LOCUS       sequence-45023-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Sheng Y, Laister RC, Lemak A, Wu B, Tai E, Duan S, Lukin J, 
            Sunnerhagen M, Srisailam S, Karra M, Benchimol S, Arrowsmith CH
  TITLE     Molecular basis of Pirh2-mediated p53 ubiquitylation.
  JOURNAL   Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42.
  PUBMED    19043414
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2008 Dec . 15(12):1334-42."
COMMENT     Sequence Label: p53-(1-393)-R273H-N268D
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             24..46
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             65..82
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..109
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tgaggaaatt ccctctgaat aattttgttt aactttaaga aggagatata ccatgggcag
       61 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatggagga
      121 gccgcagtca gatcctagcg tcgagccccc tctgagtcag gaaacatttt cagacctatg
      181 gaaactactt cctgaaaaca acgttctgtc ccccttgccg tcccaagcaa tggatgattt
      241 gatgctgtcc ccggacgata ttgaacaatg gttcaccgaa gacccaggtc cagatgaagc
      301 tcccagaatg ccagaggctg ctccccccgt ggcccctgca ccagcagctc ctacaccggc
      361 ggcccctgca ccagccccct cctggcccct gtcatcttct gtcccttccc agaaaaccta
      421 ccagggcagc tacggtttcc gtctgggctt cttgcattct gggacagcca agtctgtgac
      481 ttgcacgtac tcccctgccc tcaacaagat gttttgccaa ctggccaaga cctgccctgt
      541 gcagctgtgg gttgattcca cacccccgcc cggcacccgc gtccgcgcca tggccatcta
      601 caagcagtca cagcacatga cggaggttgt gaggcgctgc ccccaccatg agcgctgctc
      661 agatagcgat ggtctggccc ctcctcagca tcttatccga gtggaaggaa atttgcgtgt
      721 ggagtatttg gatgacagaa acacttttcg acatagtgtg gtggtgccct atgagccgcc
      781 tgaggttggc tctgactgta ccaccatcca ctacaactac atgtgtaaca gttcctgcat
      841 gggcggcatg aaccggaggc cccatcctca ccatcatcac actggaagac tccagtggta
      901 a
//
