661 gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat
      721 gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta
      781 cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt
      841 gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt
      901 tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc
      961 ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt
     1021 gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc
     1081 gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc
     1141 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     1201 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     1261 ctcagatctg gtctaaccag agagacccag tacaggcaaa acgcgctgct tatatagacc
     1321 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     1381 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     1441 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     1501 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     1561 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     1621 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     1681 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     1741 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     1801 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     1861 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
     1921 gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt
     1981 gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga
     2041 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg
     2101 ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3421 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3901 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4021 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     4081 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4141 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4201 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4261 caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac ggtatacaga
     4321 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4381 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4441 acaagttggg gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     4501 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     4561 aatctcgtag cacgtgtcag tcctgctcct cggccacgaa gtgcacgcag ttgccggccg
     4621 ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc atggccggcc
     4681 cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac agctcgtcca
     4741 ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc tggaccgcgc
     4801 tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc acgaagtccc
     4861 gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga
     4921 gcaccggaac ggcactggtc aacttggcca tggtttagtt cctcaccttg tcgtattata
     4981 ctatgccgat atactatgcc gatgattaat tgtcaacacg tgctgatcag atccgaaaat
     5041 ggatatacaa gctcccggga gctttttgca aaagcctagg cctccaaaaa agcctcctca
     5101 ctacttctgg aatagctcag aggcagaggc ggcctcggcc tctgcataaa taaaaaaaat
     5161 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     5221 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     5281 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     5341 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     5401 cagaattaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     5461 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     5521 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     5581 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     5641 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     5701 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     5761 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     5821 ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa
     5881 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     5941 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     6001 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     6061 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     6121 cgggtttaaa cgggccctgc tagagatttt ccacactgac taaaagggtc tgagggatct
     6181 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     6241 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     6301 gtctaaccag agagacccag tacagtccgg atgcagctct cgggccatgt gatgaaatgc
     6361 taggcggctg tcaaacctcc actctaatac ttctctctcc gggtcatcca tcccatgcag
     6421 gctcacaggg tgtaacaagc gggtgttctc tccttcattg gcttcttcta ccttctcttg
     6481 ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga tccctggccc
     6541 tggtgtgtag ttctgccaat cagggaagta gccttgtgtg tggtagatcc acagatcaag
     6601 gatatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa
     6661 gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtacct gaggtgtgac
     6721 tggaaaaccc acctcctcct cctcttgtgc ttctagccag gcacaatcag cattggtagc
     6781 tgctgtattg ctacttgtga ttgctccatg tttttctagg tctcgaggtc gacggtatcg
     6841 atgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
     6901 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
     6961 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
     7021 aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
     7081 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
     7141 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
     7201 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     7261 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     7321 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     7381 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tatcgataag
     7441 cttgatatcg aattctagag tcgcggccgc tttacttgta cgatccggcg cgatcattag
     7501 gcaggttctg tacttgatgt gttacttgga gattcaataa tcagtcttgg gtcaatcaac
     7561 tctttccaaa atacagtgat cttgctctcc tgtgtcacag cgtactccac tacctcagct
     7621 ccattttcat tgtgataaac taaatcaaat ttcccaggtt ctctcaaggc aggtaaggat
     7681 gaaagaattt ctatatctac ttgctgggtc tctggattgt ggcttaatat tcttccttcc
     7741 ttgtagtcag agacatcagg agagtaactg gatgttagct ccaaaagctt aaatgcaatc
     7801 ttttctccaa cttgaggggc agctgctaac agtggtaaca gactatagtc cttcttgggt
     7861 gtctctactg gattctggat aatagtagat gaatttttta ccacgtcatt taattgctgt
     7921 tgcctctggt tgtcagtgct tctatttaca acacaggaaa caggatgccc tcgtcctcga
     7981 cctctccccc gcatgccccg tcccttagct cccttccaag aaaaaaggtt ctcttctcta
     8041 ccccatcctc ttcctaaact agcagggaga gacacactgg gagaagcacc aggagcctgt
     8101 ccaccaccat ttgagccaga acgcctccat ccagcagcac ctgcagtctg tgatgacagc
     8161 cctgggcctg gacgacctct tcctgcaaaa aggcctactg tctttaagaa acccgcagca
     8221 cactccgggg tgctcgatga catcaagcat tggtcgtctg actctgcact agagtctgaa
     8281 ctggaagatg ttgttccaga ggtcttgccc ttgctggggg taaggctaaa gccaagtttt
     8341 atagccagtt tgtctgcagt tgtatttttg gtagagggtt cttcctttga taactcagtt
     8401 ggtaatttct ctgaggaatt tctggcctcc aaagtgactt tgctgggacc atcactgata
     8461 gattcatcac aagactcaga ctccgaggag gaactgggac tctcttttga gcaaacgctt
     8521 acactacctt tccttttggc tttaacaagg ctgtttctag cagaaccttt tggagaacta
     8581 catctctgat tggcccagtc tttcactgcc tgtactttcg gagacttggg attcttagcc
     8641 ttttttttat attcacattt ctcctttttc tttggtgatt ttcttttggc ctcttcgtta
     8701 tcatcaccca ctgtgccaca ggttgcttta ttttttctct tgtttttttt gctgacagtc
     8761 tgatctgtga cagcttttgg ttccagatcc aagaccttct cattattgtt attgttctct
     8821 cgactcttcc aatgcttctt tgaatatttg caatctggtt cagtttcttc accctcctct
     8881 aactgaaatg cccgcttctt tgcttttcta agagataaat taatgtcacc attactgatg
     8941 actacagaat tctcagcaac tcctctctct tctaatttaa ctctgaggca gtcgttgtct
     9001 ctcacaaggc gcgcgctctc ggcggggggc aagagccccc cctccaggta gaggcctagg
     9061 aaggccccag aactgaagcc gaagcgctgg cggatgagac taatgagatc tgtgacgact
     9121 cggcatctgt tcaagtcgac cagaagccag aaggccgtac agtgcggggt agctggcggc
     9181 gggtaatcaa attgaagccg tagcctaacc gtctcggaag ctgccgcgcc tccagatccg
     9241 ccggatccgc cgcctccgga gctctcaagc gcggtgatcc gcgtcgcgat cctttcggcg
     9301 agccgtttgc agatcgcccg cagctcgaaa cggatgaagc gcggcagata atggtgacag
     9361 acaaccaggc cccacagctt gccgccgacc accagcgaca ccgccagggt ggcgcgcacg
     9421 cccatgtcct tcaggaactg cagatggcac ggcgacatcg agcgcaggaa gcagcccgac
     9481 atgtcgagat cgcgcccggt cagcggcgac agccgcggct ccagcggcac cggctgatag
     9541 gtgacgtcga ccagcacgcg gacgcgctgc cgcacgtaca gctgccgcgc catctgcggg
     9601 acagtcgacg acggatagcg gttgccgaaa taggattcga gcccaggcac atggcactcg
     9661 gagaatacca ggccgtggcc ttgctcatcg aaacgataca ccatcacccg gtcgtagccg
     9721 gtgcactgct gaaacagcag cacggtgtca tcgcacagcg cgcgcagtga acccgccgtg
     9781 cggatccgct ccagcgccgg cgccagcgtg cctgacagat cgatcgacgg gccggcacgt
     9841 tcgagttcga tgatcagccc gccttccgga ggccgatgca tcagaccgca gtactccgta
     9901 gagggattgc cgatccggca gcgcaccgcg accggcatgc cttcggcggt gggatcgaga
     9961 tgcggcagga tcttgatcaa cagatcgccg tcgatctcgg cgagcggaac gccgagtacg
    10021 cttccgagat tcagaaattc cgcggcgttg gcgctggcct ggatgacgcg atgatcatgt
    10081 tcgctgacga ccagaagcgc gccatgcggc tggatcgagc cggcgaggtg gatctcttca
    10141 tgttcgcaat tcgaatgaga ggcggtcccg aatgcggggc tgccagaggc atgacctgct
    10201 accatacctg atccggatcc tgaagcagaa ccggagccag atcctccacg gtcctgctgt
    10261 ccatagccac cgctgccacc tccaccactc tggtcttgat tgccataacc gccaccactg
    10321 ccaccaccac tactcatgga ggattgatct tggccatagt tacctccacc tccacctcca
    10381 cctccaccac cactgctgct gttgtactgg ttctgctgtc catagccctg agggggatta
    10441 tagctttgct gctgtccata gctttgctgc tgtccaccat agctaggctg ctggctgtag
    10501 ctcccactct ggggctgccc atagctgctg ctctgagaac tgctaccgta acttcccgag
    10561 gtgctgctgg gagctggctg ctggccatag ccaggatagg aggactgctg cccgtaagac
    10621 gattgggagc tctggctact gccatagccg ccagtcgagc catatccctg gggagttgac
    10681 tgagttccat agcctgtgtt ctggctctgg ccataagaag aatagctgct ctggccatag
    10741 cctgaagtgt ccgtggactg gctataacca ctgtaactct gctgtccgta gggctgactg
    10801 ctctgctggg aatagccctg cccgggctgg gtggggtagg ccccatagct ttgggttgct
    10861 tgttgggtat aatcgtttga ggccatggtg gcgctagcta ccggtgatcc tctagagtcg
    10921 acctgcagcc caagcttcgt ctaacaaaaa agccaaaaac ggccagaatt tagcggacaa
    10981 tttactagtc taacactgaa aattacatat tgacccaaat gattacattt caaaaggtgc
    11041 ctaaaaaact tcacaaaaca cactcgccaa ccccgagcgc atagttcaaa accggagctt
    11101 cagctactta agaagatagg tacataaaac cgaccaaaga aactgacgcc tcacttatcc
    11161 ctcccctcac cagaggtccg gcgcctgtcg attcaggaga gcctacccta ggcccgaacc
    11221 ctgcgtcctg cgacggagaa aagcctaccg cacacctacc ggcaggtggc cccaccctgc
    11281 attataagcc aacagaacgg gtgacgtcac gacacgacga gggcgcgcgc tcccaaaggt
    11341 acgggtgcac tgcccaacgg caccgccata actgccgccc ccgcaacaga cgacaaaccg
    11401 agttctccag tcagtgacaa acttcacgtc agggtcccca gatggtgccc cagcccatct
    11461 cacccgaata agagctttcc cgcattagcg aaggcctcaa gaccttgggt tcttgccgcc
    11521 caccatgccc cccaccttgt ttcaacgacc tcacagcccg cctcacaagc gtcttccatt
    11581 caagactcgg gaacagccgc cattttgctg cgctcccccc aacccccagt tcagggcaac
    11641 cttgctcgcg gacccagact acagcccttg gcggtctctc cacacgcttc cgtcccaccg
    11701 agcggcccgg cggccacgaa agccccggcc agcccagcag cccgctactc accaagtgac
    11761 gatcacagcg atccacaaac aagaaccgcg acccaaatcc cggctgcgac ggaactagct
    11821 gtgccacacc cggcgcgtcc ttatataatc atcggcgttc accgccccac ggagatccct
    11881 ccgcagaatc gccgagaagg gactactttt cctcgcctgt tccgctctct ggaaagaaaa
    11941 ccagtgccct agagtcaccc aagtcccgtc ctaaaatgtc cttctgctga tactggggtt
    12001 ctaaggccga gtcttatgag cagcgggccg ctgtcctgag cgtccgggcg gaaggatcag
    12061 gacgctcgct gcgcccttcg tctgacgtgg cagcgctcgc cgtgaggagg ggggcgcccg
    12121 cgggaggcgc caaaacccgg cgcggaggcc agatctggag ccgacacggg ttaattaacc
    12181 aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt
    12241 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
    12301 tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtctgc
    12361 agaattggcg cacgcagtgc cgatccgttc actaatcgaa tggatctgtc tctgtctctc
    12421 tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg
    12481 gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga aagtacagca
    12541 aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt tatataccac
    12601 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatcta
//
