LOCUS       sequence-45025-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Sheng Y, Laister RC, Lemak A, Wu B, Tai E, Duan S, Lukin J, 
            Sunnerhagen M, Srisailam S, Karra M, Benchimol S, Arrowsmith CH
  TITLE     Molecular basis of Pirh2-mediated p53 ubiquitylation.
  JOURNAL   Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42.
  PUBMED    19043414
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2008 Dec . 15(12):1334-42."
COMMENT     Sequence Label: p53-(1-393)-E285V-N268D
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggtgccgcgc ggcagccata tggaggagcc gcagtcagat cctagcgtcg
      121 agccccctct gagtcaggaa acattttcag acctatggaa actacttcct gaaaacaacg
      181 ttctgtcccc cttgccgtcc caagcaatgg atgatttgat gctgtccccg gacgatattg
      241 aacaatggtt caccgaagac ccaggtccag atgaagctcc cagaatgcca gaggctgctc
      301 cccccgtggc ccctgcacca gcagctccta caccggcggc ccctgcacca gccccctcct
      361 ggcccctgtc atcttctgtc ccttcccaga aaacctacca gggcagctac ggtttccgtc
      421 tgggcttctt gcattctggg acagccaagt ctgtgacttg cacgtactcc cctgccctca
      481 acaagatgtt ttgccaactg gccaagacct gccctgtgca gctgtgggtt gattccacac
      541 ccccgcccgg cacccgcgtc cgcgccatgg ccatctacaa gcagtcacag cacatgacgg
      601 aggttgtgag gcgctgcccc caccatgagc gctgctcaga tagcgatggt ctggcccctc
      661 ctcagcatct tatccgagtg gaaggaaatt tgcgtgtgga gtatttggat gacagaaaca
      721 cttttcgaca tagtgtggtg gtgccctatg agccgcctga ggttggatct gactgtacca
      781 ccatccacta caactacatg tgtaacagtt cctgcatggg cggcatgaac
//
